Identificación de nuevos genes implicados en la iniciación y desarrollo de las raíces laterales en "Arabidopsis thaliana"

Las plantas son organismos sésiles que deben crecer en el medio donde germinan y adaptarse a las condiciones ambientales que les rodean. El sistema radicular es fundamental para su adaptación y eficiencia reproductiva, ya que es el órgano encargado de adquirir nutrientes y agua del medio. El incremento del sistema radicular está determinado por el número y posición de las raíces laterales. Entender cómo las plantas reorganizan su sistema radicular, permitirá obtener plantas que requieran una menor aportación de recursos. En este trabajo se han identificado nuevos genes implicados en la formación y desarrollo de las raíces laterales en “Arabidopsis thaliana” utilizando una estrategia genómica y otra genética. Con la primera, mediante una estrategia de “separación celular” y genómica, se han identificado más de 600 genes relacionados con este proceso y se ha analizado la función de un grupo de ellos que se seleccionaron en base a su expresión tisular. Mediante una estrategia genética, se ha identificado un mutante recesivo, “srol1”, que presenta un sistema radicular reducido dependiente de la calidad de la luz, del cual se ha llevado a cabo su caracterización fenotípica y molecular.Plants are sessile organisms that have to adapt their growth to the environment where they are obligated to live. To have an optimal adaptation, the root system plays a key role, because acquires the nutrients and water needed for the efficient growth and development of the plants. The growth of the root system is based on the length of the main root and on the number and disposition of the lateral roots. Understanding how plants can adapt (re-organize) their root system will allow us to get plants that require less input of resources. In this project we have identified new genes that are implicated in the lateral root formation and development in “Arabidopsis thaliana”. Using an approach based on the combination of specific cell sorting and transcriptomic we have identified over 600 genes that are involved in the development of the root system. We have analyzed the function of a set of these genes selected on bases of their tissue expression. Using a genetic approach, we have identified a recessive mutant, “srol1”, that shows a reduced root development that is dependent on the quality of the light perceived by the root system. We have carried out a phenotypic and molecular characterization of this mutant.Ministerio de Educación de España por financiar la Ayuda del Programa de Formación de Profesorado Universitario (FPU, Referencia AP2009-2351

Identificación de nuevos genes implicados en la iniciación y desarrollo de las raíces laterales en "Arabidopsis thaliana"

Las plantas son organismos sésiles que deben crecer en el medio donde germinan y adaptarse a las condiciones ambientales que les rodean. El sistema radicular es fundamental para su adaptación y eficiencia reproductiva, ya que es el órgano encargado de adquirir nutrientes y agua del medio. El incremento del sistema radicular está determinado por el número y posición de las raíces laterales. Entender cómo las plantas reorganizan su sistema radicular, permitirá obtener plantas que requieran una menor aportación de recursos. En este trabajo se han identificado nuevos genes implicados en la formación y desarrollo de las raíces laterales en “Arabidopsis thaliana” utilizando una estrategia genómica y otra genética. Con la primera, mediante una estrategia de “separación celular” y genómica, se han identificado más de 600 genes relacionados con este proceso y se ha analizado la función de un grupo de ellos que se seleccionaron en base a su expresión tisular. Mediante una estrategia genética, se ha identificado un mutante recesivo, “srol1”, que presenta un sistema radicular reducido dependiente de la calidad de la luz, del cual se ha llevado a cabo su caracterización fenotípica y molecular.Plants are sessile organisms that have to adapt their growth to the environment where they are obligated to live. To have an optimal adaptation, the root system plays a key role, because acquires the nutrients and water needed for the efficient growth and development of the plants. The growth of the root system is based on the length of the main root and on the number and disposition of the lateral roots. Understanding how plants can adapt (re-organize) their root system will allow us to get plants that require less input of resources. In this project we have identified new genes that are implicated in the lateral root formation and development in “Arabidopsis thaliana”. Using an approach based on the combination of specific cell sorting and transcriptomic we have identified over 600 genes that are involved in the development of the root system. We have analyzed the function of a set of these genes selected on bases of their tissue expression. Using a genetic approach, we have identified a recessive mutant, “srol1”, that shows a reduced root development that is dependent on the quality of the light perceived by the root system. We have carried out a phenotypic and molecular characterization of this mutant.Ministerio de Educación de España por financiar la Ayuda del Programa de Formación de Profesorado Universitario (FPU, Referencia AP2009-2351

The emerging role of reactive oxygen species signaling during lateral root development

Overall root architecture is the combined result of primary and lateral root growth and is influenced by both intrinsic genetic programs and external signals. One of the main questions for root biologists is how plants control the number of lateral root primordia and their emergence through the main root. We recently identified S-phase kinase-associated protein2 (SKP2B) as a new early marker for lateral root development. Here, we took advantage of its specific expression pattern in Arabidopsis (Arabidopsis thaliana) in a cell-sorting and transcriptomic approach to generate a lateral root-specific cell sorting SKP2B data set that represents the endogenous genetic developmental program. We first validated this data set by showing that many of the identified genes have a function during root growth or lateral root development. Importantly, genes encoding peroxidases were highly represented in our data set. Thus, we next focused on this class of enzymes and showed, using genetic and chemical inhibitor studies, that peroxidase activity and reactive oxygen species signaling are specifically required during lateral root emergence but, intriguingly, not for primordium specification itself. © 2014 American Society of Plant Biologists. All rights reserved