75 research outputs found
Entanglement of trapped-ion qubits separated by 230 meters
We report on an elementary quantum network of two atomic ions separated by
230 m. The ions are trapped in different buildings and connected with 520(2) m
of optical fiber. At each network node, the electronic state of an ion is
entangled with the polarization state of a single cavity photon; subsequent to
interference of the photons at a beamsplitter, photon detection heralds
entanglement between the two ions. Fidelities of up to are
achieved with respect to a maximally entangled Bell state, with a success
probability of . We analyze the routes to improve these
metrics, paving the way for long-distance networks of entangled quantum
processors
Cell cycle-dependent acetylation of Rb2/p130 in NIH3T3 cells
The retinoblastoma protein (pRb) and the pRb-related proteins, p130 and p107, form the βpocket protein' family of cell cycle regulatory factors. A well characterized function of these proteins is the cell cycle-dependent regulation of E2F-responsive genes. The biological activity of pocket proteins is regulated by phosphorylation and for the founding member pRb it has been shown that acetylation also has an important role in modulating its function during the cell cycle. Here, we show that hyperphosphorylated retinoblastoma 2 (Rb2)/p130 also exists in an acetylated form in NIH3T3 cells. Acetylated p130 is present in the nucleus but not in the cytoplasm. Acetylation is cell cycle dependent, starting in S-phase and persisting until late G2-period. Using recombinant p130 and truncated forms for in vitro acetylation by the acetyltransferase p300, we could identify K1079 in the C-terminal part as the major acetylation site by mass spectrometry. Minor acetylation sites were pinpointed to K1068 and K1111 in the C-terminus, and K128 and K130 in the N-terminus. The human papilloma virus 16 protein-E7 preferentially binds to acetylated p130 and significantly increases in vitro p130 acetylation by p300
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A State-Dependent Quantification of Climate Sensitivity Based On Paleodata of the Last 2.1 Million Years
The evidence from both data and models indicates that specific equilibrium climate sensitivity S[X]βthe global annual mean surface temperature change (ΞTg) as a response to a change in radiative forcing X (ΞR[X])βis state dependent. Such a state dependency implies that the best fit in the scatterplot of ΞTg versus ΞR[X] is not a linear regression but can be some nonlinear or even nonsmooth function. While for the conventional linear case the slope (gradient) of the regression is correctly interpreted as the specific equilibrium climate sensitivity S[X], the interpretation is not straightforward in the nonlinear case. We here explain how such a state-dependent scatterplot needs to be interpreted and provide a theoretical understandingβor generalizationβhow to quantify S[X] in the nonlinear case. Finally, from data covering the last 2.1 Myr we show thatβdue to state dependencyβthe specific equilibrium climate sensitivity which considers radiative forcing of CO2 and land ice sheet (LI) albedo, math formula, is larger during interglacial states than during glacial conditions by more than a factor 2
Postglacial recolonizations, watershed crossings and human translocations shape the distribution of chub lineages around the Swiss Alps
The Effect of Recurrent Floods on Genetic Composition of Marble Trout Populations
A changing global climate can threaten the diversity of species and ecosystems. We explore the consequences of catastrophic disturbances in determining the evolutionary and demographic histories of secluded marble trout populations in Slovenian streams subjected to weather extremes, in particular recurrent flash floods and debris flows causing massive mortalities. Using microsatellite data, a pattern of extreme genetic differentiation was found among populations (global FST of 0.716), which exceeds the highest values reported in freshwater fish. All locations showed low levels of genetic diversity as evidenced by low heterozygosities and a mean of only 2 alleles per locus, with few or no rare alleles. Many loci showed a discontinuous allele distribution, with missing alleles across the allele size range, suggestive of a population contraction. Accordingly, bottleneck episodes were inferred for all samples with a reduction in population size of 3β4 orders of magnitude. The reduced level of genetic diversity observed in all populations implies a strong impact of genetic drift, and suggests that along with limited gene flow, genetic differentiation might have been exacerbated by recurrent mortalities likely caused by flash flood and debris flows. Due to its low evolutionary potential the species might fail to cope with an intensification and altered frequency of flash flood events predicted to occur with climate change
1A6/DRIM, a Novel t-UTP, Activates RNA Polymerase I Transcription and Promotes Cell Proliferation
BACKGROUND: Ribosome biogenesis is required for protein synthesis and cell proliferation. Ribosome subunits are assembled in the nucleolus following transcription of a 47S ribosome RNA precursor by RNA polymerase I and rRNA processing to produce mature 18S, 28S and 5.8S rRNAs. The 18S rRNA is incorporated into the ribosomal small subunit, whereas the 28S and 5.8S rRNAs are incorporated into the ribosomal large subunit. Pol I transcription and rRNA processing are coordinated processes and this coordination has been demonstrated to be mediated by a subset of U3 proteins known as t-UTPs. Up to date, five t-UTPs have been identified in humans but the mechanism(s) that function in the t-UTP(s) activation of Pol I remain unknown. In this study we have identified 1A6/DRIM, which was identified as UTP20 in our previous study, as a t-UTP. In the present study, we investigated the function and mechanism of 1A6/DRIM in Pol I transcription. METHODOLOGY/PRINCIPAL FINDINGS: Knockdown of 1A6/DRIM by siRNA resulted in a decreased 47S pre-rRNA level as determined by Northern blotting. Ectopic expression of 1A6/DRIM activated and knockdown of 1A6/DRIM inhibited the human rDNA promoter as evaluated with luciferase reporter. Chromatin immunoprecipitation (ChIP) experiments showed that 1A6/DRIM bound UBF and the rDNA promoter. Re-ChIP assay showed that 1A6/DRIM interacts with UBF at the rDNA promoter. Immunoprecipitation confirmed the interaction between 1A6/DRIM and the nucleolar acetyl-transferase hALP. It is of note that knockdown of 1A6/DRIM dramatically inhibited UBF acetylation. A finding of significance was that 1A6/DRIM depletion, as a kind of nucleolar stress, caused an increase in p53 level and inhibited cell proliferation by arresting cells at G1. CONCLUSIONS: We identify 1A6/DRIM as a novel t-UTP. Our results suggest that 1A6/DRIM activates Pol I transcription most likely by associating with both hALP and UBF and thereby affecting the acetylation of UBF
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Small global-mean cooling due to volcanic radiative forcing
In both the observational record and atmosphere-ocean general circulation model (AOGCM) simulations of the last βΌβΌ 150 years, short-lived negative radiative forcing due to volcanic aerosol, following explosive eruptions, causes sudden global-mean cooling of up to βΌβΌ 0.3 K. This is about five times smaller than expected from the transient climate response parameter (TCRP, K of global-mean surface air temperature change per W mβ2 of radiative forcing increase) evaluated under atmospheric CO2 concentration increasing at 1 % yrβ1. Using the step model (Good et al. in Geophys Res Lett 38:L01703, 2011. doi:10.β1029/β2010GL045208), we confirm the previous finding (Held et al. in J Clim 23:2418β2427, 2010. doi:10.β1175/β2009JCLI3466.β1) that the main reason for the discrepancy is the damping of the response to short-lived forcing by the thermal inertia of the upper ocean. Although the step model includes this effect, it still overestimates the volcanic cooling simulated by AOGCMs by about 60 %. We show that this remaining discrepancy can be explained by the magnitude of the volcanic forcing, which may be smaller in AOGCMs (by 30 % for the HadCM3 AOGCM) than in off-line calculations that do not account for rapid cloud adjustment, and the climate sensitivity parameter, which may be smaller than for increasing CO2 (40 % smaller than for 4 Γ CO2 in HadCM3)
A review of progress towards understanding the transient global mean surface temperature response to radiative perturbation
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