Evaluation of the effectiveness and mechanism of action of the Chang-Kang-Fang formula combined with bifid triple viable capsules on diarrhea-predominant irritable bowel syndrome

IntroductionThe Chang-Kang-Fang (CKF) formula, a traditional Chinese herbal formula, can decrease serotonin (5-HT) levels and treat irritable bowel syndrome (IBS). Probiotics have a better synergistic effect on diarrhea-predominant IBS (IBS-D) when combined with 5-HT3 receptor antagonists. The present study aimed to elucidate the efficacy and the mechanisms of action of the CKF formula combined with bifid triple viable capsules (PFK) against IBS-D.MethodsThe rat models of IBS-D were induced by gavage with senna decoction plus restraint stress. The CKF formula, PFK and their combination were administered to the rats. Their effects were evaluated based on general condition of the rats and the AWR score. The levels of 5-HT and fos protein in the colon and hippocampus were measured by immunohistochemistry. The levels of SP and VIP, as well as ZO-1 and occludin in the colon, were determined by enzyme-linked immunosorbent assay and immunohistochemistry. The intestinal microbiota in faeces was analyzed by 16S rRNA high-throughput sequencing.ResultsThe results showed that the oral CKF formula combined with PFK (CKF + PFK) could significantly relieve the symptoms of IBS-D, including elevating the weight rate and decreasing the AWR score. Compared with the MC group, administration of CKF + PFK significantly reduced the expression of fos in the colon and hippocampus and that of 5-HT, SP and VIP in the colon and increased the levels of 5-HT in the hippocampus and ZO-1 and occludin in the colon. The above indexes exhibited statistical significance in the CKF + PFK group relative to those in the other groups. Moreover, treatment with CKF + PFK improved the diversity of intestinal microbiota and the abundance of Firmicutes, Lachnospiraceae and Ruminococcaceae but decreased those of Bacteroidetes and Prevotellaceae.ConclusionsThe CKF formula combined with PFK may have a synergistic effect on IBS-D by slowing gastrointestinal motility, lowering visceral hypersensitivity, enhancing the intestinal barrier function and modulating the composition of intestinal microbiota

Self-healing mechanisms in smart protective coatings: A review

Self-healing coatings inspired by biological systems possess the ability to repair physical damage or recover functional performance with minimal or no intervention. This article provides a comprehensive and updated review on the advantages and limitations associated with common autonomous and non-autonomous self healing mechanisms in protective organic coatings used for anti-corrosion purposes. The autonomous healing mechanisms are often enabled by embedding polymerizable healing agents or corrosion inhibitors in the coating matrices. For non-autonomous mechanisms, the healing effects are induced by external heat or light stimuli, which trigger the chemical reactions or physical transitions necessary for bond formation or molecular chain movement

Preparation and water-oxygen corrosion behavior of Si/Mullite+BSAS/Yb2Si2O7 environmental barrier coating by atmospheric plasma spraying

The Si/Mullite+BSAS/Yb2Si2O7 tri-layer environmental barrier coating was prepared on the surface of the SiC substrate by atmospheric plasma spraying using solid-state sintering Yb2Si2O7 feedstock. Scanning electron microscope （SEM），energy dispersive spectrometer （EDS），X-ray diffraction analyzer （XRD） and nanoindentation testing machine etc were employed to study the microstructure，phase composition，and mechanical properties of the coating. The results indicate that the powder is composed of 83% Yb2Si2O7 phase and 17% Yb2Si2O7 phase，the porosity of the Yb2Si2O7 layer obtained by plasma spraying is （6.61±0.65）%，the bonding strength of the coating is （22.82±3.55）MPa and the fracture toughness of the coating is （1.98±0.12）MPa·m1/2. Furthermore，the water-oxygen corrosion test results of the coating at 1350 ℃ show that the monoclinic phase contents of the Yb2Si2O7 phase decrease first and then increase. The thermally grown oxide SiO2 formed by high-temperature oxidation of the silicon bonding layer is compatible with Mullite+BSAS，and interdiffusion between Mullite+BSAS and Yb2Si2O7 layers isn’t detected. Deterioration of the silicon layer is a major limiting factor in the lifetime of the coating

TP53RK Drives the Progression of Chronic Kidney Disease by Phosphorylating Birc5

Abstract Renal fibrosis is a common characteristic of various chronic kidney diseases (CKDs) driving the loss of renal function. During this pathological process, persistent injury to renal tubular epithelial cells and activation of fibroblasts chiefly determine the extent of renal fibrosis. In this study, the role of tumor protein 53 regulating kinase (TP53RK) in the pathogenesis of renal fibrosis and its underlying mechanisms is investigated. TP53RK is upregulated in fibrotic human and animal kidneys with a positive correlation to kidney dysfunction and fibrotic markers. Interestingly, specific deletion of TP53RK either in renal tubule or in fibroblasts in mice can mitigate renal fibrosis in CKD models. Mechanistic investigations reveal that TP53RK phosphorylates baculoviral IAP repeat containing 5 (Birc5) and facilitates its nuclear translocation; enhanced Birc5 displays a profibrotic effect possibly via activating PI3K/Akt and MAPK pathways. Moreover, pharmacologically inhibiting TP53RK and Birc5 using fusidic acid (an FDA‐approved antibiotic) and YM‐155(currently in clinical phase 2 trials) respectively both ameliorate kidney fibrosis. These findings demonstrate that activated TP53RK/Birc5 signaling in renal tubular cells and fibroblasts alters cellular phenotypes and drives CKD progression. A genetic or pharmacological blockade of this axis serves as a potential strategy for treating CKDs

Monitoring grassland degradation and restoration using a novel climate use efficiency (NCUE) index in the Tibetan Plateau, China

Grassland degradation is one of the most pressing challenges in natural environment and anthropogenic society. However, there is yet no effective approach for monitoring the spatio-temporal pattern of large-scale grassland degradation. In particular, the research on grassland changes in the harsh natural environment such as the Qinghai-Tibet Plateau is still in its infancy due to complexity, and it is extremely difficult for humans to reach these remote areas. The annual changes in the grassland biomass might be the results of climate fluctuations or grassland degradation. To test the hypothesis, the impact of inter-annual climate fluctuations needs to be considered when monitoring the grassland degradation based on spatio-temporal change of grassland biomass. In this paper, we propose a Novel Climate Use Efficiency index (NCUE) by considering rainfall, temperature, sunlight time, wind speed, surface temperature, accumulated temperature, time lag effect, light, temperature and water suitability and their coordination climatic factors that mainly affect vegetation growth comprehensively, to monitor grassland change suitable for cold and dry climate characteristics of the Qinghai-Tibet Plateau, and to reduce the effect of inter-annual variability of grassland productivity caused by climate fluctuation. As a consequence, grassland degradation monitoring could be more accurate and objective than existing ecological indicators. Our experiments show that the slope of NCUE over 31 years from 1982 to 2012 is 0.0028, showing a recovery trend in grassland. Degradation and restoration of grassland exist at the same time, and their proportions are 20.49% and 23.89%, respectively. By comparing with in-situ measurements in 2013 and 2009, 68% consistency was achieved with our prediction, and the 70% consistency is achieved by comparing with the positive and negative change trend of accumulated NDVI during the growing season. Moreover, the comparative analysis of land use/cover changes (LUCC) from 1990 to 2010 shows 69% of consistency. The ratio of the area of grassland significantly degradation and recovered predicted by NCUE change trend is 1.41% and 1.43%, respectively. It occupies a very small area of the study area. Yet, that predicted by NDVI change trend is 42.17% and 31.90%, respectively, and about 70% of the area is detected as drastic changes. It shows that NDVI is sensitive to climate fluctuations, while NCUE reduces the impact of climate fluctuations, reflecting change of grassland being affected by human activities and long-term climate change. The novel NCUE has great potential and utility to minify the impact of climate fluctuation and reflect grassland changes over space and time quantitatively. Such ecological index provides a new understanding of spatial and temporal patterns of grassland degradation in the Three River Headwaters Region (TRHR) at the same time

Observation of Wavelength Tuning in a Mode-Locked Figure-9 Fiber Laser

We demonstrate an all-PM Er-doped soliton mode-locked fiber oscillator based on the figure-9 configuration with a compact adjustable reflection-type non-reciprocal phase shifter. An analytical model based on the Jones matrix is established to simulate the wavelength tuning phenomenon. Experimentally, it is observed that the increase in pump power results in a significant redshift in the spectrum of output pulses. When the angle of the half-wave plate is rotated in one direction, the output spectrum is redshifted and then blueshifted successively. Good qualitative agreement is presented between the simulations and the experimental results. It is shown that the increase in pump power changes the nonlinear phase shift, which causes the redshift of the transmittance curves at the laser output port. In contrast, the rotation of wave plates not only changes the nonlinear phase shift difference, but also causes variations in linear phase bias and modulation depth. The changes in these parameters lead to the redshift and blueshift of the transmission curves, which enables wavelength tuning

Observation of Wavelength Tuning in a Mode-Locked Figure-9 Fiber Laser

We demonstrate an all-PM Er-doped soliton mode-locked fiber oscillator based on the figure-9 configuration with a compact adjustable reflection-type non-reciprocal phase shifter. An analytical model based on the Jones matrix is established to simulate the wavelength tuning phenomenon. Experimentally, it is observed that the increase in pump power results in a significant redshift in the spectrum of output pulses. When the angle of the half-wave plate is rotated in one direction, the output spectrum is redshifted and then blueshifted successively. Good qualitative agreement is presented between the simulations and the experimental results. It is shown that the increase in pump power changes the nonlinear phase shift, which causes the redshift of the transmittance curves at the laser output port. In contrast, the rotation of wave plates not only changes the nonlinear phase shift difference, but also causes variations in linear phase bias and modulation depth. The changes in these parameters lead to the redshift and blueshift of the transmission curves, which enables wavelength tuning.&nbsp

LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease

Abstract Mitochondria comprise the central metabolic hub of cells and their imbalance plays a pathogenic role in chronic kidney disease (CKD). Here, we studied Lon protease 1 (LONP1), a major mitochondrial protease, as its role in CKD pathogenesis is unclear. LONP1 expression was decreased in human patients and mice with CKD, and tubular‐specific Lonp1 overexpression mitigated renal injury and mitochondrial dysfunction in two different models of CKD, but these outcomes were aggravated by Lonp1 deletion. These results were confirmed in renal tubular epithelial cells in vitro. Mechanistically, LONP1 downregulation caused mitochondrial accumulation of the LONP1 substrate, 3‐hydroxy‐3‐methylglutaryl‐CoA synthase 2 (HMGCS2), which disrupted mitochondrial function and further accelerated CKD progression. Finally, computer‐aided virtual screening was performed, which identified a novel LONP1 activator. Pharmacologically, the LONP1 activator attenuated renal fibrosis and mitochondrial dysfunction. Collectively, these results imply that LONP1 is a promising therapeutic target for treating CKD

Ultrafast convergent power-balance model for Raman random fiber laser with half-open cavity

Este artículo contiene 11 páginas, 7 figuras, 1 tabla.The power-relevant features of Raman random fiber laser (RRFL), such as lasing threshold, slope efficiency, and power distribution, are among the most critical parameters to characterize its operation status. In this work, focusing on the power features of the half-open cavity RRFL, an ultrafast convergent power-balance model is proposed, which highlights the physical essence of the most common RRFL type and sharply reduces the computation workload. By transforming the time-consuming serial calculation to a parallel one, the calculation efficiency can be improved by more than 100 times. Particularly, for different point-mirror reflectivities and different fiber lengths, the input-output power curves and power distribution curves calculated by the present model match nicely with those of the conventional model, as well as with the experimental data. Moreover, through the present model the relationship between point-mirror reflectivity and laser threshold is analytically derived, and the way for improving RRFL’s slope efficiency is also provided with a lucid theoretical explanation.National Natural Science Foundation of China (61635005, 61731006); Sichuan Provincial Project for Outstanding Young Science and Technology Scholars (2020JDJQ0024); 111 project (B14039); Conselho Nacional de Desenvolvimento Científico e Tecnológico; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (APQ-0602-1.05/14, Grant 465.763/2014-6).Peer reviewe