1,934 research outputs found
Effects of Transplanting Bone Marrow Stromal Cells Transfected with CXCL13 on Fracture Healing of Diabetic Rats
Background/Aims: Diabetic fracture have poor treatment and serious complications. Therefore, how to treat diabetic fracture is receiving increasing attention. This study aimed to investigate the effects of transplanting CXCL13-stimulated bone marrow stromal cells (BMSCs) on the fracture healing in diabetic rats. Methods: In vitro, RT-PCR was employed to examine the expression of CXCL13 in BMSCs in high glucose environment. MTT assay and apoptosis assay were utilized to determine the effects of CXCL13 overexpression on the proliferation and apoptosis of BMSCs respectively. ALP staining was applied to detect the ALP activity. In vivo, CXCL13-stimulated BMSCs were transplanted into the fracture sites of diabetic rats. At the 1st week, 2nd weeks, 4th week and 6th week after the operation, bone mineral density (BMD) and callus area measurement, ELISA detection, and HE staining were performed to evaluate the fracture healing. Results: Low BMD and less area of callus in diabetic rats showed that the recovery after fracture was worse in diabetic rats than in non-diabetic rats. Meanwhile, the expression of CXCL13 in serum was lower in diabetic rats than in non-diabetic rats. Overexpression of CXCL13 promoted the proliferation of BMSCs in vitro high glucose environment. After BMSCs transfected with CXCL13 being transplanted into the fracture sites of diabetic rats, it was found that the fracture healing was enhanced and ALP expression in serum became higher. HE staining results further verified the effects of transplantation of BMSCs transfected with CXCL13 on fracture healing in diabetic rats. Conclusion: These finding indicated that CXCL13 may play a critical role in the process of fracture healing, which could provide a deeper insight into molecular targets for the fracture healing in diabetic people
The LAMOST Complete Spectroscopic Survey of Pointing Area (LaCoSSPAr) in the Southern Galactic Cap I. The Spectroscopic Redshift Catalog
We present a spectroscopic redshift catalog from the LAMOST Complete
Spectroscopic Survey of Pointing Area (LaCoSSPAr) in the Southern Galactic Cap
(SGC), which is designed to observe all sources (Galactic and extra-galactic)
by using repeating observations with a limiting magnitude of in
two fields. The project is mainly focusing on the completeness of
LAMOST ExtraGAlactic Surveys (LEGAS) in the SGC, the deficiencies of source
selection methods and the basic performance parameters of LAMOST telescope. In
both fields, more than 95% of galaxies have been observed. A post-processing
has been applied to LAMOST 1D spectrum to remove the majority of remaining sky
background residuals. More than 10,000 spectra have been visually inspected to
measure the redshift by using combinations of different emission/absorption
features with uncertainty of . In total, there are 1528
redshifts (623 absorption and 905 emission line galaxies) in Field A and 1570
redshifts (569 absorption and 1001 emission line galaxies) in Field B have been
measured. The results show that it is possible to derive redshift from low SNR
galaxies with our post-processing and visual inspection. Our analysis also
indicates that up to 1/4 of the input targets for a typical extra-galactic
spectroscopic survey might be unreliable. The multi-wavelength data analysis
shows that the majority of mid-infrared-detected absorption (91.3%) and
emission line galaxies (93.3%) can be well separated by an empirical criterion
of . Meanwhile, a fainter sequence paralleled to the main population
of galaxies has been witnessed both in / and /
diagrams, which could be the population of luminous dwarf galaxies but
contaminated by the edge-on/highly inclined galaxies ().Comment: 19 pages, 14 figures, 2 MRT, accepted by ApJ
Gaussian Boson Sampling with Pseudo-Photon-Number Resolving Detectors and Quantum Computational Advantage
We report new Gaussian boson sampling experiments with
pseudo-photon-number-resolving detection, which register up to 255 photon-click
events. We consider partial photon distinguishability and develop a more
complete model for characterization of the noisy Gaussian boson sampling. In
the quantum computational advantage regime, we use Bayesian tests and
correlation function analysis to validate the samples against all current
classical mockups. Estimating with the best classical algorithms to date,
generating a single ideal sample from the same distribution on the
supercomputer Frontier would take ~ 600 years using exact methods, whereas our
quantum computer, Jiuzhang 3.0, takes only 1.27 us to produce a sample.
Generating the hardest sample from the experiment using an exact algorithm
would take Frontier ~ 3.1*10^10 years.Comment: submitted on 10 Apri
Altered Behaviors and Impaired Synaptic Function in a Novel Rat Model With a Complete Shank3 Deletion
Mutations within the Shank3 gene, which encodes a key postsynaptic density (PSD) protein at glutamatergic synapses, contribute to the genetic etiology of defined autism spectrum disorders (ASDs), including Phelan-McDermid syndrome (PMS) and intellectual disabilities (ID). Although there are a series of genetic mouse models to study Shank3 gene in ASDs, there are few rat models with species-specific advantages. In this study, we established and characterized a novel rat model with a deletion spanning exons 11–21 of Shank3, leading to a complete loss of the major SHANK3 isoforms. Synaptic function and plasticity of Shank3-deficient rats were impaired detected by biochemical and electrophysiological analyses. Shank3-depleted rats showed impaired social memory but not impaired social interaction behaviors. In addition, impaired learning and memory, increased anxiety-like behavior, increased mechanical pain threshold and decreased thermal sensation were observed in Shank3-deficient rats. It is worth to note that Shank3-deficient rats had nearly normal levels of the endogenous social neurohormones oxytocin (OXT) and arginine-vasopressin (AVP). This new rat model will help to further investigate the etiology and assess potential therapeutic target and strategy for Shank3-related neurodevelopmental disorders
Prevalence of frailty and pre-frailty and related factors in older adults with cardio-cerebral vascular disease in China: a national cross-sectional study
ObjectiveFrailty increases adverse clinical outcomes in older patients with cardio-cerebral vascular disease (CCVD). The aim of this study was to investigate the prevalence of frailty and pre-frailty in older adults with CCVD in China and the factors associated with it.Research design and methodsIn this cross-sectional study, we used data from the fourth Sample Survey of Aged Population in Urban and Rural China. We used the frailty index for frailty and pre-frailty assessment, and the diagnosis of CCVD in older adults was self-reported.ResultsA total of 53,668 older patients with CCVD were enrolled in the study. The age-standardized prevalence of frailty and pre-frailty in older patients with CCVD was 22.6% (95% CI 22.3–23.0%) and 60.1% (95% CI 59.7–60.5%). Multinomial logistic regression analyses showed that being female, increasing age, rural residence, illiteracy, widowhood, ethnic minority, living alone, no health screening during the last year, hospitalization during the last year, difficult financial status, comorbid chronic conditions, and disability in activities of daily living were associated with frailty and pre-frailty in older patients with CCVD.ConclusionCCVD is strongly associated with frailty and pre-frailty in older Chinese people, and assessment of frailty should become routine in the management of older CCVD patients. Appropriate public health prevention strategies should be developed based on identified risk factors for frailty in older CCVD patients, which can help prevent, ameliorate or reverse the development of frailty in CCVD in the older population
TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways
Primer Sequences used for RT-qPCR (5â to 3â). (DOCX 13 kb
Genome-wide selection footprints and deleterious variations in young Asian allotetraploid rapeseed
Brassica napus (AACC, 2n=38) is an important oilseed crop grown worldwide. However, little is known about the population evolution of this species, the genomic difference between its major genetic groups, such as European and Asian rapeseed, and the impacts of historical large-scale introgression events on this young tetraploid. In this study, we reported the de novo assembly of the genome sequences of an Asian rapeseed (B. napus), Ningyou 7 and its four progenitors and compared these genomes with other available genomic data from diverse European and Asian cultivars. Our results showed that Asian rapeseed originally derived from European rapeseed but subsequently significantly diverged, with rapid genome differentiation after hybridization and intensive local selective breeding. The first historical introgression of B. rapa dramatically broadened the allelic pool but decreased the deleterious variations of Asian rapeseed. The second historical introgression of the double-low traits of European rapeseed (canola) has reshaped Asian rapeseed into two groups (double-low and double-high), accompanied by an increase in genetic load in the double-low group. This study demonstrates distinctive genomic footprints and deleterious SNP (Single Nucleotide Polymorphism) variants for local adaptation by recent intra- and interspecies introgression events and provides novel insights for understanding the rapid genome evolution of a young allopolyploid cro
A huge-amplitude white-light superflare on a L0 brown dwarf discovered by GWAC survey
White-light superflares from ultra cool stars are thought to be resulted from
magnetic reconnection, but the magnetic dynamics in a fully convective star is
not clear yet. In this paper, we report a stellar superflare detected with the
Ground Wide Angle Camera (GWAC), along with rapid follow-ups with the F60A,
Xinglong 2.16m and LCOGT telescopes. The effective temperature of the
counterpart is estimated to be K by the BT-Settl model,
corresponding to a spectral type of L0. The band light curve can be modeled
as a sum of three exponential decay components, where the impulsive component
contributes a fraction of 23\% of the total energy, while the gradual and the
shallower decay phases emit 42\% and 35\% of the total energy, respectively.
The strong and variable Balmer narrow emission lines indicate the large
amplitude flare is resulted from magnetic activity. The bolometric energy
released is about ergs, equivalent to an energy release in a
duration of 143.7 hours at its quiescent level. The amplitude of mag ( or mag), placing it one of the highest amplitudes of
any ultra cool star recorded with excellent temporal resolution. We argue that
a stellar flare with such rapidly decaying and huge amplitude at distances
greater than 1 kpc may be false positive in searching for counterparts of
catastrophic events such as gravitational wave events or gamma-ray bursts,
which are valuable in time-domain astronomy and should be given more attention.Comment: 9 pages, 5 figures, 1 table, MNRAS accepte
- …