Development of Angiostrongylus costaricensis Morera and Céspedes 1971 (Nematoda: Angiostrongylidae) larvae in the intermediate host Sarasinula marginata (Semper 1885) (Mollusca: Soleolifera)

Submitted by Gentil Jeorgina ([email protected]) on 2012-07-25T18:01:21Z No. of bitstreams: 1 Development of Angiostrongylus costaricensis Morera.pdf: 280158 bytes, checksum: 59bd8ebb5e952e9dfbd3a067674af930 (MD5)Made available in DSpace on 2012-07-25T18:01:21Z (GMT). No. of bitstreams: 1 Development of Angiostrongylus costaricensis Morera.pdf: 280158 bytes, checksum: 59bd8ebb5e952e9dfbd3a067674af930 (MD5) Previous issue date: 2008Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, Minas Gerais, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, Minas Gerais, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, BrasilIn life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. Some larvae, recovered from sedimentation, were submitted to selective staining after paraffin embedded or inclusion in JB-4 to study inner structures. As control, four slugs were used, two killed at the beginning of infection and the others at the end of the experiment. At day 2 post infection, larvae were motionless and thick, presenting initial retention of granules. At day 4, L2 were detected, persisting until 43 days post infection. Larvae L2 displayed a large amount of granules rich in lipids and carbohydrates through its overall body, with more accumulation at the medial third corresponding to the esophagus–intestine transition site. Lipid granules, the main energetic source, were located at the basal and apical regions of intestinal cells. Both L1 and L3 presented bilateral alae, which is also common in other nematodes. Transition forms between L2 to L3 molts were also observed

Achatina fulica Bowdich, 1822 (Mollusca: Gastropoda) como hospedeiro intermediário potencial do Angiostrongylus costaricensis Morera & Céspedes 1971

Submitted by Gentil Jeorgina ([email protected]) on 2011-12-15T16:36:16Z No. of bitstreams: 1 Sarasinula marginata (Semper, 1885) (Mollusca, Soleolifera) from Belo.pdf: 258040 bytes, checksum: 272b63333c784fd82f04cb52587d0ff9 (MD5)Made available in DSpace on 2011-12-15T16:36:16Z (GMT). No. of bitstreams: 1 Sarasinula marginata (Semper, 1885) (Mollusca, Soleolifera) from Belo.pdf: 258040 bytes, checksum: 272b63333c784fd82f04cb52587d0ff9 (MD5) Previous issue date: 2003Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Superintendência de Controle de Endemias, São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Pontifícia Universidade Católica de Minas Gerais, Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Exemplares de Achatina fulica foram experimentalmente infectados com larvas de Angiostrongylus costaricensis, agente etiológico da angiostrongiliase abdominal, mostrando-se susceptíveis ao parasita. Achatina fulica pode representar um risco para a urbanização da angiostrongilíase abdominal devido a sua alta prolificidade contínua dispersão e notável adaptação a ambientes urbanos.Samples of Achatina fulica were experimentally infected with Angiostrongylus costaricensis larvae, etiological agent of abdominal angiostrongyliasis, showing that A. fulica is susceptible to the parasite. Achatina fulica may be a risk to urbanization of abdominal angiostrongyliasis presumably due to its high proliferation, continuous dispersion and remarkable adaptation in several Brazilian towns

Angiostrongylus cantonensis (Nematode: Metastrongyloidea) in molluscs from harbour areas in Brazil

Submitted by Nuzia Santos ([email protected]) on 2014-03-07T18:15:59Z No. of bitstreams: 1 Angiostrongylus cantonensis .pdf: 445296 bytes, checksum: 3d1dc51f9309b61a8103d07822a4338b (MD5)Made available in DSpace on 2014-03-07T18:15:59Z (GMT). No. of bitstreams: 1 Angiostrongylus cantonensis .pdf: 445296 bytes, checksum: 3d1dc51f9309b61a8103d07822a4338b (MD5) Previous issue date: 2012Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilSwiss Tropical and Public Health Institute. Basel, Switzerland/University of Basel, Basel. SwitzerlandFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil /Pontifícia Universidade Católica de Minas Gerais. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilAngiostrongylus cantonensis is the most common aetiological agent of human eosinophilic meningoencephalitis. Following a report indicating the presence of this parasite in Brazil in 2007, the present study was undertaken to investigate the presence of A. cantonensis in the surrounding Brazilian port areas. In total, 30 ports were investigated and the following molluscs were identified: Achatina fulica, Belocaulus sp., Bradybaena similaris sp., Cyclodontina sp., Helix sp., Leptinaria sp., Melampus sp., Melanoides tuberculata, Phyllocaulis sp., Pomacea sp., Pseudoxychona sp., Rhinus sp., Sarasinula marginata, Streptaxis sp., Subulina octona, Succinea sp., Tomigerus sp., Wayampia sp. and specimens belonging to Limacidae and Orthalicinae. Digestion and sedimentation processes were performed and the sediments were examined. DNA was extracted from the obtained larvae and the internal transcribed spacer region 2 was analysed by polymerase chain reaction-restriction fragment length polymorphism after digestion with the endonuclease ClaI. Of the 30 ports investigated in this study, 11 contained molluscs infected with A. cantonensis larvae. The set of infected species consisted of S. octona, S. marginata, A. fulica and B. similaris. A total of 36.6% of the investigated ports were positive for A. cantonensis, indicating a wide distribution of this worm. It remains uncertain when and how A. cantonensis was introduced into South America

Ocorrência de moluscos do gênero Biomphalaria em parques da cidade de Belo Horizonte, Minas Gerais, Brasil

Submitted by Nuzia Santos ([email protected]) on 2014-06-02T13:56:42Z No. of bitstreams: 1 21705-91536-2-PB.pdf: 656858 bytes, checksum: cff95f6a83797eaa2e69a0d261c7a2af (MD5)Made available in DSpace on 2014-06-02T13:56:42Z (GMT). No. of bitstreams: 1 21705-91536-2-PB.pdf: 656858 bytes, checksum: cff95f6a83797eaa2e69a0d261c7a2af (MD5) Previous issue date: 2012Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica.Swiss Tropical and Public Health Institute. Department of Epidemiology and Public Health. Basel, SwitzerlandFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintologia e Malacologia Médica.Até pouco tempo a esquistossomose era considerada a mais prevalente endemia entre a população rural de baixa renda, especialmente nos estados do Nordeste e Sudeste do Brasil, sendo por isso classificada como “endemia rural”. Estudos recentes têm demonstrado que o perfil epidemiológico desta doença está mudando, uma vez que vem sendo transmitida nas periferias e mesmo dentro de grandes centros urbanos. A cidade de Belo Horizonte-MG tem 71 parques, dos quais 55 são abertos à visitação pública. Em 31 (43,6%) deles há uma ou mais coleções hídricas. O objetivo deste trabalho foi investigar a presença de moluscos de importância médica nas coleções hídricas destes 31 parques da capital mineira. Foram coletados, em 11 parques, 551 exemplares de Biomphalaria, hospedeira intermediária do Schistosoma mansoni. Foram também coletados exemplares dos gêneros Drepanotremasp, Pomacea sp,Melanoides sp, Physa sp eLymnaea sp, este último hospedeiro intermediário de outro parasito causador da fasciolose.Todos os exemplares se mostraram negativos para cercárias de S. mansoni. No entanto, considerando a possível contaminação do meio ambiente com fezes humanas infectadas com ovos de S. mansoni e a presença dos hospedeiros intermediários, estes achados servem de alerta para uma possível instalação do ciclo de transmissão da esquistossomose em parques municipais da cidade de Belo Horizonte

Diversity of Freshwater Mollusks from Lake Pampulha, Municipality of Belo Horizonte, Minas Gerais, Brazil

The artificially created Lake Pampulha, within the metropolitan area of Belo Horizonte, Minas Gerais State, lacks detailed information about its freshwater mollusks, representing a challenge for the assessment and conservation of this ecosystem. In this study, conducted during June and August 2021, we collected specimens on four different occasions and at five sampling points around the lake, using different sampling equipment, such as a shovel and a scoop. During these collections, we identified, enumerated and measured 1538 species of mollusks and additionally examined the presence of trematode larvae. We identified twelve species of fresh water bivalves and gastropods: Biomphalaria straminea, Biomphalaria kuhniana, Biomphalaria occidentalis, Drepanotrema cimex, Pomacea maculata, Stenophysa marmorata, Physa acuta, Gundlachia ticaga, Melanoides tuberculata, Pseudosuccinea columella, Omalonyx matheroni and Corbicula largillierti. Echinostome and strigeocercaria types of larval trematodes were detected in B. straminea. Notably, some species of mollusks have not previously been recorded at Lake Pampulha. The analyses revealed differences in the composition and abundance of species, highlighting the higher number of mollusk species in areas more impacted by human actions. This study expands our understanding of mollusk diversity at Lake Pampulha, and provides valuable data for longitudinal comparisons of water quality and considerations of the conservation of native species. Furthermore, it highlights the importance of choosing appropriate sampling equipment, depending on the research objectives. The presence of invasive species of medical and veterinary relevance as intermediate hosts of parasites reinforces the need for efficient environmental protection strategies to preserve this artificial, aquatic environment widely used by the local population and by tourists

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Submitted by Gentil Jeorgina ([email protected]) on 2012-07-18T16:06:18Z No. of bitstreams: 1 Angiostrongylus costaricensis.pdf: 590330 bytes, checksum: a81269352bec17c7fda8d7fb562ffd8e (MD5)Made available in DSpace on 2012-07-18T16:06:18Z (GMT). No. of bitstreams: 1 Angiostrongylus costaricensis.pdf: 590330 bytes, checksum: a81269352bec17c7fda8d7fb562ffd8e (MD5) Previous issue date: 2008Universidade Federal de Minas Gerais. Departamento de Zoologia. Laboratório de Malacologia e Sistemática Molecular. Belo Horizonte, Minas Gerais, Brasil.Universidade Federal de Minas Gerais. Departamento de Zoologia. Laboratório de Malacologia e Sistemática Molecular. Belo Horizonte, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, Minas Gerais, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, Minas Gerais, Brasil.Universidade Federal de Minas Gerais. Departamento de Zoologia. Laboratório de Malacologia e Sistemática Molecular. Belo Horizonte, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with stage 1 larvae. Obtained stage 3 larvae were orally inoculated in mice, and after 30 days, adult worms and stage 1 larvae were recovered, demonstrating Omalonyx susceptibility and suitability to infection. To define the parasite migration routes, specimens of Omalonyx with 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 2 days, 5 days, 10 days, 12 days, 15 days, 20 days, 21 days, 25 days, 28 days, and 30 days of infection were fixed and serially sectioned. Histological sections were stained with hematoxylin-eosin. The results were compared to those described in S. marginata. Oral and cutaneous infections were noted. After the penetration, larvae were retained, mainly in the fibromuscular tissue, by hemocytes, or they spread to the whole organism through the circulation, following the anatomical structure of the vasculature. The perilarval hemocyte reaction in Omalonyx was more intense until stage 2 larva instar, decreasing in the presence of stage 3 larvae. Differences in some aspects of hemocyte reaction between S. marginata and Omalonyx exemplify interspecific peculiarities in snail response to the same parasite

Distribuição geográfica dos hospedeiros intermediários do Schistosoma mansoni nos estados do Paraná, Minas Gerais, Bahia, Pernambuco e Rio Grande do Norte, 2012-2014

Resumo Objetivo: descrever a distribuição geográfica dos hospedeiros intermediários do Schistosoma mansoni em cinco estados brasileiros. Métodos: estudo transversal; foram selecionados municípios dos estados do Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) e Rio Grande do Norte (98), nos anos de 2012 a 2014; esses municípios foram escolhidos por não possuírem registros atualizados da presença de caramujos hospedeiros intermediários de S. mansoni; moluscos foram capturados, taxonomicamente identificados e examinados para verificação de cercárias de S. mansoni. Resultados: os trabalhos foram realizados em 427 municípios (99,5% dos 429 selecionados); foi registrada presença de moluscos em 300 (70,2%) municípios e a ocorrência de Biomphalaria glabrata em 62 (21%) municípios, B. straminea em 181 (60%), B. tenagophila em três (1%); associação de B. glabrata/B. straminea foi encontrada em 53 (18%), e de B. glabrata/B. tenagophila em um (0,3%). Conclusão: os registros de B. glabrata, B. straminea e B. tenagophila estão de acordo com a distribuição conhecida