Chromobacterium Violaceum: A Review Of Pharmacological And Industiral Perspectives

Violet-pigmented bacteria, which have been described since the end of the 19th century, are occasionally the causative agent of septicemia and sometimes cause fatal infection in human and animals. Bacteria, producing violet colonies due to the production of a nondiffusible pigment violacein, were classified as a redefined genus Chromobacterium. Chromobacterium violaceum is Gram-negative, and saprophyte from soil and water is normally considered nonpathogenic to human, but is an opportunistic pathogen of extreme virulence for human and animals. The biosynthesis and biological activities of violacein and the diverse effects of this pigment have been studied. Besides violacein, C. violaceum produces other antibiotics, such as aerocyanidin and aerocavin, which exhibit in vitro activity against both Gram-negative and Gram-positive bacteria. 3,6-Dihydroxyindoxazene and Y-TO678h exhibit a selective activity against Gram-negative bacteria. Arphamenine A and B, and FR901228, that enhanced immunoresponse, and potentiators of β-lactam antibiotics and chelators such as ferrioxamine exhibit important clinical potential applications. Lipopolysaccharides and polyhydroxyesters together with several enzymes appear as important metabolites with biotechnological applications. Many of these metabolites were already studied at the genome level.273201222Aldridge, K.E., Valainis, G.T., Sanders, C.V., Comparison of the in vitro of citoprofloxacin and 24 other antimicrobial agents against clinical strains of Chromobacterium violaceum (1988) Diagnostic Microbiol. Infect. Dis., 10, pp. 31-39Antônio, R.V., (1994) Violacein biosynthesis by Chromobacterium violaceum: Synthesis and biological activities of a probable intermediate, , (Durán, N. and Haun, M. Supervisors), Ph.D. Dissertation, Biology Institute, Universidade Estadual de Campinas, BrazilAudebaud, G., Ganzin, M., Ceccaldi, J., Merveille, P., Isolement dun Chromobacterium violaceum a partir de lessions hepatiques observees chez un singe Cercopithecus cephus etude et pouvoir pathogene (1954) Ann. L'Institute Pasteur., 87, pp. 413-417August, P.R., Grossman, T.H., Minor, C., Draper, M.P., MacNeil, I.A., Pemberton, J.M., Call, K.M., Osburne, M.S., Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum (2000) J. Mol. Microbiol. Biotechnol., 2, pp. 513-519August, P.R., Grossman, T.H., Minor, C., Draper, M.P., MacNeil, I.A., Pemberton, J.M., Call, K.M., Osburne, M.S., Hydroxylases VioABCD in Chromobacterium violaceum (2000) NCBI-Protein, , AAD51809-51811Bachofen, R., Schenk, A., Quorum sensing autoinducers: Do they play a role in natural microbial habitats? (1998) Microbiol. Res., 153, pp. 61-63Ballantine, J.A., Beer, R.J.S., Crutchley, D.J., Dodd, G.M., Palmer, D.R., The synthesis of violacein and related compounds (1958) J. Chem. Soc., pp. 232-233Ballantine, J.A., Beer, R.J.S., Crutchley, D.J., Dodd, G.M., Palmer, D.R., The chemistry of bacteria. Part VIII. The synthesis of violacein and related compounds (1960) J. Chem. Soc., pp. 2292-2299Balows, A., Truper, H.G., Dworkin, M., Harder, W., Schleifer, K.H., (1992) The prokaryots, 2nd. ed., 3. , Springer-Verlag, N.YBazylinski, D.A., Palome, E., Blakemore, N.A., Blakemore, R.P., Denitrification by Chromobacterium violaceum (1986) Appl. Environ. Microbiol., 52, pp. 696-699Beinert, H., Copper in biological system. A report from 6th. Manziana Conference (1991) J. Inorg. Biochem., 44, pp. 173-218Lee, J., Kim, J.S., Nahm, C.H., Choi, J.W., Kim, J., Pai, S.H., Moon, K.H., Chong, Y., Two cases of Chromobacterium violaceum infection after injury in a subtropical region (1999) J. Clin. Microbiol., 37, pp. 2068-2070Letendre, C.H., Dickens, G., Guroff, G., Tryptophan hydroxylase of Chromobacterium violaceum (1974) J. Biol. Chem., 249, pp. 7186-7191Li, K.W., Wu, J., Xing, W.N., Simon, J.A., Total synthesis of the antitumor depsipeptide FR-901,228 (1996) J. Am. Chem. Soc., 118, pp. 7237-7238Lichstein, H.C., Van de Sand, V.F., Violacein an antibiotic pigment produced by Chromobacterium violaceum (1945) J. Infect. Dis., 76, pp. 47-51Liebergesell, M., Steinbuchel, A., Steinbuechel, A., Production of poly:hydroxyalkanoate polymer(s) - by culturing Chromatium vinosum or transformants containing C. vinosum PHA biosynthetic genes, Eur. Patent. WO 9302194-A1, 1993Liu, W.C., New aerocavin antibiotic - Is (3E,6E,9E,12 alpha, 14 beta)-12-hydroxy-14-(2-hydroxyundecyl)-10-methyl-2-oxo: oxa:cyclo:tetra:deca-3,6,9-tri:ene-4-acetic acid, US Patent US4686299-A, 1987Liu, C.H., Chu, R.M., Weng, C.N., Lin, Y.L., Chi, C.S., An acute pleuropneumpnia in a pig caused by Chromobacterium violaceum (1989) J. Compar. Pathol., 100, pp. 459-463Macadam, A.M., Knowles, C.J., Purification and properties of β-cyano-L-alanine synthase from the cyanide producing bacterium Chromobacterium violaceum (1984) Biochim. Biophys. Acta., 786, pp. 123-132Macher, A.M., Casale, T.B., Fauci, A.S., Chronic granulomatous disease of childhood and Chromobacterium violaceum infections in the southeastern (1982) Ann. Intern. Med., 97, pp. 51-55Macher, A.M., Casale, T.B., Gallin, J.I., Boltansky, H., Fauci, A.S., Chromobacterium violaceum infectious and chronic granulomatous disease (1983) Ann. Intern. Med., 98, pp. 259-259Machin-Villafranca, C., Ley, N., Torres-Hernandez, L., Infección por Chromobacterium violaceum. Presentación de un caso com evolución fatal (1986) Rev. Cubana Med. Trop., 38, pp. 353-357Martin, J., Brimacombe, J., Chromobacterium violaceum septicemia- The intensive care management of 2 cases (1992) Anaethes. Intensive Care, 20, pp. 88-90Bergonzini, C., Um nuevo bacterio colorato (1881) Ann. Soc. Natural. Modena. Ser. 2, 14, pp. 149-158May, G., Brummer, B., Ott, H., Purification of the antiviral compound violacein from cultures of Chromobacterium (1991) Ger. Offen., , DE 3935066Melo, P.S., (1996) Pigments from Chromobacterium violaceum and Serratia marcescens, , (Haun, M, e Durán, N., Supervisors), M.Sc. Thesis, Institute of Biology, Universidade Estadual de Campinas, BrazilMelo, P.S., Haun, M., Durán, N., In vitro evaluation of violacein toxicity in different cell lines (1996) Proc. VII Panamerican Associastion of Biochemistry and Molecular Biology-PABMB, , ChileMelo, P.S., Maria, S.S., Vidal, B.C., Haun, M., Durán, N., Violacein cytotoxicity and induction of apoptosis in V79 cells (2000) In vitro Cell. Dev. Biol. Animal, 36, pp. 639-543Mergaert, J., Schirmer, A., Hauben, L., Mau, M., Hoste, B., Kersters, K., Jendrossek, D., Swings, J., Isolation and identification of poly(3-hydroxyvalerate)-degrading strains of Pseudomonas lemoignei (1996) Intern. J. Syst. Bacteriol., 46, pp. 769-773McClean, K.H., Winson, M.K., Fish, L., Taylor, A., Chhabra, S.R., Camara, M., Daykin, M., Williams, P., Quorum sensing and Chromobacterium violaceum: Exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones (1997) Microbiology-UK, 143, pp. 3703-3711McClure, H.M., Chang, J., Chromobacterium violaceum infection in a nonhuman primate (Macaca assamensis) (1976) Lab. Animal Sci., 26, pp. 807-810Midani, S., Rathore, M., Chromobacterium violaceum infection (1998) Southern Med. J., 91, pp. 464-466Miller, D.P., Blevins, W.T., Steele, D.B., Stower, M.D., A comparative study of virulent and avirulent strains of Chromobacterium violaceum (1988) Can. J. Microbiol., 34, pp. 249-255Momen, A.Z.M.R., Hoshino, T., Biosynthesis of violacein: Intact incorporation of the tryptophan molecule on the oxindole side, with intramolecular rearrangement of the indole ring on the 5-hydroxyindole side (2000) Biosci. Biotechnol. Biochem., 64, pp. 539-549Blereau, R.P., Septicemia and death caused by Chromobacterium violaceum (1980) Southern Med. J., 73, pp. 1093-1094Momen, A.Z.M.R., Mizuoka, T., Hoshino, T., Studies on the biosynthesis of violacein. Part 9. Green pigments possessing tetraindole and dipyrromethene moieties, chromoviridans and deoxychromoviridans, produced by a cell-free extract of Chromobacterium violaceum and their biosynthetic origins (1998) J. Chem. Soc. Perkin Trans. 1, pp. 3087-3092Mueller, A., Zaehner, H., Metabolic products of microroganisms LXV. Ferrioxamines for Eubacteriales (1968) Arch. Microbiol., 62, pp. 257-260Murata, M., Towatari, M., Kosugi, H., Tanimoto, M., Ueda, R., Saito, H., Naoe, T., Apoptotic cytotoxic effects of a histone deacetylase inhibitors, FK228, on malignant lymphoid cells (2000) Jpn. J. Cancer Res., 91, pp. 1154-1160Nakajima, H., Kim, Y.B., Terano, H., Yoshida, M., Horinouchi, S., FR901228, a potent antitumor antibiotic, is a novel histone deacetylase inhibitor (1998) Experiment. Cell Res., 241, pp. 126-133Nakata, H., Yamauchi, T., Fujisawa, H., Phenylalanine hydroxylase from Chromobacterium violaceum, purification and characterization (1979) J. Biol. Chem., 254, pp. 1829-1833Ognibene, A.J., Thomas, E., Fatal infection due to Chromobacterium violaceum in Vietnam (1970) Am. J. Clin. Pathol., 54, pp. 607-612Ohuchi, S., Okuyama, A., Naganawa, H., Kawamura, K., Aoyagi, T., Umezawa, H., Isolation of two intermediates of arphamenine B biosynthesis (1997) Chem. Abstr., 106, pp. 172-650. , Meiji Seika Kenkyu Nenpo. 24, 44-47, 1985Onile, A., Sobowale, B.O., Odugbemi, T., Human infection due to Chromobacterium violaceum. A report from Ilorin, Nigeria (1984) East Afr. Med. J., 61, pp. 849-852Onishi, A., Liotta, L.J., Benkovic, S.J., Cloning and expression of Chromobacterium violaceum phenylalanine hydroxylase in Escherichia coli and comparison of amino acid sequence with mammalian aromatic amino acid hydroxylases (1991) J. Biol. Chem., 266, pp. 18454-18459Onishi, A., Liotta, L.J., Benkovic, S.J., Chromobacterium violaceum phenylalanine hydroxylase gene, complete cds (1991) NCBI-Protein, , P30967Bilton, B.D., Johnson, L.W., Recurrent nonfatal Chromobacterium violaceum infection in a nonimmunocompromised patient (2000) Infect. Med., 17, pp. 686-690A40996AAA23115M55915Parker, W.L., Rathnum, M.L., Johnson, J.H., Wells, J.S., Principe, P.A., Sykes, R.B., Aerocyanidin, a new antibiotic produced by Chromobacterium violaceun (1988) J. Antibiotics, 41, pp. 454-460Pember, S.O., Johnson, K.A., Villafranca, J.J., Benkovic, S.J., Mechanistic studies on phenylalanine hydroxylase from Chromobacterium vlaceum. Evidence for the formation of an enzyme-oxygen complex (1989) Biochemistry, 28, pp. 2124-2130Pember, S.O., Villafranca, J.J., Benkovic, S.J., Chromobacterium violaceum phenylalanine 4-monooxygenase (1987) Methods Enzymol., 142, pp. 50-56Perberton, J.M., Vincent, K.M., Penfold, R.J., Cloning and heterologous expression of the violacein biosynthesis gene cluster from Chromobacterium violaceum (1991) Curr. Microbiol., 22, pp. 355-358Petrillo, V.F., Severo, V., Santos, M.M., Edelweiss, E.L., Recurrent infection with Chromobacterium violaceum: First case report from South America (1984) J. Infect., 9, pp. 167-169Ponte, R., Jenkins, S.G., Fatal Chromobacterium violaceum infections associated with exposure to stagnant water (1992) Pediatr. Infect. Dis., 11, pp. 583-586Reilly, J., Pyne, G., CXL. On the pigment produced by Chromobacterium violaceum (1927) Biochem. J., 21, pp. 1059-1064Blosser, R.S., Gray, K.M., Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers (2000) J. Microbiol. Methods, 40, pp. 47-55Ressler, C., Abe, O., Kondo, Y., Cottrell, B., Abe, K., Purification and characterization from Chromobacterium violaceum of an enzyme catalyzing synthesis of γ-cyanoα-aminobutyric acid and thiocyanate (1973) Biochemistry, 12, pp. 5369-5377Rettori, D., (1996) Production, extraction and purification of violacein: An antibiotic produced by Chromobacterium violaceum, , (Durán, N., Supervisor), M.Sc. Thesis, Chemistry Institute, Universidade Estadual de Campinas, BrazilRettori, D., (2000) Physico-chemical and biological studies of violacein: A pigment produced by Chromobacterium violaceum, , (Durán, N., Supervisor), Ph.D. Dissertation, Chemistry Institute, Universidade Estadual de Campinas, BrazilRettori, D., Durán, N., Stationary tray bioreactor for bacterial metabolites production, Brazilian Patent, PIBr 9702986-6, 1997Rettori, D., Durán, N., Production, extraction and purification of violacein: An antibiotic produced by Chromobacterium violaceum (1998) World. J. Microbiol. Biotechnol., 14, pp. 685-688Richards, C., Chromobacterium violaceum, opportunistic pathogenic bacteria isolated in tropical and subtropical areas (1993) Bull. Soc. Pathol. Exotique, 86, pp. 169-173Richards, C., Chromobacterium violaceum, opportunistic pathogenic bacteria isolated in tropical and subtropical areas (1993) Bull. Soc. Pathol. Exotique, 86, pp. 307-307Riveros, R., (1986) Chemistry and biological activity of 1,3-dihydro-2H-indole-2-one derivatives, , (Durán, N., Supervisor), M.Sc. Thesis, Chemistry Institute, Universidade Estadual de Campinas, BrazilRiveros, R., (1989) Bacterial chemistry: Biosynthesis and biological activity of Chromobacterium violaceum metabolites, , (Durán, N., Orientador), Ph.D. Dissertation, Chemistry Institute, Universidade Estadual de CampinasRiveros, R., Haun, M., Campos, V., Durán, N., Bacterial chemistry-IV: Total characterization of violacein. An antibiotic trypanocide from Chromobacterium violaceum (1988) Arq. Biol. Tecnol., 31, pp. 475-487Riveros, R., Haun, M., Durán, N., Bacterial chemistry-V: Effect of growth conditions on production of violascein by Chromobacterium violaceum BB-78 strain (1989) Brazilian J. Med. Biol. Res., 22, pp. 569-577Boisbaudran, L., Matière colorante se formant dans la colle de farine (1882) Comp. Rend. Acad. Sci., 94, pp. 562-563Roberts, S.A., Morris, A.J., McIvor, N., EllisPegler, R., Chromobacterium violaceum infection of the deep neck tissues in a traveler to Thailand (1997) Clin. Infect. Dis., 25, pp. 334-335Sandor, V., Senderowicz, A., Mertins, S., Sackett, D., Sausville, E., Blagosklonny, M.V., Bates, S.E., P21-dependent G(1) arrest with downregulation of cyclin D1 and upregulation od cyclin E by the histone deacetylase inhibitor FR901228 (2000) Br. J. Cancer, 83, pp. 817-825Sandor, V., Robbins, A.R., Robey, R., Myers, T., Sauville, E., Bates, S.E., Sackett, D.L., FR901228 causes mitotic arrest but does not alter microtubule polymerization (2000) Anti-Cancer Drugs, 11, pp. 445-454Shigematsu, N., Ueda, H., Takase, S., Tanaka, H., Yamamoto, K., Tada, T., FR901228, a novel antitumor bicyclic depsipeptide produced by Chromobacterium violaceum N°-968.2. Structure determination (1994) J. Antibiotics, 47, pp. 311-314Shirata, A., Tsukamoto, T., Yasui, H., Hata, T., Hayasaka, S., Kojima, A., Kato, H., Isolation of bacteria producing bluish-purple pigment and use for dyeing (2000) Japan Agric. Res. Quart., 34, pp. 131-140Shirazi-Beechey, S.P., Sraya, P., Knowles, C.J., Serine hydroxymethyltransferase and glycine cleavage enzyme from the cyanogenic bacterium Chromobacterium violaceum (1984) J. Gen. Microbiol., 130, pp. 521-525Sherwood, M., Bacterial plastic comes to market (1983) Bio/Technology., 1, pp. 388-389Simo, F., Reuman, P.D., Martinez, F.J., Ayoub, E.M., Chromobacterium violaceum as a cause of periorbital cellulitis (1984) Pediatr. Infect. Dis. J., 3, pp. 561-563Singh, P.D., Liu, W.C., Gougoutas, J.Z., Malley, M.F., Porubcan, M.A., Trejo, W.H., Wells, J.S., Sykes, R.B., Aerocavin, a new antibiotic produced by Chromobacterium violaceum (1988) J. Antibiotics, 41, pp. 446-453Sippel, W.L., Medina, G., Atwood, M.B., Outbreaks of disease in animals associated with Chromobacterium violaceum. 1. The disease in swine (1954) I. Am. Veter. Med. Assoc., 124, pp. 467-471Bromberg, N., (2000) Violacein biotransformations, , (Durán, N., Supervisor). M.Sc. Thesis, Chemistry Institute, Universidade Estadual de Campinas, BrazilSivendran, R., Tan, S.H., Pathogenicity of nonpigmented cultures of Chromobacterium violaceum (1977) J. Clin. Microbiol., 5, pp. 514-516Smith, A.D., Hunt, R.J., Solubilization of gold by Chromobacterium violaceum (1985) J. Chem. Technol. Biotechnol. B. Biotechnol., 35, pp. 110-116Sneath, P.H.A., Whelan, J.P.F., Singh, R.B., Edwards, D., Fatal infection by Chromobacterium violaceum (1953) Lancet, 265, pp. 276-277Snowe, R.J., Chromobacrterium violaceum, pathogenic for man (1971) J. Infect. Dis., 123, pp. 226-229Sorensen, I.R.U., Jacobs, M.R., Shurin, S.B., Chromobacterium violaceum adenitis acquired in the northern United States as complication if chronic granulomatous disease (1985) Pediat. Infect. Dis. J., 4, pp. 701-702Starr, A.J., Cribbett, L.S., Poklepovic, J., Freidman, H., Ruffolo, E.H., Chromobacterium violaceum presenting as a surgical emergency (1981) Southern Med. J., 74, pp. 1137-1139Stenbuchel, A., Debzi, E.M., Marchessault, R.H., Timm, A., Synthesis and production of poly(3-hydroxyvaleric acid) homopolyester by Chromobacterium violaceum (1993) Appl. Microbiol. Biotechnol., 39, pp. 443-449Steibuchel, A., Schmack, G., Large-scale production of poly (3-hydrovaleric acid) by fermentation of Chromobacterium violaceum, processing, and characterization of the homopolyester (1995) J. Environ. Polym. Degrad., 3, pp. 243-258Ti, T.Y., Tan, W.C., Chong, A.P.Y., Lee, E.H., Nonfatal and fatal infections caused by Chromobacterium violaceum (1993) Clin. Infect. Dis., 17, pp. 505-507Tobie, W.C., The pigment of Bacillus violaceus I. The production, extraction, and purification of violacein (1934) J. Bacteriol., 29, pp. 223-227Buchanan, E., Studies in the nomenclature and classification of the bacteria (1918) J. Bacteriol., 3, pp. 52-57Tucker, R.E., Winter, W.G., Wilson, H.D., Osteomyelitis associated with Chromobacterium violaceum sepsis-case-report (1979) J. Bone Joint Sur. Am., 61, pp. 949-951Turner, S.J., Lewis, G.D., Saul, D.J., Baker, C.S., Rodrigo, A.G., Chromobacterium violaceum 16S ribosomal RNA gene, partial sequence spanning the V3-V4 regions (1996) NCBI-Nucleotide, , U65966Ueda, H., Nakajima, H., Hori, Y., Fujita, T., Nishimura, M., Goto, T., Okuhara, M., FR901228, a novel antitumor bicyclic depsipeptide produced by Chromobacterium violaceum N°968. 1. Taxonomy, fermentation, isolation, physicochemical and biologiByrd, J.C., Shinn, C., Ravi, R., Willis, C.R., Waselenko, J.K., Flinn, I.W., Dawson, N.A., Grever, M.R., Depsipeptide (FR901228): A novel therapeutic agent with selective, in vitro activity against human B-cell chronic lymphocytic leukemia cells (1999) Blood, 94, pp. 1401-1408Byrd, J.C., Waselenko, J.K., Keating, M., Rai, K., Grever, M.R., Novel therapies for chronic lymphocytic leukemia in the 21st century (2000) Seminar Oncol., 27, pp. 587-597Caldas, L.R., Photochemistry and photobiology in a virgin land (1977) Photochem. Photobiol., 26, pp. 1-2Caldas, L.R., Um pigmento nas águas negras (1990) Ciência Hoje, 11, pp. 56-57Caldas, L.R., Leitao, A.A.C., Santos, S.M., Tyrrell, R.M., Preliminary experiments on the photobiological properties of violacein (1978) Intern. Symp. Curr. Topics Radiol. Photobiol. Acad. Brasil. Cienc., pp. 121-132. , Rio de JaneiroCarrasco, L., Astorga, R., Mendez, A., Maldonado, A., Barazona, J., Perea, A., Acute pleuropneumonia in Barbary sheep (Amnotragus lervia) asociated with Chromobacterium violaceum (1996) Veterin. Record., 138, pp. 499-500Cha, C., Gao, P., Chen, Y.C., Shaw, P.D., Farrand, S.K., Production of acyl-homoserine lactone quorum-sensing signals by Gram-negative plant-associated bacteria (1998) Mol. Plant Microbe Interact., 11, pp. 1119-1129Chong, C.Y., Lam, M.S., Case report and review of Chromobacterium sepsis-a Gram negative sepsis mimicking melioidosis (1997) Singapore Med. J., 38, pp. 263-265Cheah, E., MacPherson, K., Quiggin, D., Keese, P., Ollis, D.L., Crystallization and preliminary X-ray analysis of IND, an enzyme with indole oxygenase activity from Chromobacterium violaceum (1998) Acta Crystl. Sec. D. Biol. Crystal., 54, pp. 657-658Chen, D.W., Frey, P.A., Phenylalanine hydroxylase from Chromobacterium violaceum. Uncoupled oxidation of tetrahydropterin and the role of iron in hydroxylation (1998) J. Biol. Chem., 273, pp. 25594-25601Chernin, L.S., Winson, M.K., Thompson, J.M., Haran, S., Bycroft, B.W., Chet, I., Williams, P., Stewart, G.S.A.B., Chitinolytic activity in Chromobacterium violaceum: Substrate analysis and regulation by quorum sensing (1998) J. Bacteriol., 180, pp. 4435-4441Christian, M.C., Pluda, J.M., Ho, P.T.C., Arbuck, S.G., Murgo, A.J., Sausville, E.A., Promising new agents under development by the division of cancer treatment, diagnosis, and centers of the National Cancer Institute (1997) Seminars Oncol., 24, pp. 219-240Cimarusti, C., Floyd, D.M., Fritz, A.W., Parker, W.L., Stusarchyk, W.A., Sykes, R.B., US Patent. US4386034-A, 1983Collins, B., Kollias, G., Battles, A., Moreland, A., Chromobacterium violaceum infection in a stumptailed monkey (Macaca artoices) (1985) Lab. Animal Sci., 35, pp. 530-531Cooper, R., Wells, J.S., Sykes, R.B., Novel potentiators of β-lactam antibiotics. Isolation of SQ28,504 and SQ28,546 from Chromobacterium violaceum (1985) J. Antibiotics, 38, pp. 449-454Cooper, R., Sykes, R.B., Wells, J.S., New compounds, SQ28504 and SQ28546 - enhance antibacterial activity of beta-lactam antibiotics SQ28546, US Patent US4752469-A, 1984De Azevedo, M.B.M., Alderete, J., Lino, A.C.S., Loh, W., Faljoni-Alario, A., Durán, N., Violacein/cyclodextrin inclusion complex by diffusion coefficient and circular dichroism measurements (2000) J. Inclusion Phenom. Mol. Recognit. Chem., 37, pp. 67-74De Azevedo, M.B.M., Alderete, J., Rodriguez, J.A., De Souza, A.O., Faljoni-Alario, A., Durán, N., Biological activities of new antitumoral of indole derivatives in a inclusion complex with cyclodextrin (2000) J. Inclusion Phenom. Mol. Recognit. Chem., 37, pp. 93-101De Azevedo, M.B.M., Justo, G.Z., Rettori, D., Rodriguez, J.A., Haun, M., Dur

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Microbiol., 39, pp. 191-195Barocchi, M.A., Ko, A.I., Reis, M.G., McDonald, K.L., Riley, L.W., Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen (2002) Infect. Immun., 70, pp. 6926-6932Boursaux-Eude, C., Saint Girons, I., Zuerner, R., IS1500, an IS3-like element from Leptospira interrogans (1995) Microbiology, 141, pp. 2165-2173Charon, N.W., Goldstein, S.F., Genetics of motility and chemotaxis of a fascinating group of bacteria: The spirochetes (2002) Annu. Rev. Genet., 36, pp. 47-73Chen, C.W., Huang, C.H., Lee, H.H., Tsai, H.H., Kirby, R., Once the circle has been broken: Dynamics and evolution of Streptomyces chromosomes (2002) Trends Genet., 18, pp. 522-529Colombatti, A., Bonaldo, P., Doliana, R., Type A modules: Interacting domains found in several non-fibrillar collagens and in other extracellular matrix proteins (1993) Matrix, 13, pp. 297-306De La Pena-Moctezuma, A., Bulach, D.M., Kalambaheti, T., Adler, B., Comparative analysis of the LPS biosynthetic loci of the genetic subtypes of serovar Hardjo: Leptospira interrogans subtype Hardjoprajitno and Leptospira borgpetersenii subtype Hardjobovis (1999) FEMS Microbiol. Lett., 177, pp. 319-326Delcher, A.L., Kasif, S., Fleischmann, R.D., Peterson, J., White, O., Salzberg, S.L., Alignment of whole genomes (1999) Nucleic Acids Res., 27, pp. 2369-2376Dimroth, P., Wang, H., Grabe, M., Oster, G., Energy transduction in the sodium F-ATPase of Propionigenium modestum (1999) Proc. Natl. Acad. Sci. USA, 96, pp. 4924-4929Dolata, M.M., Van Beeumen, J.J., Ambler, R.P., Meyer, T.E., Cusanovich, M.A., Nucleotide sequence of the heme subunit of flavocytochrome c from the purple phototrophic bacterium, Chromatium vinosum. A 2.6-kilobase pair DNA fragment contains two multiheme cytochromes, a flavoprotein, and a homolog of human ankyrin (1993) J. Biol. Chem., 268, pp. 14426-14431Dunne Jr., W.M., Bacterial adhesion: Seen any good biofilms lately? (2002) Clin. Microbiol. Rev., 15, pp. 155-166Durbin, R., Eddy, S.R., Krogh, A., Mitchison, G., (1998) Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids, , Cambridge University Press, Cambridge, United KingdomEisen, J.A., Heidelberg, J.F., White, O., Salzberg, S.L., Evidence for symmetric chromosomal inversions around the replication origin in bacteria (2000) Genome Biol., 1. , http://genomebiology.com/2000/1/6/RESEARCH/0011, 4 December, posting date. [Online.]Faine, S., Adler, B., Bolin, C., Perolat, P., (1999) Leptospira and Leptospirosis, , MediSci, Melbourne, AustraliaFarr, R.W., Leptospirosis (1995) Clin. Infect. Dis., 21, pp. 1-6Franklin, M.J., Obman, D.E., Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa (2002) J. Bacteriol., 184, pp. 3000-3007Fraser, C.M., Casjens, S., Huang, W.M., Sutton, G.G., Clayton, R., Lathigra, R., White, O., Venter, J.C., Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi (1997) Nature, 390, pp. 580-586Fraser, C.M., Norris, S.J., Weinstock, G.M., White, O., Sutton, G.G., Dodson, R., Gwinn, M., Venter, J.C., Complete genome sequence of Treponema pallidum, the syphilis spirochete (1998) Science, 281, pp. 375-388Fukunaga, M., Mifuchi, I., Unique organization of Leptospira interrogans rRNA genes (1989) J. Bacteriol., 171, pp. 5763-5767Fulop, V., Ridout, C.J., Greenwood, C., Hajdu, J., Crystal structure of the di-haem cytochrome c peroxidase from Pseudomonas aeruginosa (1995) Structure, 3, pp. 1225-1233Galperin, M.Y., Nikolskaya, A.N., Koonin, E.V., Novel domains of the prokaryotic two-component signal transduction systems (2001) FEMS Microbiol. Lett., 203, pp. 11-21Gilges, D., Vinit, M.A., Callebaut, I., Coulombel, L., Cacheux, V., Romeo, P.H., Vigon, I., Polydom: A secreted protein with pentraxin, complement control protein, epidermal growth factor and von Willebrand factor A domains (2000) Biochem. J., 352, pp. 49-59Godfroid, F., Taminiau, B., Danese, I., Denoel, P., Tibor, A., Weynants, V., Cloeckaert, A., Letesson, J.J., Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophages (1998) Infect. Immun., 66, pp. 5485-5493Haake, D.A., Spirochaetal lipoproteins and pathogenesis (2000) Microbiology, 146, pp. 1491-1504Haake, D.A., Champion, C.I., Martinich, C., Shang, E.S., Blanco, D.R., Miller, J.N., Lovett, M.A., Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic Leptospira spp (1993) J. Bacteriol., 175, pp. 4225-4234Henneberry, R.C., Cox, C.D., Beta-oxidation of fatty acids by Leptospira (1970) Can. J. Microbiol., 16, pp. 41-45Howell, M.L., Alsabbagh, E., Ma, J.F., Ochsner, U.A., Klotz, M.G., Beveridge, T.J., Blumenthal, K.M., Hassett, D.J., AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide (2000) J. Bacteriol., 182, pp. 4545-4556Hunnicutt, D.W., Kempf, M.J., McBride, M.J., Mutations in Flavobacterium johnsoniae gldF and gldG disrupt gliding motility and interfere with membrane localization of GldA (2002) J. Bacteriol., 184, pp. 2370-2378Jovanovic, T., Ascenso, C., Hazlett, K.R., Sikkink, R., Krebs, C., Litwiller, R., Benson, L.M., Rusnak, F., Neelaredoxin, an iron-binding protein from the syphilis spirochete, Treponema pallidum, is a superoxide reductase (2000) J. Biol. Chem., 275, pp. 28439-28448Ko, A.I., Galvao Reis, M., Ribeiro Dourado, C.M., Johnson Jr., W.D., Riley, L.W., Urban epidemic of severe leptospirosis in Brazil (1999) Lancet, 354, pp. 820-825. , Salvador Leptospirosis Study GroupLamothe, G.T., Jolly, L., Mollet, B., Stingele, F., Genetic and biochemical characterization of exopolysaccharide biosynthesis by Lactobacillus delbrueckii subsp. bulgaricus (2002) Arch. Microbiol., 178, pp. 218-228Levett, P.N., Leptospirosis (2001) Clin. Microbiol. Rev., 14, pp. 296-326Lombard, M., Touati, D., Fontecave, M., Niviere, V., Superoxide reductase as a unique defense system against superoxide stress in the microaerophile Treponema pallidum (2000) J. Biol. Chem., 275, pp. 27021-27026Matsunaga, J., Barocchi, M.A., Croda, J., Young, T.A., Sanchez, Y., Siqueira, I., Bolin, C.A., Ko, A.I., Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily (2003) Mol. Microbiol., 49, pp. 929-945Meidanis, J., Braga, M.D., Verjovski-Almeida, S., Whole-genome analysis of transporters in the plant pathogen Xylella fastidiosa (2002) Microbiol. Mol. Biol. Rev., 66, pp. 272-299Missiakas, D., Raina, S., The extracytoplasmic function sigma factors: Role and regulation (1998) Mol. Microbiol., 28, pp. 1059-1066Palaniappan, R.U., Chang, Y.F., Jusuf, S.S., Artiushin, S., Timoney, J.F., McDonough, S.P., Barr, S.C., Mohammed, H.O., Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans (2002) Infect. Immun., 70, pp. 5924-5930Paton, A.W., Paton, J.C., Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113 (1999) Infect. Immun., 67, pp. 5930-5937Paulsen, I.T., Nguyen, L., Sliwinski, M.K., Rabus, R., Saier Jr., M.H., Microbial genome analyses: Comparative transport capabilities in eighteen prokaryotes (2000) J. Mol. Biol., 301, pp. 75-100Pizza, M., Scarlato, V., Masignani, V., Giuliani, M.M., Arico, B., Comanducci, M., Jennings, G.T., Rappuoli, R., Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing (2000) Science, 287, pp. 1816-1820Rappuoli, R., Reverse vaccinology, a genome-based approach to vaccine development (2001) Vaccine, 19, pp. 2688-2691Ren, S.X., Fu, G., Jiang, X.G., Zeng, R., Miao, Y.G., Xu, H., Zhang, Y.X., Zhao, G.P., Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing (2003) Nature, 422, pp. 888-893Santos, J.M., Lobo, M., Matos, A.P., De Pedro, M.A., Arraiano, C.M., The gene bola regulates daca (PBP5), dacC (PBP6) and ampC (AmpC), promoting normal morphology in Escherichia coli (2002) Mol. Microbiol., 45, pp. 1729-1740Springer, T.A., Folding of the N-terminal, ligand-binding region of integrin alpha-subunits into a beta-propeller domain (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 65-72Tang, Y.P., Dallas, M.M., Malamy, M.H., Characterization of the Batl (Bacteroides aerotolerance) operon in Bacteroides fragilis: Isolation of a B. fragilis mutant with reduced aerotolerance and impaired growth in in vivo model systems (1999) Mol. Microbiol., 32, p. 139149Van Sluys, M.A., De Oliveira, M.C., Monteiro-Vitorello, C.B., Miyaki, C.Y., Furlan, L.R., Camargo, L.E., Da Silva, A.C., Kitajima, J.P., Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa (2003) J. Bacteriol, 185, pp. 1018-1026Vujaskovic, Z., Batinic-Haberle, I., Rabbani, Z.N., Feng, Q.F., Kang, S.K., Spasojevic, I., Samulski, T.V., Anscher, M.S., A small molecular weight catalytic metalloporphyrin antioxidant with superoxide dismutase (SOD) mimetic properties protects lungs from radiation-induced injury (2002) Free Radic. Biol. Med., 33, pp. 857-863Werts, C., Tapping, R.I., Mathison, J.C., Chuang, T.H., Kravchenko, V., Saint Girons, I., Haake, D.A., Ulevitch, R.J., Leptospiral lipopolysaccharide activates cells through a TLR2-dependent mechanism (2001) Nat. Immunol., 2, pp. 346-352Whitfield, C., Roberts, I.S., Structure, assembly and regulation of expression of capsules in Escherichia coli (1999) Mol. Microbiol., 31, pp. 1307-1319Wizemann, T.M., Heinrichs, J.H., Adamou, J.E., Erwin, A.L., Kunsch, C., Choi, G.H., Barash, S.C., Koenig, S., Use of a whole genome approach to identify vaccine molecules affording protection against Streptococcus pneumoniae infection (2001) Infect. Immun., 69, pp. 1593-1598Zuerner, R.L., Nucleotide sequence analysis of IS1533 from Leptospira borgpetersenii: Identification and expression of two IS-encoded proteins (1994) Plasmid, 31, pp. 1-11Zuerner, R.L., Huang, W.M., Analysis of a Leptospira interrogans locus containing DNA replication genes and a new IS, IS1502 (2002) FEMS Microbiol. Lett., 215, pp. 175-18