RNA self-cleavage activated by ultraviolet light-induced oxidation

A novel UV-C-light-induced ribozyme activity was discovered within the highly structured 5′-genomic regions of both Hepatitis C Virus (HCV) and the related Classic Swine Fever Virus (CSFV). Cleavage is mediated by exposure to UV-C light but not by exogenous oxygen radicals. It is also very selective, occurring at base positions HCV C 79 and CSFV A45 in some molecules and at the immediately adjacent 5′-positions HCV U 78 and CSFV U 44 in others. Among other reaction products, the majority of biochemically active products detected contained 3′-phosphate and 5′-phosphate-end groups at the newly generated termini, along with a much lower amount of 3′-hydroxyl end group. While preservation of an E-loop RNA structure in the vicinity of the cleavage site was a requisite for HCV RNA self-cleavage, this was not the case for CSFV RNA. The short size of the reactive domains (∼33nt), which are compatible with primitive RNA motifs, and the lack of sequence homology, indicate that as-yet unidentified UV-activated ribozymes are likely to be found throughout structured RNAs, thereby providing clues to whether early RNA self-cleavage events were mediated by photosensitive RNA structures. © 2012 The Author(s).http://dx.doi.org/10.1093/nar/gkr82

Association Between Insulin‐Like Growth Factor‐1 and Social Cognition in Huntington's Disease

Insulin-like growth factor 1 (IGF-1) seems to be involved in the neural circuits associated with social cognition and brain structure.Study funded by Gerencia Regional de Salud de Castilla y Leon (Sacyl GRS 1768/A/18)

Rescate de un ARN sintético en partículas infectivas obtenidas a partir de plásmidos recombinantes que codifican las 10 proteínas del virus de la gripe

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 10-05-199

Uso de la RNasa P como agente antiviral

Filing Date: (2009-11-11).-- Priority Data: ES P200803224 (2008-11-11).-- Titulares: Consejo Superior de Investigaciones Científicas (CSIC), Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto Nacional de Investigaciones Agrarias (INIA), Universidad de Castilla La Mancha (UCLM).[EN] Use of the RNase P of Synechocysitis sp. to inhibit replication of RNA virus and for the production of medicaments for the treatment of diseases caused by RNA virus.[ES] Uso de la RNasa P de Synechocysitis sp. para inhibir la replicación de virus de RNA, y para la elaboración de medicamentos para el tratamiento de enfermedades provocadas por virus de RNA.Peer reviewedConsejo Superior de Investigaciones Científicas (España), Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas, Instituto Nacional de Investigaciones Agrarias (INIA), Universidad de Castilla la ManchaA2 Solicitud de patentes sin informe sobre el estado de la técnic