Effects of lipoproteins on cyclo-oxygenase and lipoxygenase pathways in human platelets

The products of arachidonic acid (AA) metabolism in platelets play an important role in platelet shape change, adhesion and aggregation which may participate in the pathogenesis of ischemic heart disease and thrombosis. Since lipoproteins are also involved in the pathogenesis of thrombo-embolic disorders, the effect of human lipoproteins (HDL, LDL, VLDL) on AA metabolism in human platelets was investigated. Lipoproteins were separated by density gradient zonal ultracentrifugation. The effects of lipoproteins on production of AA metabolites in human platelets i.e., thromboxane A2 (TXA2) and hydroxy-eicosatetraenoic acids (HETEs) were examined using radiometric thin layer chromatography coupled with automated data integrator system. In human platelets, HDL inhibited 12-HETE and TXA2 formation in a concentration-dependent manner. LDL had a strong inhibitory effect on TXA2 production and a weak inhibitory effect on 12-HETE production. VLDL had no effect on platelet AA metabolism. These findings point to a new facet of lipoproteins action and suggest that lipoproteins may have a physiological role in the regulation of AA metabolism in platelets

Impact of Long-Term Loading on Reinforced Concrete Beams Made with Partial Replacement of Coarse Aggregates with Recycled Aggregates from Old Concrete

This research work presents an experimental evaluation of the effect of sustained loading on reinforced concrete beams made with 50% replacement of natural coarse aggregates with aggregates from old concrete. A total of 9 RC beams were cast, 6 with natural coarse aggregates and 3 with recycled concrete aggregates. All beams were cured for 28 days in standard fashion. Among the first batch, 3 beams were used to test under short term loading till failure. An application of 50% of short-term loading followed on all beams assembled on purpose made frames. All beams were monitored for deflection, strain, and cracking. Both deflection and strain remained within limits. Crack monitoring revealed maximum crack width 0.5 mm. After six months of sustained load, beams were removed from the frames and tested in universal load testing machine with central point load till failure. The load-deflection pattern of all beams was observed to be almost similar and 22.12% higher in recycled concrete beams than all-natural aggregate beams. The results show promising use of recycled concrete aggregates in new concrete subjected to sustained loading

Flexural Stress-Strain Behavior of RC Beams Made with Partial Replacement of Coarse Aggregates with Coarse Aggregates from Old Concrete: Part-1: 1:2:4 Ratio

Occupancy, particularly in urban areas, requires more space than ever. Space constraints need erection of high rise buildings in place of short height buildings. This need demolishing of old structures which creates huge quantities of demolished concrete. One of its best disposals is its use in new concrete. Therefore, this research work uses 50% replacement of natural coarse aggregates with coarse aggregates from old concrete to study the flexural stress-strain behavior of reinforced concrete beams. Total of 12 reinforced concrete beams (900x150x150 mm) were cast with 2#4 bars in tension and 2#4 bars in compression zones. Ordinary Portland cement with hill sand and crush aggregate was used in 1:2:4 proportions. Water cement ratio used is 0.54. The beams were cast in two batches, one with 100% natural aggregates and another with 50% natural coarse aggregates replaced with coarse aggregates from old concrete. In each batch 3 beams were cured for 7 and 28 days respectively. After curing all beams were tested with central point load. The beams were monitored at regular intervals for load, displacement, strain and load until first crack. The beams under study were compared with the controlled specimen. The results were in good agreement with the normal concrete specimen. Maximum reduction in flexural stress is recorded as 8.8% for 7-day cured beams and 5.52% for 28-day cured specimen. Thus, the use of coarse aggregates from demolished concrete in new concrete is proved to be promising partial replacement of coarse aggregate in terms of flexural stress-strain relationship

Assessment of Wind Power Potential Based on Raleigh Distribution Model: An Experimental Investigation for Coastal Zone

When compared with other renewable energy resources (RER), the wind energy share in the global energy production is increasing rapidly. Currently, the Government of Pakistan (GoP) is moving towards RER, specifically wind and solar energy. In this paper, the wind energy potential of Tando Ghulam Ali, Sindh, Pakistan is explored. For this purpose, one-year wind speed data is considered at various heights through various probability distribution functions (PDFs). Statistical comparison of Rayleigh, gamma, generalized extreme value (GEV) and lognormal PDFs have been done with two methods, namely root mean square error and (R^2) in order to select the best PDF. Results showed that the Rayleigh distribution function is the best at the above mentioned area for finding various factors like site selection and wind power cost per kWh

Spectrum of electroencephalogram finding in Children with newly diagnosed epilepsy –an Experience at a tertiary care hospital.

Epilepsy is the most common neurological disorders that affect all age groups. It is characterized by at least two unprovoked seizures more than 24 hours apart. The most common investigation used for both diagnosis and management is an Electroencephalogram (EEG), which is relatively cheap and easy to perform. However, EEG requires technical expertise and is prone to misinterpretation. Objective: To determine the frequency of abnormal EEG findings in children with newly diagnosed epilepsy at tertiary care hospital

Effect of Water-Cement Ratio on Flexural Strength of RC Beams Made with Partial Replacement of Coarse Aggregates with Coarse Aggregates from Old Concrete

This research paper presents an experimental evaluation of the effect of water-cement ratio on the flexural strength of reinforced concrete beams made with 50% replacement of coarse aggregates with recycled concrete aggregates (RCA). 72 reinforced concrete beams were cast using 0.54, 0.6, 0.65 and 0.70 water-cement ratio. In each ratio, 12 beams were cast using RCA and 3 beams were cast using all-natural coarse aggregates (NCA). Beams were cured for 7 and 28 days. After curing, all beams were tested with central point load in a universal load testing machine. From the obtained results, it is observed that the maximum reduction in flexural strength of RCA beams is about 28% when compared to the 0.54 w/c ratio beams of the same group and 31.75% in comparison to NCA beams cast with same w/c ratio. The maximum deflection and average strain in beams remained within limits. The observed cracking pattern shows shear failure of all beams

Flexural Strength of Reinforced Concrete RAC Beams Exposed to 6-hour Fire – Part 2: Rich Mix

In this research work, experimental investigation on flexural strength of reinforced concrete beams exposed to fire for 6-hours at the temperature of 1000˚C is presented. The beams are made with 50% replacement of natural coarse aggregates with recyclable concrete aggregates. A total of 12 reinforced concrete beams using 1:1.5:3 mix (rich mix) and 0.54 water-cement ratio were cast. The beams were prepared in two groups. Group 1 beams were prepared with 50% recyclable aggregates, whereas group 2 beams were cast with all-natural aggregates (control specimens). All beams were exposed to fire at the above-mentioned temperature followed by testing in universal load testing machine under central point load. Comparison of the results reveals that proposed beams show comparable resistance even after exposed to 6-hours fire at 1000°C

Near Green Synthesis of Porous Graphene from Graphite Using an Encapsulated Ferrate(VI) Oxidant

This is the final version. Available on open access from the American Chemical Society via the DOI in this recordGraphene oxide (GO) is a conventional yet vital precursor for the synthesis of porous graphene (PG). Several strong oxidizing agents such as potassium permanganate and perchlorates are typically used for oxidization of graphite. However, they expose toxic reactants/products that harm the environment. Therefore, a greener approach is desperately needed to oxidize and exfoliate graphite. This study reports for the first time on successful oxidation of graphite by ferrate(VI) compounds via an encapsulation approach. By further reducing GO prepared from this near green route with vitamin C, PG anticipated by many highly important and expanding areas such as water treatment could be readily achieved. X-ray diffraction (XRD), Fourier transform infrared (FTIR) and UV–vis spectroscopy, and scanning electronic microscopy (SEM) along with energy-dispersive spectroscopy confirmed the high yield of GO from the oxidation of graphite. Raman spectroscopy, XRD, and TEM confirmed the formation of high-quality few-layered PG from the reduction of as-prepared GO. The above results demonstrated the practicality of using encapsulated ferrate(VI) compounds to realize green oxidation of graphite and resolve the paradox about the oxidation capability of ferrate(VI). To further illustrate its potential for the removal of emerging and crucial contaminants from water, as-prepared PG was further examined against the contaminants of methyl orange (MeO) dye and ibuprofen (IBU). Taken together, the results revealed that more than 90% removal efficiency could be achieved at a high PG dosage against MeO and IBU. This ground-breaking greener approach opens the door to risk-free, extensive graphene environmental applications.Department of Science and Technology, Government of IndiaNatural Environment Research Council (NERC

Monitoring and evaluation of irrigation and drainage facilities for pilot distributaries in Sindh Province, Pakistan. Volume 2 - Bareji Distributary, Mirpurkhas District. Interim report

Irrigation management / Monitoring / Evaluation / Irrigation canals / Distributary canals / Drainage / Maintenance / Land use / Irrigation practices / Basin irrigation / Furrow irrigation / Water distribution / Equity / Flow discharge / Watercourses / Water table / Groundwater / Water quality / Pakistan / Sindh Province / Mirpurkhas District / Bareji Distributary