1,745 research outputs found
Investigation of the influence of hybrid layers on the life time of hot forging dies
The paper deals with the issues related in the process of drop forging with special attention paid to the durability of forging tools. It presents the results of industrial investigation of the influence of hybrid layers on hot forging dies. The effectiveness of hybrid layers type nitrided layer/PVD coating applied for extending the life of forging tools whose working surfaces are exposed to such complex exploitation conditions as, among others, cyclically varying high thermal and mechanical loads, as well as intensive abrasion at raised temperature. The examination has been performed on a set of forging tools made of Unimax steel and intended for forging steel rings of gear box synchronizer in the factory FAS in Swarzedz (Poland)
Дотик до вічності. Про розроблення нового історико/архітектурного плану м. Керчі
У 2009 р. автору статті було доручено очолити історико-містобудівні дослідження одного з найцікавіших стародавніх міст нашої країни Керчі. Метою досліджень було складання нового історико/архітектурного опорного плану з визначенням історичного ареалу і зони охорони найбільш цінних територій в межах сучасного міста
Anomalous Dynamics of Forced Translocation
We consider the passage of long polymers of length N through a hole in a
membrane. If the process is slow, it is in principle possible to focus on the
dynamics of the number of monomers s on one side of the membrane, assuming that
the two segments are in equilibrium. The dynamics of s(t) in such a limit would
be diffusive, with a mean translocation time scaling as N^2 in the absence of a
force, and proportional to N when a force is applied. We demonstrate that the
assumption of equilibrium must break down for sufficiently long polymers (more
easily when forced), and provide lower bounds for the translocation time by
comparison to unimpeded motion of the polymer. These lower bounds exceed the
time scales calculated on the basis of equilibrium, and point to anomalous
(sub-diffusive) character of translocation dynamics. This is explicitly
verified by numerical simulations of the unforced translocation of a
self-avoiding polymer. Forced translocation times are shown to strongly depend
on the method by which the force is applied. In particular, pulling the polymer
by the end leads to much longer times than when a chemical potential difference
is applied across the membrane. The bounds in these cases grow as N^2 and
N^{1+\nu}, respectively, where \nu is the exponent that relates the scaling of
the radius of gyration to N. Our simulations demonstrate that the actual
translocation times scale in the same manner as the bounds, although influenced
by strong finite size effects which persist even for the longest polymers that
we considered (N=512).Comment: 13 pages, RevTeX4, 16 eps figure
Natural diversity of potato (Solanum tuberosum) invertases
<p>Abstract</p> <p>Background</p> <p>Invertases are ubiquitous enzymes that irreversibly cleave sucrose into fructose and glucose. Plant invertases play important roles in carbohydrate metabolism, plant development, and biotic and abiotic stress responses. In potato (<it>Solanum tuberosum</it>), invertases are involved in 'cold-induced sweetening' of tubers, an adaptive response to cold stress, which negatively affects the quality of potato chips and French fries. Linkage and association studies have identified quantitative trait loci (QTL) for tuber sugar content and chip quality that colocalize with three independent potato invertase loci, which together encode five invertase genes. The role of natural allelic variation of these genes in controlling the variation of tuber sugar content in different genotypes is unknown.</p> <p>Results</p> <p>For functional studies on natural variants of five potato invertase genes we cloned and sequenced 193 full-length cDNAs from six heterozygous individuals (three tetraploid and three diploid). Eleven, thirteen, ten, twelve and nine different cDNA alleles were obtained for the genes <it>Pain-1</it>, <it>InvGE</it>, <it>InvGF</it>, <it>InvCD141 </it>and <it>InvCD111</it>, respectively. Allelic cDNA sequences differed from each other by 4 to 9%, and most were genotype specific. Additional variation was identified by single nucleotide polymorphism (SNP) analysis in an association-mapping population of 219 tetraploid individuals. Haplotype modeling revealed two to three major haplotypes besides a larger number of minor frequency haplotypes. cDNA alleles associated with chip quality, tuber starch content and starch yield were identified.</p> <p>Conclusions</p> <p>Very high natural allelic variation was uncovered in a set of five potato invertase genes. This variability is a consequence of the cultivated potato's reproductive biology. Some of the structural variation found might underlie functional variation that influences important agronomic traits such as tuber sugar content. The associations found between specific invertase alleles and chip quality, tuber starch content and starch yield will facilitate the selection of superior potato genotypes in breeding programs.</p
Dragging a polymer chain into a nanotube and subsequent release
We present a scaling theory and Monte Carlo (MC) simulation results for a
flexible polymer chain slowly dragged by one end into a nanotube. We also
describe the situation when the completely confined chain is released and
gradually leaves the tube. MC simulations were performed for a self-avoiding
lattice model with a biased chain growth algorithm, the pruned-enriched
Rosenbluth method. The nanotube is a long channel opened at one end and its
diameter is much smaller than the size of the polymer coil in solution. We
analyze the following characteristics as functions of the chain end position
inside the tube: the free energy of confinement, the average end-to-end
distance, the average number of imprisoned monomers, and the average stretching
of the confined part of the chain for various values of and for the number
of monomers in the chain, . We show that when the chain end is dragged by a
certain critical distance into the tube, the polymer undergoes a
first-order phase transition whereby the remaining free tail is abruptly sucked
into the tube. This is accompanied by jumps in the average size, the number of
imprisoned segments, and in the average stretching parameter. The critical
distance scales as . The transition takes place when
approximately 3/4 of the chain units are dragged into the tube. The theory
presented is based on constructing the Landau free energy as a function of an
order parameter that provides a complete description of equilibrium and
metastable states. We argue that if the trapped chain is released with all
monomers allowed to fluctuate, the reverse process in which the chain leaves
the confinement occurs smoothly without any jumps. Finally, we apply the theory
to estimate the lifetime of confined DNA in metastable states in nanotubes.Comment: 13pages, 14figure
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