THE DIFFERENT ALCOHOL USE AMONG ASIAN POPULATION IN U.S.: THE ROLE OF RACE IN THE RELATINOSHIP BETWEEN PERSONALITY/SOCIAL LEARNING AND ALCOHOL USE

poster abstractAlthough extensive research on alcohol use among Caucasian population has been conducted, there are only a few studies to explore an underlying mechanism of alcohol use on Asian population in U.S. Because of the risks associated with alcohol, it is important to understand the different alcohol use behaviors among Asian group in terms of personality and social learning. Extensive studies have suggested that drinking motives and alcohol expec-tancy are good proxy predictors of social and cultural learning, and impul-sivity is related to personality traits. However, there is only a few data that examine impulsivity, drinking motives, and alcohol expectancy difference by race. No studies have ever examined the mediating and moderating role of race in the relationship between impulsivity and drinking motives/alcohol expectancy on alcohol use. The goal of this poster is to review the different impulsivity, drinking motives, and alcohol expectancy among Asian popula-tion in order to elucidate the unknown mechanism of alcohol use and devel-op a theoretical model of Asian drinking. Our review of the literature indi-cates that Asians particularly endorsed high impulsive coping strategies compared to Caucasians, engaged in problematic drinking due to conformity drinking motives, and alcohol expectancies mediated the relationship be-tween impulsivity and alcohol use only for Asian group. These findings sug-gest that the role of culture and race has crucial influence on Asian drinking pattern in U.S. and needs to be empirically examined in the future studies

Evidence-Based Interventions that Promote Resident Wellness from the Council of Emergency Residency Directors

Initiatives for addressing resident wellness are a recent requirement of the Accreditation Council for Graduate Medical Education in response to high rates of resident burnout nationally. We review the literature on wellness and burnout in residency education with a focus on assessment, individual-level interventions, and systemic or organizational interventions

Adversity to Advantage: New Vacant Land Uses in Flint

http://deepblue.lib.umich.edu/bitstream/2027.42/110962/1/adversity_to_advantage.pd

UC-238 SP4-DogEm

The DogEm project\u27s overarching objective is to produce a functional cross-platform mobile app that reliably sends a specific contact a series of calls, emails, and texts until the contact responds. To accomplish this goal, we have compiled preliminary research, produced a series of prototypes, and started the development process. Our preliminary research consists of research pertaining to our tech stack, our user base, our app\u27s requirements, possibilities for messaging and calling features, UX/UI research, reading through React Native documentation, and constraints on the messaging and calling features on iOS vs. Android. Each member produced a DogEm app prototype to familiarize ourselves with the technology that will be employed throughout the project: React Native and Expo CLI. Features implemented throughout the prototypes include, React Native Navigation, expo-sms, expo Linking, Firebase, and various other React Native libraries and APIs. Our development process currently consists of a team effort in which members are assigned an important functionality of the app. Members then actively contribute their changes to a GitHub repository holding our final product. GitHub is an important tool for our development efforts because it allows for code review and effective version control. With regard to version control, we are tracking and maintaining changes made to code on our GitHub. It is an integral part of our software development process. In order to facilitate best practices in our source code management, the DogEm team has been storing and managing our code in GitHub and maintaining a source code directory on our website

Whole Methylome Analysis by Ultra-Deep Sequencing Using Two-Base Encoding

Methylation, the addition of methyl groups to cytosine (C), plays an important role in the regulation of gene expression in both normal and dysfunctional cells. During bisulfite conversion and subsequent PCR amplification, unmethylated Cs are converted into thymine (T), while methylated Cs will not be converted. Sequencing of this bisulfite-treated DNA permits the detection of methylation at specific sites. Through the introduction of next-generation sequencing technologies (NGS) simultaneous analysis of methylation motifs in multiple regions provides the opportunity for hypothesis-free study of the entire methylome. Here we present a whole methylome sequencing study that compares two different bisulfite conversion methods (in solution versus in gel), utilizing the high throughput of the SOLiD™ System. Advantages and disadvantages of the two different bisulfite conversion methods for constructing sequencing libraries are discussed. Furthermore, the application of the SOLiD™ bisulfite sequencing to larger and more complex genomes is shown with preliminary in silico created bisulfite converted reads

Observations of loading-unloading process at Saturn distant magnetotail

peer reviewe

A pathologically expanded, clonal lineage of IL-21-producing CD4+ T cells drives inflammatory neuropathy.

Inflammatory neuropathies, which include chronic inflammatory demyelinating polyneuropathy (CIDP) and Guillain Barré syndrome (GBS), result from autoimmune destruction of the PNS and are characterized by progressive weakness and sensory loss. CD4+ T cells play a key role in the autoimmune destruction of the PNS. Yet, key properties of pathogenic CD4+ T cells remain incompletely understood. Here, we used paired single-cell RNA-Seq (scRNA-Seq) and single-cell T cell receptor-sequencing (scTCR-Seq) of peripheral nerves from an inflammatory neuropathy mouse model to identify IL-21-expressing CD4+ T cells that were clonally expanded and multifunctional. These IL-21-expressing CD4+ T cells consisted of 2 transcriptionally distinct expanded cell populations, which expressed genes associated with T follicular helper (Tfh) and T peripheral helper (Tph) cell subsets. Remarkably, TCR clonotypes were shared between these 2 IL-21-expressing cell populations, suggesting a common lineage differentiation pathway. Finally, we demonstrated that IL-21 receptor-KO (IL-21R-KO) mice were protected from neuropathy development and had decreased immune infiltration into peripheral nerves. IL-21 signaling upregulated CXCR6, a chemokine receptor that promotes CD4+ T cell localization in peripheral nerves. Together, these findings point to IL-21 signaling, Tfh/Tph differentiation, and CXCR6-mediated cellular localization as potential therapeutic targets in inflammatory neuropathies

Mammalian E-type Cyclins Control Chromosome Pairing, Telomere Stability and CDK2 Localization in Male Meiosis

Loss of function of cyclin E1 or E2, important regulators of the mitotic cell cycle, yields viable mice, but E2-deficient males display reduced fertility. To elucidate the role of E-type cyclins during spermatogenesis, we characterized their expression patterns and produced additional deletions of Ccne1 and Ccne2 alleles in the germline, revealing unexpected meiotic functions. While Ccne2 mRNA and protein are abundantly expressed in spermatocytes, Ccne1 mRNA is present but its protein is detected only at low levels. However, abundant levels of cyclin E1 protein are detected in spermatocytes deficient in cyclin E2 protein. Additional depletion of E-type cyclins in the germline resulted in increasingly enhanced spermatogenic abnormalities and corresponding decreased fertility and loss of germ cells by apoptosis. Profound meiotic defects were observed in spermatocytes, including abnormal pairing and synapsis of homologous chromosomes, heterologous chromosome associations, unrepaired double-strand DNA breaks, disruptions in telomeric structure and defects in cyclin-dependent-kinase 2 localization. These results highlight a new role for E-type cyclins as important regulators of male meiosis