Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp. on lactational performance, metabolism, and digestibility in primiparous and multiparous cows

The objective of this study was to investigate the effects of an exogenous enzyme preparation from As-pergillus oryzae and Aspergillus niger on lactational performance of dairy cows. Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (+/- SD) 36.3 +/- 8.7 kg/d milk yield and 141 +/- 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme prep-aration containing amylolytic and fibrolytic activities (ENZ). After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ra-tion. Production data were collected daily and averaged by week. Milk samples were collected every other week, and milk composition was averaged by week. Blood, fe-cal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment. Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids. Milk fat content tended to be higher in CON cows. A treatment x parity interaction was found for some of the production variables. Primiparous cows receiv-ing ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows. Intake and total-tract digestibility of nutrients did not differ between treatments. Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but 0-hydroxybutyrate concentration tended to be greater in ENZ cows. Overall, the exog-enous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows. The differential production response between pri-miparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.Peer reviewe

Instability of brane cosmological solutions with flux compactifications

We discuss the stability of the higher-dimensional de Sitter (dS) brane solutions with two-dimensional internal space in the Einstein-Maxwel theory. We show that an instability appears in the scalar-type perturbations with respect to the dS spacetime. We derive a differential relation which has the very similar structure to the ordinary laws of thermodynamics as an extension of the work for the six-dimensional model [20]. In this relation, the area of dS horizon (integrated over the two internal dimensions) exactly behaves as the thermodynamical entropy. The dynamically unstable solutions are in the thermodynamically unstable branch. An unstable dS compactification either evolves toward a stable configuration or two-dimensional internal space is decompactified. These dS brane solutions are equivalent to the accelerating cosmological solutions in the six-dimensional Einstein-Maxwell-dilaton theory via dimensional reduction. Thus, if the seed higher-dimensional solution is unstable, the corresponding six-dimensional solution is also unstable. From the effective four-dimensional point of view, a cosmological evolution from an unstable cosmological solution in higher dimensions may be seen as a process of the transition from the initial cosmological inflation to the current dark energy dominated Universe.Comment: 11 pages, 3 figures, references added, to appear in CQ

Lactational performance and plasma and muscle amino acid concentrations in dairy cows fed diets supplying 2 levels of digestible histidine and metabolizable protein

Publisher Copyright: © 2021 American Dairy Science AssociationThe objective of this experiment was to investigate the effect of dietary levels of digestible histidine (dHis) and MP on lactational performance and plasma and muscle concentrations of free AA in dairy cows. A randomized block design experiment was conducted with 48 Holstein cows, including 20 primiparous, averaging (±SD) 103 ± 22 d in milk and 45 ± 9 kg/d milk yield at the beginning of the experiment. A 2-wk covariate period preceded 12 experimental wk, of which 10 wk were for data and sample collection. Experimental treatments were (1) MP-adequate (MPA) diet with 2.1% dHis of MP (MPA2.1), (2) MPA with 3.0% dHis (MPA3.0), (3) MP-deficient (MPD) diet with 2.1% dHis (MPD2.1), and (4) MPD with 3.0% dHis (MPD3.0). Actual dHis supply was estimated at 64, 97, 57, and 88 g/d, respectively. Diets supplied MP at 110% (MPA) and 96% (MPD) of NRC 2001 dairy model requirements calculated based on DMI and production data during the experiment. Dry matter intake and milk yield data were collected daily, milk samples for composition and blood samples for AA analysis were collected every other week, and muscle biopsies at the end of covariate period, and during wk 12 of the experiment. The overall DMI was not affected by dHis or MP level. Milk yield tended to be increased by 3.0% dHis compared with 2.1% dHis. Milk true protein concentration and yield were not affected by treatments, whereas milk urea nitrogen concentration was lower for MPD versus the MPA diet. Milk fat concentration was lower for MPD versus MPA. There was a MP × dHis interaction for milk fat yield and energy-corrected milk; milk fat was lower for MPD3.0 versus MPD2.1, but similar for cows fed the MPA diet regardless of dHis level whereas energy-corrected milk was greater for MPA3.0 versus MPA2.1 but tended to be lower for MPD3.0 versus MPD2.1. Plasma His concentration was greater for cows fed dHis3.0, and concentration of sum of essential AA was greater, whereas carnosine, 1-Methyl-His and 3-Methyl-His concentrations were lower for cows fed MPA versus MPD diet. Muscle concentration of His was greater for cows fed dHis3.0 treatment. The apparent efficiency of His utilization was increased at lower MP and His levels. Overall, cows fed a corn silage-based diet supplying MP at 110% of NRC (2001) requirements tended to have increased ECM yield and similar milk protein yield to cows fed a diet supplying MP at 96% of requirements. Supplying dHis at 3.0% of MP (or 86 and 96 g/d, for MPD3.0 and MPA3.0, respectively) tended to increase milk yield and increased plasma and muscle concentrations of His but had minor or no effects on other production variables in dairy cows.Peer reviewe

Coronavirus Optimization Algorithm: A Bioinspired Metaheuristic Based on the COVID-19 Propagation Model

This study proposes a novel bioinspired metaheuristic simulating how the coronavirus spreads and infects healthy people. From a primary infected individual (patient zero), the coronavirus rapidly infects new victims, creating large populations of infected people who will either die or spread infection. Relevant terms such as reinfection probability, super-spreading rate, social distancing measures, or traveling rate are introduced into the model to simulate the coronavirus activity as accurately as possible. The infected population initially grows exponentially over time, but taking into consideration social isolation measures, the mortality rate, and number of recoveries, the infected population gradually decreases. The coronavirus optimization algorithm has two major advantages when compared with other similar strategies. First, the input parameters are already set according to the disease statistics, preventing researchers from initializing them with arbitrary values. Second, the approach has the ability to end after several iterations, without setting this value either. Furthermore, a parallel multivirus version is proposed, where several coronavirus strains evolve over time and explore wider search space areas in less iterations. Finally, the metaheuristic has been combined with deep learning models, to find optimal hyperparameters during the training phase. As application case, the problem of electricity load time series forecasting has been addressed, showing quite remarkable performance.Ministerio de Economía y Competitividad TIN2017-88209-C

Thermal one- and two-graviton Green's functions in the temporal gauge

The thermal one- and two-graviton Green's function are computed using a temporal gauge. In order to handle the extra poles which are present in the propagator, we employ an ambiguity-free technique in the imaginary-time formalism. For temperatures T high compared with the external momentum, we obtain the leading T^4 as well as the subleading T^2 and log(T) contributions to the graviton self-energy. The gauge fixing independence of the leading T^4 terms as well as the Ward identity relating the self-energy with the one-point function are explicitly verified. We also verify the 't Hooft identities for the subleading T^2 terms and show that the logarithmic part has the same structure as the residue of the ultraviolet pole of the zero temperature graviton self-energy. We explicitly compute the extra terms generated by the prescription poles and verify that they do not change the behavior of the leading and sub-leading contributions from the hard thermal loop region. We discuss the modification of the solutions of the dispersion relations in the graviton plasma induced by the subleading T^2 contributions.Comment: 17 pages, 5 figures. Revised version to be published in Phys. Rev.

Geodesics, the Equivalence Principle and Singularities in Higher-dimensional General Relativity and Braneworlds

The geodesics of a spacetime seldom coincide with those of an embedded submanifold of codimension one. We investigate this issue for higher-dimensional general relativity-like models, firstly in the simpler case without branes to isolate which features are already present, and then in the more complicated case with branes. The framework in which we consider branes is general enough to include asymmetric braneworlds but not thick branes. We apply our results on geodesics to study both the equivalence principle and cosmological singularities. Among the models we study these considerations favour $Z_2$ symmetric braneworlds with a negative bulk cosmological constant.Comment: 20 pages, 2 figures. Accepted by JCAP. Minor proofreading corrections; several references adde