The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase.

The yeast silent information regulator (Sir)2 protein links cellular metabolism and transcriptional silencing through its nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase activity. We report that mitochondria from mammalian cells contain intrinsic NAD-dependent deacetylase activity. This activity is inhibited by the NAD hydrolysis product nicotinamide, but not by trichostatin A, consistent with a class III deacetylase. We identify this deacetylase as the nuclear-encoded human Sir2 homologue hSIRT3, and show that hSIRT3 is located within the mitochondrial matrix. Mitochondrial import of hSIRT3 is dependent on an NH2-terminal amphipathic alpha-helix rich in basic residues. hSIRT3 is proteolytically processed in the mitochondrial matrix to a 28-kD product. This processing can be reconstituted in vitro with recombinant mitochondrial matrix processing peptidase (MPP) and is inhibited by mutation of arginines 99 and 100. The unprocessed form of hSIRT3 is enzymatically inactive and becomes fully activated in vitro after cleavage by MPP. These observations demonstrate the existence of a latent class III deacetylase that becomes catalytically activated upon import into the human mitochondria

Qualification and Lessons Learned with Space Flight Fiber Optic Components

This presentation covers lessons learned during the design, development, manufacturing and qualification of space flight fiber optic components. Changes at NASA, including short-term projects and decreased budgets have brought about changes to vendors and parts. Most photonics for NASA needs are now commercial off the shelf (COTS) products. The COTS Tecnology Assurance approach for space flight and qualification plans are outlined

Space Flight LiDARs, Navigation & Science Instrument Implementations: Lasers, Optoelectronics, Integrated Photonics, Fiber Optic Subsystems and Components

For the past 25 years, the National Aeronautics and Space Administration (NASA) Goddard Space Flight Center's Photonics Group in the Engineering Directorate has been substantially contributing to the flight design, development, production, testing and integration of many science and navigational instruments. The Moon to Mars initiative will rely heavily upon utilizing commercial technologies for instrumentation with aggressive schedule deadlines. The group has an extensive background in screening, qualifying, development and integration of commercial components for spaceflight applications. By remaining adaptable and employing a rigorous approach to component and instrument development, they have forged and fostered relationships with industry partners. They have been willing to communicate lessons learned in packaging, part construction, materials selection, testing, and other facets of the design and production process critical to implementation for high-reliability systems. As a result, this successful collaboration with industry vendors and component suppliers has enabled a history of mission success from the Moon to Mars (and beyond) while balancing cost, schedule, and risk postures. In cases where no commercial components exist, the group works closely with other teams at Goddard Space Flight Center and other NASA field centers to fabricate and produce flight hardware for science, remote sensing, and navigation applications. Summarized here is the last ten years of instrumentation development lessons learned and data collected from the subsystems down to the optoelectronic component level

Sol-gel processing to form doped sol-gel monoliths inside hollow core optical fiber and sol-gel core fiber devices made thereby

A process of fabricating a fiber device includes providing a hollow core fiber, and forming a sol-gel material inside the hollow core fiber. The hollow core fiber is preferably an optical fiber, and the sol-gel material is doped with a dopant. Devices made in this manner includes a wide variety of sensors

Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1.

Hepatitis C virus (HCV) infection is closely tied to the lipid metabolism of liver cells. Here we identify the triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) as a key host factor for HCV infection. DGAT1 interacts with the viral nucleocapsid core and is required for the trafficking of core to lipid droplets. Inhibition of DGAT1 activity or RNAi-mediated knockdown of DGAT1 severely impairs infectious virion production, implicating DGAT1 as a new target for antiviral therapy

Implementation of Commercial Components in Space Flight Optical Science Instruments

The Photonics group top level processes regarding development and qualification of space flight hardware for optoelectronic components along with our contributions and successes with LIDAR systems, space flight reliability heritage, and our testing capabilities are conveyed with this power point presentation

Implementation and Qualifications Lessons Learned for Space Flight Photonic Components

This slide presentation reviews the process for implementation and qualification of space flight photonic components. It discusses the causes for most common anomalies for the space flight components, design compatibility, a specific failure analysis of optical fiber that occurred in a cable in 1999-2000, and another ExPCA connector anomaly involving pins that broke off. It reviews issues around material selection, quality processes and documentation, and current projects that the Photonics group is involved in. The importance of good documentation is stressed

System to Perform Radio Frequency Interferometry Using Optical Fiber Sensing Signal Processing Techniques

Aspects of the present disclosure involve a system and method for performing radio frequency interferometry using optical fiber sensing. Optical fiber sensing is performed as a reference signal is defined and compared, in the optical domain, to incoming signals to obtain interference fringe patterns that can be used to decode phase shift offsets with respect to the designated reference signal. The phase shift offsets can be determined by first optically modulating the reference and incoming signals using a laser source as the carrier. In the optical domain, the reference and incoming signals are combined using an optical coupler and then converted back to the electrical domain for processing

New uses for old tools : Reviving Holdridge Life Zones in soil carbon persistence research

Growing evidence suggests that climate classification facilitates the identification of zones that either agree or disagree with processes explaining soil organic carbon (SOC) persistence. Already forty years ago, Post et al. (1982) posited that the strict temperature and precipitation-based classification defining the Holdridge Life Zones (HLZ) provides a descriptive tool to guide our understanding of the heterogeneous distribution of global SOC stocks. Here we argue that this classification has the potential for describing SOC persistence by linking top-down and bottom-up approaches from different scales, which allows selection of individual regional relevancies necessary to manage and track the fate of our largest terrestrial carbon (C) reservoir. © 2021 The Authors. Journal of Plant Nutrition and Soil Science published by Wiley-VCH Gmb