XUV Free-Electron Laser Experiments on Weakly Bound Dimers: Construction of a Water-Dimer Jet Source and Resonance-Enhanced ICD in Ne₂

The thesis at hand is dealing with two different experiments on dimers, water (H2O) 2 and Neon dimers Ne2 specifically. The first part focuses on the design and construction of an in-vacuum water cluster source, which operates on the principle of supersonic gas expansion. A subsequent commissioning was done to characterize and optimize the performance of the source with respect to the demanded parameters, especially the water dimer yield. Upcoming experiments on proton transfer dynamics in water dimers will employ the developed cluster source. In the second part, an experiment on the resonance-enhanced Interatomic Coulombic Decay (ICD) is presented. It was carried out at the Free-Electron Laser in Hamburg (FLASH), providing high intensities and photon energies in the extreme ultraviolet regime (XUV). By employing the wavelength tunability of FLASH2, we observed an enhancement of ICD, by scanning the Ne+ (2p) -1 -> Ne+ (2s) -1 resonance at 26:9 eV (46:1nm). The resulting Ne++Ne+ fragments were measured with a Reaction Microscope in coincidence

Laser-Doppler gas-velocity instrument

Three-D instrument using a laser light source measures both turbulence and mean velocity of subsonic and supersonic gas flows. This instrument is based on the measurement of the Doppler frequency shift of light waves scattered by moving particles in the gas stream

Resonators coupled to voltage-biased Josephson junctions: From linear response to strongly driven nonlinear oscillations

Motivated by recent experiments, where a voltage biased Josephson junction is placed in series with a resonator, the classical dynamics of the circuit is studied in various domains of parameter space. This problem can be mapped onto the dissipative motion of a single degree of freedom in a nonlinear time-dependent potential, where in contrast to conventional settings the nonlinearity appears in the driving while the static potential is purely harmonic. For long times the system approaches steady states which are analyzed in the underdamped regime over the full range of driving parameters including the fundamental resonance as well as higher and sub-harmonics. Observables such as the dc-Josephson current and the radiated microwave power give direct information about the underlying dynamics covering phenomena as bifurcations, irregular motion, up- and down conversion. Due to their tunability, present and future set-ups provide versatile platforms to explore the changeover from linear response to strongly nonlinear behavior in driven dissipative systems under well defined conditions.Comment: 12 pages, 11 figure

Deconvolution for an atomic distribution: rates of convergence

Let $X_1,..., X_n$ be i.i.d.\ copies of a random variable $X=Y+Z,$ where $X_i=Y_i+Z_i,$ and $Y_i$ and $Z_i$ are independent and have the same distribution as $Y$ and $Z,$ respectively. Assume that the random variables $Y_i$'s are unobservable and that $Y=AV,$ where $A$ and $V$ are independent, $A$ has a Bernoulli distribution with probability of success equal to $1-p$ and $V$ has a distribution function $F$ with density $f.$ Let the random variable $Z$ have a known distribution with density $k.$ Based on a sample $X_1,...,X_n,$ we consider the problem of nonparametric estimation of the density $f$ and the probability $p.$ Our estimators of $f$ and $p$ are constructed via Fourier inversion and kernel smoothing. We derive their convergence rates over suitable functional classes. By establishing in a number of cases the lower bounds for estimation of $f$ and $p$ we show that our estimators are rate-optimal in these cases.Comment: 27 page

Regulation of cargo transfer between ESCRT-0 and ESCRT-I complexes by flotillin-1 during endosomal sorting of ubiquitinated cargo

Ubiquitin-dependent sorting of membrane proteins in endosomes directs them to lysosomal degradation. In the case of receptors such as the epidermal growth factor receptor (EGFR), lysosomal degradation is important for the regulation of downstream signalling. Ubiquitinated proteins are recognised in endosomes by the endosomal sorting complexes required for transport (ESCRT) complexes, which sequentially interact with the ubiquitinated cargo. Although the role of each ESCRT complex in sorting is well established, it is not clear how the cargo is passed on from one ESCRT to the next. We here show that flotillin-1 is required for EGFR degradation, and that it interacts with the subunits of ESCRT-0 and -I complexes (hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) and Tsg101). Flotillin-1 is required for cargo recognition and sorting by ESCRT-0/Hrs and for its interaction with Tsg101. In addition, flotillin-1 is also required for the sorting of human immunodeficiency virus 1 Gag polyprotein, which mimics ESCRT-0 complex during viral assembly. We propose that flotillin-1 functions in cargo transfer between ESCRT-0 and -I complexes

Laser Doppler velocity instrument

Laser Doppler velocity instrument for measuring turbulence and mean velocity in subsonic and supersonic gas flow

An Engineered Calmodulin-Based Allosteric Switch for Peptide Biosensing

This work describes the development of a new platform for allosteric protein engineering that takes advantage of the ability of calmodulin to change conformation upon binding to peptide and protein ligands. The switch we have developed consists of a fusion protein in which calmodulin is genetically inserted into the sequence of TEM1 β-lactamase. In this approach, calmodulin acts as the input domain, whose ligand-dependent conformational changes control the activity of the β-lactamase output domain. The new allosteric enzyme exhibits up to 120 times higher catalytic activity in the activated (peptide bound) state compared to the inactive (no peptide bound) state in vitro. Activation of the enzyme is ligand-dependent-peptides with higher affinities for wild-type calmodulin exhibit increased switch activity. Calmodulin's ability to "turn on" the activity of β-lactamase makes this a potentially valuable scaffold for the directed evolution of highly specific biosensors for detecting toxins and other clinically relevant biomarkers.Chemistry and Chemical Biolog

Quantum mechanical Carnot engine

A cyclic thermodynamic heat engine runs most efficiently if it is reversible. Carnot constructed such a reversible heat engine by combining adiabatic and isothermal processes for a system containing an ideal gas. Here, we present an example of a cyclic engine based on a single quantum-mechanical particle confined to a potential well. The efficiency of this engine is shown to equal the Carnot efficiency because quantum dynamics is reversible. The quantum heat engine has a cycle consisting of adiabatic and isothermal quantum processes that are close analogues of the corresponding classical processes.Comment: 10 page

Swimming Efficiency of Bacterium Escherichia Coli

We use in vivo measurements of swimming bacteria in an optical trap to determine fundamental properties of bacterial propulsion. In particular, we determine the propulsion matrix, which relates the angular velocity of the flagellum to the torques and forces propelling the bacterium. From the propulsion matrix dynamical properties such as forces, torques, swimming speed and power can be obtained from measurements of the angular velocity of the motor. We find significant heterogeneities among different individuals even though all bacteria started from a single colony. The propulsive efficiency, defined as the ratio of the propulsive power output to the rotary power input provided by the motors, is found to be 0.2%.Comment: 6 page