41 research outputs found
Aid for Trade and the Quality of Exports
The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.This paper studies how aid for trade (AfT) affects the quality of recipient countriesâ exports. It shows that the quality effect is most discernible for AfT for assistance in trade policy: a 50% increase in the value of AfT received in this category is associated with a 0.5%-1% increase in the quality of exports to the donor and other OECD countries. On average, the actual AfT received for assistance in trade policy leads to a 2% upgrade of the recipient country in the quality ladder of all developing countries. Around half of this quality effect is driven by the quality improvement of continued products in continued markets (intensive margin), and the other half by the quality upgrading of new products in continued markets and existing products in new markets (extensive margin)
Engineering Escherichia coli for succinate production from hemicellulose via consolidated bioprocessing
<p>Abstract</p> <p>Background</p> <p>The recalcitrant nature of hemicellulosic materials and the high cost in depolymerization are the primary obstacles preventing the use of xylan as feedstock for fuel and chemical production. Consolidated bioprocessing, incorporating enzyme-generating, biomass-degrading and bioproduct-producing capabilities into a single microorganism, could potentially avoid the cost of the dedicated enzyme generation in the process of xylan utilization. In this study, we engineered <it>Escherichia coli </it>strains capable of exporting three hemicellulases to the broth for the succinate production directly from beechwood xylan.</p> <p>Results</p> <p>Xylanases were extracellular environment-directed by fusing with OsmY. Subsequently, twelve variant OsmY fused endoxylanase-xylosidase combinations were characterized and tested. The combination of XynC-A from <it>Fibrobacter succinogenes </it>S85 and XyloA from <it>Fusarium graminearum </it>which appeared to have optimal enzymatic properties was identified as the best choice for xylan hydrolysis (0.18 ± 0.01 g/l protein in the broth with endoxylanase activity of 12.14 ± 0.34 U/mg protein and xylosidase activity of 92 ± 3 mU/mg protein at 8 h after induction). Further improvements of hemicellulases secretion were investigated by <it>lpp </it>deletion, <it>dsbA </it>overexpression and expression level optimization. With co-expression of α-arabinofuranosidase, the engineered <it>E. coli </it>could hydrolyze beechwood xylan to pentose monosaccharides. The hemicellulolytic capacity was further integrated with a succinate-producing strain to demonstrate the production of succinate directly from xylan without externally supplied hydrolases and any other organic nutrient. The resulting <it>E. coli </it>Z6373 was able to produce 0.37 g/g succinate from xylan anaerobically equivalent to 76% of that from xylan acid hydrolysates.</p> <p>Conclusions</p> <p>This report represents a promising step towards the goal of hemicellulosic chemical production. This engineered <it>E. coli </it>expressing and secreting three hemicellulases demonstrated a considerable succinate production on the released monosaccharides from xylan. The ability to use lower-cost crude feedstock will make biological succinate production more economically attractive.</p
How Do Neighbourhood and Working Environment Affect Green Commuting in China? A Resident Health Perspective
Commuting contributes to high levels of greenhouse gases and air pollution. The recently advocated âgreen commutingâ, i.e. active and public modes of transport, will be conducive to low-carbon and environmentally friendly transport. A baseline goal of urban planning is to promote health; however, few studies have explored the health-related impacts of environments at both ends of the commute on residentsâ commuting mode choices. To fill the gap, this study proposes to consider the impact of the neighbourhood and working environment on green commuting from a health perspective. Using a sample of 15,886 people from 368 communities in China, three generalised multilevel linear regression models were estimated. Physical and psychological health were combined to further analyse health-related environmental attributes on the commuting choices of residents with different health levels. The results indicate that the working environment exerts more substantial effects on âgreen commutingâ than the neighbourhood environment, especially for workplace satisfaction. Moreover, we found that a good working environment and relationships will significantly encourage the sub-healthy group to choose active commuting. These findings are beneficial for policymakers to consider focusing on reconciling neighbourhood and working environments and meeting the commuting requirements of the less healthy group
Opposing associations of Internet Use Disorder symptom domains with structural and functional organization of the striatum: A dimensional neuroimaging approach
Background: Accumulating evidence suggests brain structural and functional alterations in Internet Use Disorder (IUD). However, conclusions are strongly limited due to the retrospective case-control design of the studies, small samples, and the focus on general rather than symptom-specific approaches. Methods: We here employed a dimensional multi-methodical MRI-neuroimaging design in a final sample of n 5 203 subjects to examine associations between levels of IUD and its symptom-dimensions (loss of control/time management, craving/social problems) with brain structure, resting state and taskbased (pain empathy, affective go/no-go) brain function. Results: Although the present sample covered the entire range of IUD, including normal, problematic as well as pathological levels, general IUD symptom load was not associated with brain structural or functional alterations. However, the symptom-dimensions exhibited opposing associations with the intrinsic and structural organization of the brain, such that loss of control/time management exhibited negative associations with intrinsic striatal networks and hippocampal volume, while craving/social problems exhibited a positive association with intrinsic striatal networks and caudate volume. Conclusions: Our findings provided the first evidence for IUD symptom-domain specific associations with progressive alterations in the intrinsic structural and functional organization of the brain, particularly of striatal systems involved in reward, habitual and cognitive control processes
Political Resource Curse under Authoritarianism: Evidence from China *
Abstract By analyzing a panel constructed on the political turnovers of 4,390 county leaders in China during 1999-2008, we find that the revenue windfalls accrued to these officials from land sales since 1998 have both undermined the effectiveness of the promotion system for government officials and fueled corruption. Instead of rewarding efforts made to boost GDP growth, promotion is now also positively correlated with signaling efforts or specifically increased spending on flamboyant (so-called "image") public projects, with those politically connected to their superiors and those who are beyond the prime age for promotion being the primary beneficiaries. Likewise, the same revenue windfalls have also led to corruption, as gauged from the increases in the government workforce and administrative expenditure but not social welfare spending. Our findings highlight how land revenue windfalls can lead to a political resource curse in a highly politically centralized authoritarian regime
The genome sequence of the orchid Phalaenopsis equestris
Orchidaceae, renowned for its spectacular flowers and other reproductive and ecological adaptations, is one of the most diverse plant families. Here we present the genome sequence of the tropical epiphytic orchid Phalaenopsis equestris, a frequently used parent species for orchid breeding. P. equestris is the first plant with crassulacean acid metabolism (CAM) for which the genome has been sequenced. Our assembled genome contains 29,431 predicted protein-coding genes. We find that contigs likely to be underassembled, owing to heterozygosity, are enriched for genes that might be involved in self-incompatibility pathways. We find evidence for an orchid-specific paleopolyploidy event that preceded the radiation of most orchid clades, and our results suggest that gene duplication might have contributed to the evolution of CAM photosynthesis in P. equestris. Finally, we find expanded and diversified families of MADS-box C/D-class, B-class AP3 and AGL6-class genes, which might contribute to the highly specialized morphology of orchid flowers. (Résumé d'auteur
The Effect of Environmental Regulation on Employment in China: Empirical Research Based on Individual-Level Data
This paper argues that the scale effect and substitution effect in the labor demand for environmental regulations should not be ignored in the pursuit of environmental improvements. It is necessary to analyze the influential mechanism of environmental regulations on employment. Based on the pooled cross-section data combined by CHIP (Chinese Household Income Project) data and macro data at the city level, this paper investigates the impacts of environmental regulations on an individual’s employment probability in China. The results show that there exists a U-shaped relationship between environmental regulations and an individual’s employment probability. The employment effect on workers from different regions and industries or with different hukou (Household Registration System) is heterogeneous. Specifically, the regulations are more stringent in the east, more significant in secondary and tertiary industries, and stronger on urban workers’ employment. The findings are robust to alternative measures
Empirical Analysis for Impact of High-Speed Rail Construction on Interregional Dependency
The opening of the high-speed rail (HSR) resulted in significant changes in the transportation network of Korea. The new HSR construction was expected to become a new engine of local economic growth. However, there was a controversy regarding whether the connection between regions intensifies the concentration of socio-economic activities in the metropolis (straw effect) or contributes to the balance of regional development (sprawl effect). More increasing attention had been devoted to studying the “straw effects” caused by the newly built HSR networks on interregional social-economic activities. Despite considerable research on the benefit achieved from HSR construction, little has focused on the negative externalities resulting from it. This paper examined the potential “straw effects” of two new HSR lines through constructing the indicator of interregional dependency that measured one city’s level of dependency on another one. In order to exclude the interference of lurking variables, five metropolitan cities were selected as case studies. The empirical results, based on a panel data model, revealed that the larger the economic scale of the target city, the lower the level of dependency on other cities, and there existed a “straw effect” on HSR development in terms of Seoul
Traffic Status Dynamic Evaluation of an Eight-Lane Highway Based on the Lane Level
Traffic status analysis is the basis for highway management and control. Most of the previous research studies were carried out from the perspective of road segments. The purpose of this paper is to analyze the difference in traffic operation characteristics of an eight-lane highway from the perspective of lanes. Lane saturation, average lane speed, and lane density were selected as traffic state evaluation indicators, and the concept of state space division was introduced to classify the traffic operation status of each lane. Then, a single-system multi-index dynamic comprehensive evaluation method was adopted to reflect the time-varying characteristics and the development trend of the operation status of each lane. The research results can provide the theoretical support for more refined highway management and improve the service level of the eight-lane highway
Assessing the Distribution of Commuting Trips and Jobs-Housing Balance Using Smart Card Data: A Case Study of Nanjing, China
The purpose of this research is to assess the distribution of commuting trips and the level of jobs-housing balance with Nanjing smart card data. A new approach is presented using the Lorenz curve and Gini coefficient based on the commuting time. This article also quantifies and visualizes Nanjing’s jobs-housing balance in each urban, suburban and exurban district. The core findings from this research are summarized as follows. First, the Gini coefficient of commuting time is 0.251 in urban areas, 0.258 for suburban areas and 0.267 for exurban areas. The gap of each non-urban district in commuting time is larger than urban districts. Second, the result of jobs-housing ratio (JHR) shows that jobs of Xuanwu district are far more than the working population of this district, whereas jobs and working population in other urban districts are relatively matched. The value of JHR is less than 0.8 in all suburban and exurban districts but Yuhuatai district, which suggests that jobs in these suburban districts (excluding Yuhuatai district) are in short supply compared with their working population. Third, the JHR within a particular district may be different according to the specific locations, especially those areas close to the boundary between two different kinds of districts