Relations of POD modes and Lyapunov exponents to the nonlinear dynamic states in flow over oscillating tandem cylinders

Nonlinear dynamic states of flow field are analyzed using the Proper Orthogonal Decomposition (POD) to extract spatial coherent structures, and the temporal development of these spatial structures is further investigated by looking into the largestLyapunov exponents (LLEs) of the temporal coefficients of the POD modes. Additionally, the LLE of vorticity history at a specific point in the wake is also calculated to compare with the LLEs of the modes in the wake region. The flow field used for the analysis is flow around a two-tandem cylinder system with the downstream cylinder oscillating transversely. The numerical simulation is based on an immersed-boundary method for flow with the Reynolds number 100. Three states of typical nonlinear responses of this system are analyzed: lockin, transitional, and quasiperiodic. The results reveal that the paired POD modes can appear in the energetic modes, although this pattern fades in the transitional state. All the LLEs of the energetic modes in the lock-in state are zero, which means that the energetic modes in the lock-in state are stable. For the transitional state, after a long period of time, the LLEs of the first two energetic modes are close to zero, while the others are positive, showing that only the first two modes are relatively stable and that weak chaotic motions exist in the other modes. The LLEs of all the energetic modes in the quasiperiodic state are no larger than zero after a long period of time, resulting in asymptotical stableness of the energetic modes

Proper orthogonal decomposition and recurrence map for the identification of spatial–temporal patterns in a low-Re wake downstream of two cylinders

Flow decomposition methods provide systematic ways to extract the flow modes, which can be regarded as the spatial distribution of a coherent structure. They have been successfully used in the study of wake, boundary layer, and mixing. However, real flow structures also possess complex temporal patterns that can hardly be captured using the spatial modes obtained in the decomposition. In order to analyze the temporal variation of coherent structures in a complex flow field, this paper studies the recurrence in phase space to identify the pattern and classify the evolution of the flow modes. The recurrence pattern depends on the time delay and initial condition. In some cases, the flow system will revisit a previous state regardless of the initial state, and in other cases, the system’s recurrence will depend on the initial state. These patterns are determined by the arrangement and interactions of coherent structures in the flow. The temporal order of the repetition pattern reflects the possible ways of flow evolution

Proper Orthogonal Decomposition and Recurrence Map for the Identification of Spatial–Temporal Patterns in a Low-Re Wake Downstream of Two Cylinders

Flow decomposition methods provide systematic ways to extract the flow modes, which can be regarded as the spatial distribution of a coherent structure. They have been successfully used in the study of wake, boundary layer, and mixing. However, real flow structures also possess complex temporal patterns that can hardly be captured using the spatial modes obtained in the decomposition. In order to analyze the temporal variation of coherent structures in a complex flow field, this paper studies the recurrence in phase space to identify the pattern and classify the evolution of the flow modes. The recurrence pattern depends on the time delay and initial condition. In some cases, the flow system will revisit a previous state regardless of the initial state, and in other cases, the system’s recurrence will depend on the initial state. These patterns are determined by the arrangement and interactions of coherent structures in the flow. The temporal order of the repetition pattern reflects the possible ways of flow evolution

In vitro culturing of porcine tracheal mucosa as an ideal model for investigating the influence of drugs on human respiratory mucosa

It has been previously shown that fresh mucosa from different mammals could serve as raw material for in vitro culturing with the differentiation of cilia, which are the most important morphological structures for the function of the mucociliary system. Increasing legal restrictions on the removal of human tissue and changing surgical techniques have led to a lack of fresh human mucosa for culturing. Most of the animals that have been used as donors up to now are genetically not very close to human beings and must all be sacrificed for such studies. We, therefore, established a modified system of culturing mucosa cells from the trachea of pigs, which is available as a regular by-product after slaughtering. With respect to the possibility of developing “beating” cilia, it could be shown that the speed of cell proliferation until adhesion to the coated culture dishes, the formation of conjunctions of cell clusters and the proliferation of cilia were comparable for porcine and human mucosa. Moreover, it could be demonstrated that the porcine cilia beat frequency of 7.57 ± 1.39 Hz was comparable to the human mucosa cells beat frequency of 7.3 ± 1.4 Hz and that this beat frequency was absolutely constant over the investigation time of 360 min. In order to prove whether the reaction to different drugs is comparable between the porcine and human cilia, we initially tested benzalkonium chloride, which is known to be toxic for human cells, followed by naphazoline, which we found in previous studies on human mucosa to be non-toxic. The results clearly showed that the functional and morphological reactions of the porcine ciliated cells to these substances were similar to the reaction we found in the in vitro cultured human mucosa

Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation

The viability of recalcitrant seeds is lost following stress from either drying or freezing. Reactive oxygen species (ROS) resulting from uncontrolled metabolic activity are likely responsible for seed sensitivity to drying. Nitric oxide (NO) and the ascorbate-glutathione cycle can be used for the detoxification of ROS, but their roles in the seed response to desiccation remain poorly understood. Here, we report that desiccation induces rapid accumulation of H2O2, which blocks recalcitrant Antiaris toxicaria seed germination; however, pretreatment with NO increases the activity of antioxidant ascorbate-glutathione pathway enzymes and metabolites, diminishes H2O2 production and assuages the inhibitory effects of desiccation on seed germination. Desiccation increases the protein carbonylation levels and reduces protein S-nitrosylation of these antioxidant enzymes; these effects can be reversed with NO treatment. Antioxidant protein S-nitrosylation levels can be further increased by the application of S-nitrosoglutathione reductase inhibitors, which further enhances NO-induced seed germination rates after desiccation and reduces desiccation-induced H2O2 accumulation. These findings suggest that NO reinforces recalcitrant seed desiccation tolerance by regulating antioxidant enzyme activities to stabilize H2O2 accumulation at an appropriate concentration. During this process, protein carbonylation and S-nitrosylation patterns are used as a specific molecular switch to control antioxidant enzyme activities

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

ストレス下雌ラットにおける卵巣及び副腎機能に対するサトウキビエキス摂取の効果に関する研究

博士(獣医学)岐阜大学(Gifu University

Acute Effects of Vagotomy on Baroreflex Equilibrium Diagram in Rats with Chronic Heart Failure

The arterial baroreflex system can be divided into the neural arc, from pressure input to efferent sympathetic nerve activity (SNA), and the peripheral arc, from SNA to arterial pressure (AP). Plotting the neural and peripheral arcs on a pressure–SNA plane yields a baroreflex equilibrium diagram. We examined the effects of vagotomy on the open-loop static characteristics of the carotid sinus baroreflex in normal control rats (NC, n = 10) and rats with heart failure after myocardial infarction (MI, n = 10). In the NC group, vagotomy shifted the neural arc toward higher SNA and decreased the slope of the peripheral arc. Consequently, the operating-point SNA increased without a significant change in the operating-point AP on the baroreflex equilibrium diagram. These vagotomy-induced effects were not observed in the MI group, suggesting a loss of vagal modulation of the carotid sinus baroreflex function in heart failure