438 research outputs found
The Effects, Perceptions, and Attitudes of Previously Sedentary Individuals Starting an Exercise Program
Background: Many Americans do not eat healthfully or exercise, which is associated with higher risk of disease. Nutrition and exercise awareness have increased, but people experience barriers preventing lifestyle change. Limited research has been completed on the impact of nutrition education on dietary intake.
Objective: Examine whether starting an exercise program with or without nutrition education can influence dietary choices in previously sedentary individuals, and to determine their perceptions and attitudes.
Design: Mixed methods study gathering quantitative data through a food frequency questionnaire, InBody test, and demographics/exercise activity survey. Qualitative data was gathered through focus groups. Participants completed six weeks of exercise.
Participants: Male or female between 20 and 50 years who had been previously sedentary for at least 6 months but had started exercising between one week and three months. They had to be able to perform moderate intensity exercise at least two days per week for thirty minutes. Participants were excluded if they were pregnant (self-reported) or injured to prevent exercise. Six participants completed the study, with four participating in focus groups.
Intervention: Group A received nutrition education. Group B did not.
Main outcomes: Food choices, weight, lean body mass, body fat percentage, visceral fat, and perceptions on diet and exercise.
Statistical Analyses Performed: The Linear Mix Model was used for InBody data. Independent t-test was used for food frequency data. Dedoose and Otter were used for qualitative data.
Results: Group A significantly decreased in weight and reported eating significantly less sweets than Group B during the final assessment. Three themes emerged: Professional help, self-efficacy, and the power of habits.
Conclusions: Individuals will encounter ambivalence regardless of what stage of change they are in. Nutrition counseling can guide clients to proceed with change. Not everyone will consider changing dietary habits after starting to exercise regardless of nutrition education
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Endoplasmic Reticulum Lumenal Indicators in Drosophila Reveal Effects of HSP-Related Mutations on Endoplasmic Reticulum Calcium Dynamics
Genes for endoplasmic reticulum (ER)-shaping proteins are among the most commonly mutated in hereditary spastic paraplegia (HSP). Mutation of these genes in model organisms can lead to disruption of the ER network. To investigate how the physiological roles of the ER might be affected by such disruption, we developed tools to interrogate its Ca2+ signaling function. We generated GAL4-driven Ca2+ sensors targeted to the ER lumen, to record ER Ca2+ fluxes in identified Drosophila neurons. Using GAL4 lines specific for Type Ib or Type Is larval motor neurons, we compared the responses of different lumenal indicators to electrical stimulation, in axons and presynaptic terminals. The most effective sensor, ER-GCaMP6-210, had a Ca2+ affinity close to the expected ER lumenal concentration. Repetitive nerve stimulation generally showed a transient increase of lumenal Ca2+ in both the axon and presynaptic terminals. Mutants lacking neuronal reticulon and REEP proteins, homologs of human HSP proteins, showed a larger ER lumenal evoked response compared to wild type; we propose mechanisms by which this phenotype could lead to neuronal dysfunction or degeneration. Our lines are useful additions to a Drosophila Ca2+ imaging toolkit, to explore the physiological roles of ER, and its pathophysiological roles in HSP and in axon degeneration more broadly
Kaposi sarcoma incidence in females is nearly four-fold higher in the Lower Rio Grande Valley compared to the Texas average
The Lower Rio Grande Valley (LRGV) is located on U.S.-Mexican border with a population that is 90% Hispanic [1]. Comprised of Hidalgo, Cameron, Starr and Willacy counties, this region has the highest poverty rate and one of the highest incidences of Type 2 diabetes in the United States [2], [3], [4]. Previous studies demonstrated a high prevalence of Human Herpes Virus 8 (HHV8) in the LRGV [5], [6], [7]. HHV8 infection has been causally linked to Kaposi Sarcoma (KS) [8]. Here, we retrospectively examine the incidence of KS in the LRGV in a set of HIV-negative Hispanic patients. Strikingly, the incidence of KS was higher in LRGV women compared to the Texas state average (nearly four-fold higher in McAllen-Edinburg-Pharr Metro Statistical Area). This unique profile aligns with the increased HHV8 prevalence in the LRGV, suggesting that HHV8 contributes to a high incidence of HIV-negative KS on the U.S.–Mexican border in Texas
Ecology and Genetic Structure of a Northern Temperate Vibrio cholerae Population Related to Toxigenic Isolates
Although Vibrio cholerae is an important human pathogen, little is known about its populations in regions where the organism is endemic but where cholera disease is rare. A total of 31 independent isolates confirmed as V. cholerae were collected from water, sediment, and oysters in 2008 and 2009 from the Great Bay Estuary (GBE) in New Hampshire, a location where the organism has never been detected. Environmental analyses suggested that abundance correlates most strongly with rainfall events, as determined from data averaged over several days prior to collection. Phenotyping, genotyping, and multilocus sequence analysis (MLSA) revealed a highly diverse endemic population, with clones recurring in both years. Certain isolates were closely related to toxigenic O1 strains, yet no virulence genes were detected. Multiple statistical tests revealed evidence of recombination among strains that contributed to allelic diversity equally as mutation. This relatively isolated population discovered on the northern limit of detection for V. cholerae can serve as a model of natural population dynamics that augments predictive models for disease emergence
Hookah-related Twitter chatter: A content analysis
INTRODUCTION: Hookah smoking is becoming increasingly popular among young adults and is often perceived as less harmful than cigarette use. Prior studies show that it is common for youth and young adults to network about substance use behaviors on social media. Social media messages about hookah could influence its use among young people. We explored normalization or discouragement of hookah smoking, and other common messages about hookah on Twitter. METHODS: From the full stream of tweets posted on Twitter from April 12, 2014, to May 10, 2014 (approximately 14.5 billion tweets), all tweets containing the terms hookah, hooka, shisha, or sheesha were collected (n = 358,523). The hookah tweets from Twitter users (tweeters) with high influence and followers were identified (n = 39,824) and a random sample of 5,000 tweets was taken (13% of tweets with high influence and followers). The sample of tweets was qualitatively coded for normalization (ie, makes hookah smoking seem common and normal or portrays positive experiences with smoking hookah) or discouragement of hookah smoking, and other common themes using crowdsourcing. RESULTS: Approximately 87% of the sample of tweets normalized hookah use, and 7% were against hookah or discouraged its use. Nearly half (46%) of tweets that normalized hookah indicated that the tweeter was smoking hookah or wanted to smoke hookah, and 19% were advertisements/promotions for hookah bars or products. CONCLUSION: Educational campaigns about health harms from hookah use and policy changes regarding smoke-free air laws and tobacco advertising on the Internet may be useful to help offset the influence of pro-hookah messages seen on social media
The Effects of Traditional versus Ability-Based Physical Training on the Health and Fitness of Custody Assistant Recruits
International Journal of Exercise Science 15(3): 1641-1660, 2022. This study investigated traditional training (TT) versus ability-based training (ABT) in custody assistant recruits. Retrospective analysis was conducted on two recruit classes who completed an 8-week academy. The TT group (18 males, 13 females) followed a physical training model where recruits completed the same exercises with the same intensity; the ABT group (17 males, 12 females) had exercises tailored towards their ability. Pre- and post-academy, recruits were assessed in: body mass; body fat percentage (BF%); resting heart rate (RHR); blood pressure (BP); waist circumference (WC); waist-to-hip ratio (WHR); grip strength; push-ups; sit-ups; YMCA step test recovery HR; 201-m run; and 2.4-km run. Independent samples t-tests evaluated between-class pre-test differences, with significance set at p ≤ 0.05. Paired samples t-tests (p ≤ 0.05) detected within-group training changes. Change scores were calculated for each variable; independent samples t-tests (p ≤ 0.05) compared change scores between groups. The TT group had lower BF%, BP, and WC; and were superior in sit-ups and the 2.4-km run before training. After academy, the TT recruits improved WHR, grip strength, recovery HR, 201-m run, and 2.4-km run, but increased diastolic BP. The ABT recruits decreased BF%, RHR, and WC, and improved push-ups, sit-ups, recovery HR, 201-m run, and the 2.4-km run. ABT recruits had greater positive changes in BF%, RHR, diastolic BP, and sit-ups. TT and ABT recruits generally experienced favorable fitness changes; the degree of positive change tended to be greater for ABT recruits for select tests. Coupled with the diastolic BP increase for TT recruits, this may provide evidence for ABT
Relationships between Isometric Strength and the 74.84-kg (165-lb) Body Drag Test in Law Enforcement Recruits
Prioritizing conservation investments for mammal species globally
We need to set priorities for conservation because we cannot do everything, everywhere, at the same time. We determined priority areas for investment in threat abatement actions, in both a cost-effective and spatially and temporally explicit way, for the threatened mammals of the world. Our analysis presents the first fine-resolution prioritization analysis for mammals at a global scale that accounts for the risk of habitat loss, the actions required to abate this risk, the costs of these actions and the likelihood of investment success. We evaluated the likelihood of success of investments using information on the past frequency and duration of legislative effectiveness at a country scale. The establishment of new protected areas was the action receiving the greatest investment, while restoration was never chosen. The resolution of the analysis and the incorporation of likelihood of success made little difference to this result, but affected the spatial location of these investments
We need you: Influence of Hiring Demand and Modified Applicant Testing on the Physical Fitness of Law Enforcement Recruits
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