Nitrosative stress treatment of E. coli targets distinct set of thiol-containing proteins

Reactive nitrogen species (RNS) function as powerful antimicrobials in host defence, but so far little is known about their bacterial targets. In this study, we set out to identify Escherichia coli proteins with RNS-sensitive cysteines. We found that only a very select set of proteins contain cysteines that undergo reversible thiol modifications upon nitric oxide (NO) treatment in vivo . Of the 10 proteins that we identified, six (AtpA, AceF, FabB, GapA, IlvC, TufA) have been shown to harbour functionally important thiol groups and are encoded by genes that are considered essential under our growth conditions. Media supplementation studies suggested that inactivation of AceF and IlvC is, in part, responsible for the observed NO-induced growth inhibition, indicating that RNS-mediated modifications play important physiological roles. Interestingly, the majority of RNS-sensitive E. coli proteins differ from E. coli proteins that harbour H 2 O 2 -sensitive thiol groups, implying that reactive oxygen and nitrogen species affect distinct physiological processes in bacteria. We confirmed this specificity by analysing the activity of one of our target proteins, the small subunit of glutamate synthase. In vivo and in vitro activity studies confirmed that glutamate synthase rapidly inactivates upon NO treatment but is resistant towards other oxidative stressors.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72397/1/j.1365-2958.2007.05964.x.pd

Comparison of advanced gravitational-wave detectors

We compare two advanced designs for gravitational-wave antennas in terms of their ability to detect two possible gravitational wave sources. Spherical, resonant mass antennas and interferometers incorporating resonant sideband extraction (RSE) were modeled using experimentally measurable parameters. The signal-to-noise ratio of each detector for a binary neutron star system and a rapidly rotating stellar core were calculated. For a range of plausible parameters we found that the advanced LIGO interferometer incorporating RSE gave higher signal-to-noise ratios than a spherical detector resonant at the same frequency for both sources. Spheres were found to be sensitive to these sources at distances beyond our galaxy. Interferometers were sensitive to these sources at far enough distances that several events per year would be expected

Lasers and optics: Looking towards third generation gravitational wave detectors

Third generation terrestrial interferometric gravitational wave detectors will likely require significant advances in laser and optical technologies to reduce two of the main limiting noise sources: thermal noise due to mirror coatings and quantum noise arising from a combination of shot noise and radiation pressure noise. Increases in laser power and possible changes of the operational wavelength require new high power laser sources and new electro-optic modulators and Faraday isolators. Squeezed light can be used to further reduce the quantum noise while nano-structured optical components can be used to reduce or eliminate mirror coating thermal noise as well as to implement all-reflective interferometer configurations to avoid thermal effects in mirror substrates. This paper is intended to give an overview on the current state-of-the-art and future trends in these areas of ongoing research and development.NSF/PHY0555453NSF/PHY0757968NSF/PHY0653582DFG/SFB/407DFG/SFB/TR7DFG/EXC/QUES

Paleobiology of titanosaurs: reproduction, development, histology, pneumaticity, locomotion and neuroanatomy from the South American fossil record

Fil: García, Rodolfo A.. Instituto de Investigación en Paleobiología y Geología. Museo Provincial Carlos Ameghino. Cipolletti; ArgentinaFil: Salgado, Leonardo. Instituto de Investigación en Paleobiología y Geología. General Roca. Río Negro; ArgentinaFil: Fernández, Mariela. Inibioma-Centro Regional Universitario Bariloche. Bariloche. Río Negro; ArgentinaFil: Cerda, Ignacio A.. Instituto de Investigación en Paleobiología y Geología. Museo Provincial Carlos Ameghino. Cipolletti; ArgentinaFil: Carabajal, Ariana Paulina. Museo Carmen Funes. Plaza Huincul. Neuquén; ArgentinaFil: Otero, Alejandro. Museo de La Plata. Universidad Nacional de La Plata; ArgentinaFil: Coria, Rodolfo A.. Instituto de Paleobiología y Geología. Universidad Nacional de Río Negro. Neuquén; ArgentinaFil: Fiorelli, Lucas E.. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica. Anillaco. La Rioja; Argentin

Performance of a full-scale processing cascade that separates agricultural digestate and its nutrients for agronomic reuse

The application of animal manure on agricultural land in the Netherlands is bound by legal limits to prevent the leaching of nitrogen (N) and phosphorus (P) to ground and surface waters. The surplus of animal manure is transported abroad at high costs. In this study, a full-scale cascaded membrane filtration system (GENIUS) comprising two decanter centrifuges, microfiltration (MF) reverse osmosis (RO) and an ion exchanger was monitored. The system processed agricultural digestate from anaerobically co-digested animal manure into two solid fractions (SFs), RO concentrate, MF concentrate and purified water. The goal was to separate P and ammoniacal nitrogen (NH4-N) and remove water from the digestate. From the initial digestate, 66% of P was recovered in the first SF, which constituted 15% of the total mass, without the addition of iron or aluminium salts or polymer flocculants. Another 29% of P was recovered in the MF concentrate and used as a liquid organic fertiliser. Of the P in the initial digestate, 98% was removed before RO. For N, 34% ended up in the RO concentrate and this product can be regarded as an alternative for synthetic N fertiliser as it contains N solely in mineral form. Overall, around 18% of the total mass of initial digestate was discharged as purified water and 31% was locally applied in the form of RO concentrate. We found that aqua regia digestion before chemical analysis can decrease the measured S content of processed digestate. Compared to the transport of raw (unprocessed) digestate, the implementation of the GENIUS system led to a 53% reduction in the mass-weighted average transport distance

Phytoextraction: a review on enhanced metal availability and plant accumulation Fitoextração: uma revisão sobre disponibilidade induzida e acumulação de metais em plantas

Phytoextraction has emerged as a novel approach to clean up metal-polluted soils in which plants are used to transfer toxic metals from soils to shoots. This review provides a synthesis of current knowledge on phytoextraction of metals from soils and their accumulation in plants. The objective is to integrate soil-related (root exudates and chemical amendments) and biological advances to suggest research needs and future directions. As far as can be deduced from the literature, it will be some time before phytoextraction may be established as a commercial technology. For chemically-assisted phytoextraction, research has not shown easily biodegradable compounds to overcome the risks associated with the use of EDTA for poorly available metals in soils. On the other hand, significant progress has been made on the physiological and molecular aspects regarding tolerance and phytoaccumulation of metals in plants. A multidisciplinary approach is warranted to make phytoextraction a feasible commercial technology to remediate metal-polluted soils.<br>A fitoextração é uma tecnologia emergente para despoluição de solos contaminados por metais pesados que usa plantas para transferir metais do solo para a parte aérea, a qual pode ser removida da área poluída. Esta revisão apresenta uma síntese do atual conhecimento sobre fitoextração de metais pesados do solo e sua acumulação em plantas. O objetivo é integrar em uma mesma discussão os avanços relacionados à química do solo (exsudação radicular e adição de agentes quelantes para aumentar a absorção) e à biologia (tolerância a metais e melhoramento genético) visando sugerir futuras pesquisas na área. Embora promissor, o atual estado de desenvolvimento da fitoextração ainda não permite estabelecê-la como uma tecnologia comercial. A pesquisa ainda não encontrou agentes quelantes facilmente biodegradáveis que possam substituir o EDTA na solubilização de metais pouco disponíveis em solos. Entretanto, significativos progressos têm sido feitos no entendimento dos mecanismos fisiológicos e moleculares de tolerância e acumulação de metais em plantas. Uma abordagem multidisciplinar dos vários aspectos que envolvem a fitoextração poderá tornar essa tecnologia econômica e ambientalmente viável a médio prazo