Hydroxytyrosol targets extracellular matrix remodeling by endothelial cells and inhibits both ex vivo and in vivo angiogenesis

This is the preprint version of our manuscript, corresponding to the article that has been 
published in final form at FOOD CHEMISTRY with DOI: 10.1016/j.foodchem.2016.10.111The health benefits of olive oil are attributed to their bioactive compounds, such as hydroxytyrosol. Previously, we demonstrated that hydroxytyrosol inhibits angiogenesis in vitro. The present study aimed to: i) get further insight into the effects of hydroxytyrosol on extracellular matrix remodeling; and ii) test whether hydroxytyrosol is able to inhibit angiogenesis ex vivo and in vivo. Hydroxytyrosol induced a shift toward inhibition of proteolysis in endothelial cells, with decreased expression of extracellular matrix remodeling-enzyme coding genes and increased levels of some of their inhibitors. Furthermore, this work demonstrated that hydroxytyrosol, at concentrations within the range of its content in virgin olive oil that can be absorbed from moderate and sustained virgin olive oil consumption, is a strong inhibitor of angiogenesis ex vivo and in vivo. These results suggest the need for translational studies to evaluate the potential use of hydroxytyrosol for angio-prevention and angiogenesis inhibition in clinical setting.This work was supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER). The “CIBER de Enfermedades Raras” is an initiative from the ISCIII (Spain). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript

Star clusters and the structure of the ISM. Tunnels and wakes in giant extragalactic HII regions

Several structures have been discovered embedded in regions of recent or ongoing star formation, which point to the importance of the interaction between fast moving wind-blowing stars and their environment. Using hydrodynamic simulations, we investigate the passage through the interstellar medium of a supersonic stellar wind source, and show how it can naturally lead to the formation of tubes, channels and swamps of globules as interfaces are crossed. The results are in excellent agreement with observation of 30 Doradus.Comment: 12 pages + 5 figures (GIF format) - Accepted for pub. in Astrophys. J. Letter

Capillary rise of a liquid between two vertical plates making a small angle.

The penetration of a wetting liquid in the narrow gap between two vertical plates making a small angle is analyzed in the framework of the lubrication approximation. At the beginning of the process, the liquid rises independently at different distances from the line of intersection of the plates except in a small region around this line where the effect of the gravity is negligible. The maximum height of the liquid initially increases as the cubic root of time and is attained at a point that reaches the line of intersection only after a certain time. At later times, the motion of the liquid is confined to a thin layer around the line of intersection whose height increases as the cubic root of time and whose thickness decreases as the inverse of the cubic root of time. The evolution of the liquid surface is computed numerically and compared with the results of a simple experiment

On a class of minimum contrast estimators for Gegenbauer random fields

The article introduces spatial long-range dependent models based on the fractional difference operators associated with the Gegenbauer polynomials. The results on consistency and asymptotic normality of a class of minimum contrast estimators of long-range dependence parameters of the models are obtained. A methodology to verify assumptions for consistency and asymptotic normality of minimum contrast estimators is developed. Numerical results are presented to confirm the theoretical findings.Comment: 23 pages, 8 figure

Stripe to spot transition in a plant root hair initiation model

A generalised Schnakenberg reaction-diffusion system with source and loss terms and a spatially dependent coefficient of the nonlinear term is studied both numerically and analytically in two spatial dimensions. The system has been proposed as a model of hair initiation in the epidermal cells of plant roots. Specifically the model captures the kinetics of a small G-protein ROP, which can occur in active and inactive forms, and whose activation is believed to be mediated by a gradient of the plant hormone auxin. Here the model is made more realistic with the inclusion of a transverse co-ordinate. Localised stripe-like solutions of active ROP occur for high enough total auxin concentration and lie on a complex bifurcation diagram of single and multi-pulse solutions. Transverse stability computations, confirmed by numerical simulation show that, apart from a boundary stripe, these 1D solutions typically undergo a transverse instability into spots. The spots so formed typically drift and undergo secondary instabilities such as spot replication. A novel 2D numerical continuation analysis is performed that shows the various stable hybrid spot-like states can coexist. The parameter values studied lead to a natural singularly perturbed, so-called semi-strong interaction regime. This scaling enables an analytical explanation of the initial instability, by describing the dispersion relation of a certain non-local eigenvalue problem. The analytical results are found to agree favourably with the numerics. Possible biological implications of the results are discussed.Comment: 28 pages, 44 figure

Extension of Tycho catalog for low-extinction windows in the galactic bulge

We present in this work secondary catalogs up to $m_{Val} \sim 13$ based on the Tycho reference frame (ESA, 1997) for 12 selected low-extinction fields towards the galactic bulge. The observations have been performed with the Askania-Zeiss Meridian Circle equiped with a CCD camera, located at the Abrah\~ao de Moraes Observatory (Valinhos, Brazil) and operated by the Institute of Astronomy and Geophysics, S\~ao Paulo University. The presented catalog, though not complete, has been designed to help in intensive search programmes (e.g. microlensing and variable searches) and therefore the selected standards have a high astrometric and photometric ($V$ band, approximately) quality. The mean precisions obtained were $0.0018^{s}$ in $\alpha$, 0.013'' in $\delta$, 0.030 for the standard deviation in magnitude and 0.0042 for the magnitude when weighted with the error bars in each night (in the mean, 42 stars for the catalog of each window). Tables B.1 to B.12 are also available in eletronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html.Comment: 16 pages, 6 figures, A&A Latex style. Published in A&A