Persistência da sindrome metabólica em crianças e adolescentes com excesso de peso de acordo com dois críterios diagnósticos: Um estudo longitudinal.

Objective: To investigate the persistence of the metabolic syndrome (MS) in children and adolescents are overweight according to the diagnostic criteria of the World Health Organization (WHO) and National Cholesterol Education Panel Adult Treatment Program- III (NCEP / ATP III). Methods: A longitudinal study was conducted at the Center for Childhood Obesity between April/2009 and April/2012, involving 133 children and adolescents are overweight. The evaluation was made at first and after 24 months of follow up, anthropometry being performed, then the nutritional classification and diagnosis of MS which was subsequently divided into three groups: negative, intermittent and and persistent MS. Descriptive and agreement between the two criteria and also between groups analysis was performed using the Kappa index, using SPSS version 17.0 (95% CI). Results: Among those evaluated, the majority were female (60.9%), and adolescent with severe obesity. Among the components of metabolic syndrome, fasting glucose, blood pressure levels and insulin resistance were reduced in the period. There was no significant correlation between the diagnostic criteria, although it has seen significant coincidence in the group with persistent metabolic syndrome (p = 0.002). Conclusion: A significant similarity between the criteria of WHO and NCEP/ATPIII only in the group with persistent SM, demonstrating sensitivity of the tests, but the use of more specific markers for this age group it is necessary to identify the early SM.Objetivo: Verificar a persistência da síndrome metabólica (SM) em crianças e adolescentes com excesso de peso de acordo com os critérios diagnósticos da Organização Mundial de Saúde (OMS) e do National Cholesterol Education Program- Adult Treatment Panel III (NCEP/ATP III). Métodos: Estudo longitudinal, realizado no Centro de Obesidade Infantil de abril/2009 a abril/2012, envolvendo 133 crian- ças e adolescentes com excesso de peso. A avaliação se deu em um primeiro momento e após 24 meses de seguimento, sendo realizada antropometria, seguida da classificação nutricional e diagnóstico da SM que foi, posteriormente, dividida em três grupos: SM negativa, intermitente e persistente. A análise descritiva e de concordância entre os dois critérios e também entre os grupos se deu através do índice de Kappa, no programa SPSS versão 17.0 (IC95%). Resultados: Entre os avaliados, a maioria era do sexo feminino (60,9%), adolescente e cursava com obesidade acentuada. Dentre os componentes da síndrome metabólica, a glicemia de jejum, os níveis pressóricos e a resistência insulínica sofreram redução no período. Não foi verificada concordância significativa entre os critérios diagnósticos, embora tenha se observado coincidência significativa no grupo que apresentou a síndrome metabólica persistente (p=0,002). Conclusão: Verificou-se semelhança significativa entre os critérios da OMS e NCEP/ATPIII apenas no grupo com persistência da SM, demonstrando sensibilidade dos testes, porém faz-se necessário a utilização de marcadores mais específicos a essa faixa etária a fim de identificar precocemente a SM

Specificity and sensitivity comparative study between phage PVP-S1 and monoclonal antibody as receptor in polydiacetylene vesicles for Salmonella colorimetric detection

Polydiacetylene polymer (PDA) has been intensively studied because of its properties as colour change from blue to red and change from non-fluorescent to fluorescent form due to an external stimulus that lead to a reorientation of the PDA within the organized structure. External stimulus could be temperature, pH, solvent influence, bacteria presence, mechanical stresses and others (Oliveira et al., 2012). Pires et al. (2010) support the hypothesis that such phenomena occurred due to conformational changes associated with the functional group rotation around the simple carbon-carbon bond present in PDA chains. When the backbones of PDA conjugated polymer chains are perturbed, the delocalized π-network induces changes in electronic absorption and emission properties (Huo et al., 1999). For a particular colour change, it is possible to incorporate a compound in the polydiacetylene carboxyl groups that will work as a specific receptor for the bacteria detection. This technology can be used for the detection of pathogens and thus is important to avoid food contamination once the standard technology demands long time and people trained. The selection of the receptor used in the PDA is the first critical step to develop a biosensor with improved selectivity, selectivity and stability. For this reason, the aim of this study was to make a comparative study between two recognition molecules: phage PVP-S1 (Santos et al., 2011) and a monoclonal antibody in the PDA sensor for the detection of Salmonella. Antibodies lack specificity, poor separation efficiency and sensitivity. Phages are extremely specific, withstand harsh environments, are economically and easily produced, show high stability during storage and thus present potential for bacterial detection. Overall the selection of the recognition molecule that show the best features is important to develop a simple and rapid sensor for the industry and consumer’s life. The specificity of the sensor was proven by using Staphylococus aures and Escherichia coli as gram-positive and gram-negative controls, respectively

Physical activity: a strategy to improve antibody response to a SARS-CoV-2 vaccine booster dose in patients with autoimmune rheumatic diseases.

Physical activity associates with improved immunogenicity following a 2-dose schedule of CoronaVac (Sinovac's inactivated SARS-CoV-2 vaccine) in patients with autoimmune rheumatic diseases (ARD). This study evaluates whether physical activity impacts vaccine-induced antibody responses to a booster dose in this population. This was a phase-4 trial conducted in Sao Paulo, Brazil. Patients with ARD underwent a 3-dose schedule of CoronaVac. One month after the booster, we assessed seroconversion rates of anti-SARS-CoV-2 S1/S2 IgG, geometric mean titers of anti-S1/S2 IgG, frequency of positive neutralizing antibodies, and neutralizing activity. Physical activity was assessed through questionnaire. Physically active (n = 362) and inactive (n = 278) patients were comparable for most characteristics; however, physically active patients were younger (P<.01) and had a lower frequency of chronic inflammatory arthritis (P<.01). Adjusted models showed that physically active patients had -2 times odds of seroconversion rates (OR: 2.09; 95% confidence interval, 1.22 to 3.61), -22% greater geometric mean titers of anti-S1/S2 IgG (22.09%; 95% confidence interval, 3.91 to 65.60), and -7% greater neutralizing activity (6.76%; 95% confidence interval, 2.80 to 10.72) than inactive patients. Patients with ARD who are physically active have greater odds of experiencing better immunogenicity to a booster dose of CoronaVac. These results support the recommendation of physical activity to improve vaccination responses, particularly for immunocompromised individuals

No associations between physical activity and immunogenicity in SARS-CoV-2 seropositive patients with autoimmune rheumatic diseases prior to and after vaccination.

To investigate the association between physical activity and immunogenicity among SARS-CoV-2 seropositive patients with autoimmune rheumatic diseases prior to and following a 2-dose schedule of CoronaVac (Sinovac inactivated vaccine). This was a prospective cohort study within an open-label, single-arm, phase 4 vaccination trial conducted in Sao Paulo, Brazil. In this substudy, only SARS-CoV-2 seropositive patients were included. Immunogenicity was assessed by seroconversion rates of total anti-SARS-CoV-2 S1/S2 immunoglobulin G (IgG), geometric mean titers of anti-S1/S2 IgG, frequency of positive neutralizing antibodies, and neutralizing activity before and after vaccination. Physical activity was assessed through a questionnaire. Model-based analyses were performed controlling for age (30 kg/m2), and use of prednisone, immunosuppressants, and biologics. A total of 180 seropositive autoimmune rheumatic disease patients were included. There was no association between physical activity and immunogenicity before and after vaccination. This study suggests that the positive association between physical activity and greater antibody responses seen in immunocompromised individuals following vaccination is overridden by previous SARS-CoV-2 infection, and does not extend to natural immunity

[evidences For Subclinic Chronic Autoimmune Thyroid Disease In Girls With Turner Syndrome].

Patients with Turner syndrome (TS) frequently exhibit transient, recurrent and asymptomatic variations of TSH and/or thyroid hormones (TH). This work was carried out to evaluate thyroid function and structure in patients with TS who had had such variations in hormone concentrations. Our sample comprised 24 patients, 17 less than 20-years old. Evaluation included serum levels of TSH, free T4, total T3, TPO and Tg autoantibodies, thyroid ultrasound (US) and scintigraphy with 99mTc-pertechnetate. Thirteen patients had abnormal TSH and/or TH levels; 23 exhibited US features compatible with chronic thyroid disorder, particularly thyromegaly (established according to volume expected for stature) and heterogeneous echogenicity. Uptake was normal in 21 cases and tracer distribution was homogeneous in 22. The finding of abnormal hormone concentrations was independent of age, length of time since the first similar finding, thyroid autoantibodies, number of abnormalities at US and abnormal scintigraphic findings. Patients aged more than 20 years had higher frequency of thyroid antibodies and heterogeneous echogenicity, and thyroid volume was significantly correlated to length of time since detection of the first hormone variation, indicating progressive thyroid disease. These results suggest that subclinical thyroid dysfunction in TS is due to chronic autoimmune thyroid disease.51401-

Ecological quality of Azorean coastal waters.

International Conference on Coastal Conservation and Management in the Atlantic and Mediterranean. Estoril, Portugal, 12-17 de Abril de 2010