Multivariate Analysis of Genetic Variation in Rapeseed ( Brassica Napus L. )

Designing breeding programs for rapeseed (Brassica napus L.) cultivars with developed seed and oil yields needs information about the genetic variability of characters. In this study, 40 rapeseed genotypes were evaluated for genetic variation and relationships between 12 agro-morphological characters. Brassica species represent a broad range of crops. This redirects the high degree of genetic diversity and allied phenotypic plasticity. Significant differences among the clusters were observed. The first two components with Eigen value were greater than unity contributed a total of 42.77% variation to the divergence. The genotypes were grouped into five clusters. Cluster V contained the maximum number of genotypes (11) and cluster III contained the lowest (4). The highest inter-cluster distance was found between cluster II and cluster V and the lowest between cluster II and cluster IV. The highest intra-cluster distance was noticed for cluster I and the lowest for cluster V. Considering diversity pattern and other agronomic performance lines Nap0837, Nap-0733-1, Nap-2066, Nap-9901, Nap-108 and BARI-8 could be considered apposite parents. This information is suitable for hybrid breeding and encouraging breeders to exchange their germplasm as to enlarge the genetic diversity of breeding accessions

Morphological Characterization of Deepwater Rice Genotypes

The experiments was conducted to study the early establishment morphological characters of advanced deep water rice.The parameters such as plant height, number of leaves, and number of effective & non-effective tillers were studied with different DAS as treatment in field condition. The genotype BR224-2B-2-5, BR5915-B-7, Bazzail-65 &Gabura showed more plant height at 30 DAS & 60 DAS. But HBJ.A.IV & Bazail-65 showed more plant height at 90 DAS & 120 DAS. BR224-2B-2-5 & BR5915-B-7 showed more leaves and tillers at early stage (60 DAS). But HBJ.A.IV &Birpala showed better performance for same characters at later stage (90 DAS, 120 DAS & 150 DAS). The advanced genotype BR224-2B-2-5 is able to establish significantly at early stage as it showed vigorous growth against stress condition present at early stage. HBJ.A.IV and Birpala have potential of producing more leaves and tillers throughout the growth stages and HBJ.A.IV and Birpala is the tall type DWR cultivar as it produced more seedling height and plant height than others. The yield of the genotype BR224-2B-2-5 and BR5915-B-7 are 3.07 t ha-1 and 2.71 t ha-1 respectively. Finally, the advanced genotype BR224-2B-2-5 may be a good source to meet the future challenge

Haplotype Diversity at Sub1 Locus and Allelic Distribution Among Rice Varieties of Tide and Flood Prone Areas of South-East Asia

Single nucleotide polymorphisms and restriction digestion-based haplotype variations among 160 flood prone rice varieties were analyzed with enzymes Alu I and Cac8 I to generate polymorphisms at Sub1A and Sub1C loci (conferring submergence tolerance), respectively. Haplotype associated with phenotype was used to study the haplotype variations at Sub1A and Sub1C loci and to determine their functional influence on submergence tolerance and stem elongation. Three patterns at Sub1A locus, Sub1A0 (null allele), Sub1A1 (does not cut) and Sub1A2 (one SNP), and four patterns at Sub1C locus, Sub1C1, Sub1C2, Sub1C3 and Sub1C4, were generated. Both tolerant Sub1A1 and intolerant Sub1A2 had the same length, but the difference was presence of a restriction site in the Sub1A2, but absent at the Sub1A1. Further, two types of polymorphism were detected at the Sub1C, one included major length polymorphisms (165, 170 and 175 bp) and the other was a single restriction site at different position. Eight haplotypes (different combinations of the two loci), A1C1, A1C2, A1C4, A2C2, A2C4, A0C2, A0C3 and A0C4, were detected among 160 varieties. Haplotype A1C1 was comparatively more related to haplotypes A1C2 and A1C4, having the same Sub1A allele, and these haplotypes were found only in Bangladeshi, Sri Lankan and Indian varieties. Most tolerant varieties in A1C1 haplotype showed slow elongation, having tolerant specific Sub1A1 and Sub1C1 alleles. Further, the varieties Madabaru and Kottamali (A2C2) also showed moderate level of tolerance without Sub1A1 allele. These varieties were different with FR13A and also suspected to carry different novel tolerant genes at other loci. These materials could be used for hybridization with Sub1 varieties for pyramiding additional tolerant specific alleles into a single genotype for improving submergence tolerance in rice

An Analysis of QoS in ZigBee Network Based on Deviated Node Priority

ZigBee is an IEEE 802.15.4 standardized communication protocol. It forms a flawless Wireless Sensor Network (WSN) standard for interoperability at all levels of the network, particularly the application level which most closely touches the user. A large number of devices from different vendors can work seamlessly. These devices act as a network and send huge data traffic to the Coordinator. End devices at different zones have different roles in communication with each other. There has been a lack in executing their requests in a synchronized way based on task priority. This lack leads to massive data traffic loss and degrades the Quality of Service (QoS). One of the challenges is to analyze the QoS parameters in ZigBee network that help to detect the overall network performance. The contribution of this paper is twofold; first, a ZigBee Network is implemented based on node priority. It demonstrates a method to generate a new priority of devices with respect to their existing priority and zones’ priority as well. Second, the QoS is analyzed based on the new priority status for tasks preference purposes. The outcome of this paper shows that the QoS of the network is more conspicuous than non-priority based network

Variation in expression of Sub1 gene confers differential response to submergence tolerance in rice (Oryza sativa L.)

A cluster of three ethylene response factor (ERF) like genes at the Sub1 locus has been identified from rice variety FR13A that confers tolerance to submergence for about 14 days. Restriction digestion-based haplotype variations among 11 rice accessions were analyzed to generate polymorphisms at Sub1C and Sub1A loci. Considering different combinations of SNPs (haplotype), four haplotypes: A1C1, A1C2, A2C2 and A0C2 were detected. Further, using semi-quantitative RT-PCR - Sub1A and Sub1C transcripts in different haplotypes were interpreted in respect with stem elongation and survival for understanding mechanism of submergence tolerance. Expression variations in Sub1 genes in relation with duration of submergence, haplotype diversity and an interaction of loci were found. Differential expression of Sub1A and Sub1C affected the stem elongation and survival of different haplotypes differentially. In tolerant varieties (A1C1 haplotype, IR40931), the highest expressed gene was Sub1A; while expression of Sub1C was lower. Over-expression of Sub1A gene in tolerant varieties, suppressed the stem elongation under submergence, which improved the level of submergence tolerance. In susceptible cultivar (Fulkari), the highest-expressed gene was Sub1C, while the Sub1A was not expressed which showed higher susceptibility. In moderately tolerant varieties (Kottamali), both Sub1A and Sub1C gene transcripts were up-regulated by submergence and higher level of both Sub1A and Sub1C genes transcripts. The varieties in A2C2 haplotype showed moderate level of tolerance without the Sub1A1 allele and a distinct novel mechanism favored the expression of both the Sub1A and Sub1C alleles that confer higher tolerance under complete submergence