Evaluation of microbiological quality of dried baim (Mastacembelus armatus) in Bangladesh

A study was performed for five months to assess the microbiological quality of dried Baim (Mastacembelus armatus) in Sylhet region of Bangladesh. A total of 45 dried samples were randomly collected on monthly basis from three different sources of Sylhet region; one from producer (drying yard of Lamagaji) and others from retail market (Bandar Bazar) and control (prepared in laboratory). The mean total plate count (TPC) of dried Baim from producer, retail market and control were observed 6.20 ± 0.72 ×105, 9.64 ± 1.58 ×105 and 1.61 ± 1.06×105 cfu/g, respectively, whereas, average total fungal count (TFC) were estimated 3.77 ± 0.81 ×103, 4.65 ± 1.08 ×103 and 1.78 ± 0.64 ×103 cfu/g, respectively. TPC and TFC of dried Baim of retail market were found significantly (P<0.05) highest and significantly (P<0.05) lowest in control samples than others. Twenty five samples from each source were analyzed to determine pathogenic E. coli and Salmonella sp. Hundred percent samples of dried Baim of producer and retail market were found contaminated by E. coli whereas; the controlled samples were free of E. coli. Likewise, the dried Baim samples of producer and retail market were contaminated 60% and 80% respectively with Salmonella. Dried baim sample from market was 100%, producer sample was 62% and control sample was 32% contaminated with fungi. The most common fungus species in samples were Aspergillus fumigatus, Fuserium proliferatum and Rhizopus stolonifer. The overall microbiological quality of control samples was comparatively better than the commercially produced dried Baim in Sylhet region

Effect of Temperature and Sonication on The Extraction of Gallic Acid from Labisia Pumila (Kacip Fatimah)

The increasing demand of herbal based product has created great opportunities for global marketing. Labisia pumila contains a phenolic compounds and it has been proven to have multiple biological effects, such as high antioxidant properties and anti inflammatory activity. The Gallic acid(3,4,5-Trihydroxybenzoic acid) is phenolic compounds that exist in Labisia Pumila. Therefore, it is vital to identify a best extraction technique to maximize the performance of the process. Recently, ultrasound-assisted extraction (UAE) widely reported for the extraction of medicinal plants and herbs due to its economic and green technology. This study was carried out to determine the effect of temperature and duty cycle on the performance of UAE in the extraction of gallic acid from Labisia Pumila (Kacip Fatimah).The sonication regiments ( power intensity and duty cycle) were varied to find maximum gallic acid concentration. The low power intensity at 8.66 W/cm2 was used and several duty cycle (10 to 40%) using ultrasonic processor Q700 (700 watts, 20kHz) provided by QSonica, Newtown, U.S.A with a replaceable flat tip ultrasonic probe (sonotrode) made of titanium alloy that had a tip diameter of 12.7 mm and 127 mm length. As the result, the best condition that has been determined for maximum gallic acid extraction was 100 0C with sonication with (0.26±0.02)% w/w or 2630.18 mg/kg . Besides that, the sonication was improved the gallic acid extraction for both 80 0C and 100 0C by 1.4 and 1.5 fold increament respectively .By comparing the effect of temperature, the higher tempperature give the better extraction yield for both with and without sonication. Based on the result can be concluded that, sonication was improved the extraction yield without any chemical aid

An efficient synthetic route tol-γ-methyleneglutamine and its amide derivatives, and their selective anticancer activity

In cancer cells, glutaminolysis is the primary source of biosynthetic precursors, fueling the TCA cycle with glutamine-derived α-ketoglutarate. The enhanced production of α-ketoglutarate is critical to cancer cells as it provides carbons for the TCA cycle to produce glutathione, fatty acids, and nucleotides, and contributes nitrogens to produce hexosamines, nucleotides, and many nonessential amino acids. Efforts to inhibit glutamine metabolism in cancer using amino acid analogs have been extensive.l-γ-Methyleneglutamine was shown to be of considerable biochemical importance, playing a major role in nitrogen transport inArachisandAmorphaplants. Herein we report for the first time an efficient synthetic route tol-γ-methyleneglutamine and its amide derivatives. Many of thesel-γ-methyleneglutamic acid amides were shown to be as efficacious as tamoxifen or olaparib at arresting cell growth among MCF-7 (ER+/PR+/HER2−), and SK-BR-3 (ER−/PR−/HER2+) breast cancer cells at 24 or 72 h of treatment. Several of these compounds exerted similar efficacy to olaparib at arresting cell growth among triple-negative MDA-MB-231 breast cancer cells by 72 h of treatment. None of the compounds inhibited cell growth in benign MCF-10A breast cells. Overall,N-phenyl amides andN-benzyl amides, such as3,5,9, and10, arrested the growth of all three (MCF-7, SK-BR-3, and MDA-MB-231) cell lines for 72 h and were devoid of cytotoxicity on MCF-10A control cells;N-benzyl amides with an electron withdrawing group at theparaposition, such as5and6, inhibited the growth of triple-negative MDA-MB-231 cells commensurate to olaparib. These compounds hold promise as novel therapeutics for the treatment of multiple breast cancer subtypes

Modeling and Prediction of Water Quality: Evidence of Benarpota Station of Sundarbans Region

The Sundarbans contain the world's largest coastal mangrove forest, with an area of about 10,000 km2 (3,900 sq mi), of which about 6,000 km2 (2,300 sq mi) are located in Bangladesh and about 4,000 km2 (1,500 sq mi) in India. Salinity will be the major issue in the future which could destroy the natural ecological balance. The major salinity components are electric conductivity (EC) at low tide and high tide with the corresponding chloride in low tide and high tide. This study develops a SARMA (2, 0, 2) (2, 0, 0) [4] model as it provides the minimum value of model selection criterion (AIC, BIC, SIC, RMSE) compare to all other models. It was found that the bestfitted model for EC in a high tide of study area follows a seasonal autoregressive moving average model of order (2, 0, 2)(2, 0, 0) [4] and chloride in high tide follows SARMA (1, 0, 1) (2, 0, 2) [4]. After fitting the time series model and forecasting the fitted model, the study showed that in both electric conductivity (EC) and salinity (Chloride ppm) increasing in the last three decades. EC crosses the poor water quality level which is more than 4 ppt or 2560 ppm. Salinity also crosses the poor water quality level which is more than 5 ppt or 3200 ppm. The findings of this study would be a potential contribution to make a comprehensive management plan for the long-term conservation and protection of the cultural landscape and mangrove wetlands ecosystem in the Sundarbans region

A circular economy approach for phosphorus removal using algae biochar

In this study, a potential circular economy approach for macroalgae (Ulva ohnoi) was investigated considering the crucial stages of biorefinery. Important stages, such as drying of biomass, production of biochar (pyrolysis), and application of biochar for phosphorus removal were studied and reported. It is important to note that drying macroalgae biomass from an average wet basis moisture content of ca. 70–85% to a moisture content suitable for thermal conversion, ca. 10%, is a challenging task. The physicochemical properties of biomass as well as biochar were characterized and were correlated with their capacity to adsorb phosphorus (P). The initial thermal analysis of macroalgae biomass revealed that the major weight loss occurred between 150 and 550 °C. The kinetics of the pyrolysis process indicate the requirement of higher apparent activation energy in between 232 and 836 kJ mol−1. An increase in the pore diameter, surface area, and pore volume in the biochar was noticed when the temperature of the pyrolysis process was increased. The highest P adsorption (78 mg-P/ g biochar) during the batch experiments was noticed with biochar obtained at 700 °C and can be due to the availability of alkali and alkaline earth metals. The kinetic study for P adsorption was described well by a pseudo second-order model. Biochar produced from macroalgae biomass can be considered as environmentally beneficial and low-cost adsorbent for phosphorus recovery. The biochar after adsorption may be used in agriculture as a slow release fertiliser due to significant amount of brushite

Patients After the Fontan Insights From Computational Fluid Dynamics

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