Unusual accelerated rate of deletions and insertions in toxin genes in the venom glands of the pygmy copperhead (Austrelaps labialis) from kangaroo island

<p>Abstract</p> <p>Background</p> <p>Toxin profiling helps in cataloguing the toxin present in the venom as well as in searching for novel toxins. The former helps in understanding potential pharmacological profile of the venom and evolution of toxins, while the latter contributes to understanding of novel mechanisms of toxicity and provide new research tools or prototypes of therapeutic agents.</p> <p>Results</p> <p>The pygmy copperhead (<it>Austrelaps labialis</it>) is one of the less studied species. In this present study, an attempt has been made to describe the toxin profile of <it>A. labialis </it>from Kangaroo Island using the cDNA library of its venom glands. We sequenced 658 clones which represent the common families of toxin genes present in snake venom. They include (a) putative long-chain and short-chain neurotoxins, (b) phospholipase A₂, (c) Kunitz-type protease inhibitor, (d) CRISPs, (e) C-type lectins and (f) Metalloproteases. In addition, we have also identified a novel protein with two Kunitz-type domains in tandem similar to bikunin.</p> <p>Conclusion</p> <p>Interestingly, the cDNA library reveals that most of the toxin families (17 out of 43 toxin genes; ~40%) have truncated transcripts due to insertion or deletion of nucleotides. These truncated products might not be functionally active proteins. However, cellular trancripts from the same venom glands are not affected. This unusual higher rate of deletion and insertion of nucleotide in toxin genes may be responsible for the lower toxicity of <it>A. labialis </it>venom of Kangroo Island and have significant effect on evolution of toxin genes.</p

Evaluating Structural, Chlorophyll-Based and Photochemical Indices to Detect Summer Maize Responses to Continuous Water Stress

his study evaluates the performance of structural, chlorophyll-based, and photochemical indices to detect maize water status and to assess production based on five years of field experiments (2013–2017) during the primary growth stages. We employed three categories of indicators, including water condition and productive and thermal indicators, to quantify the responses of summer maize under continuous water stress from drought to waterlogging conditions. Furthermore, we adopted several spectral indices to assess their sensitivity to three categories of metrics. The results showed the association is the best between the treatment level and Leaf Water Content (LWC). The waterlogging treatment influenced Leaf Water Potential (LWP) in moderate drought stress. Severe drought stress caused the strongest reduction in productivity from both Leaf Area Index (LAI) and chlorophyll content. In terms of sensitivity of various indices, red-edge-position (REP) was sensitive to maize water conditions LWP, LAI and chlorophyll content. Photochemical Reflectance Index (PRI) and Normalized Difference Vegetation Index (NDVI) were the most and second most sensitive indices to productive indicators, respectively. The results also showed that no indices were capable of capturing the information of Crop Water Stress Index (CWSI)

COVID-19 Prevention: Role of Activated Carbon

Recently, Coronavirus Disease 2019 (COVID-19) has brought the whole world into a pandemic condition, where the number of infected cases and deaths is exponentially high. A number of vaccines are available for this novel virus, but these are in the preliminary stage and are also not available to everyone. As the virus is very contagious, protection and prevention are the best way to survive and get rid of this disease. The virus affects the human body by entering through the nose, mouth, and eyes, so face protection with an appropriate mask is highly advisable. Combined masks made with activated carbon (AC) can effectively adsorb the virus because of its high surface area and broad functional groups. Such combined masks can also control coronavirus transmission by capturing harmful gases and smoke as they help in decreasing the spread of the viru

Ethnomedicinal Value of Antidiabetic Plants in Bangladesh: A Comprehensive Review

The use of conventional drugs to treat metabolic disorders and the pathological consequences of diabetes further increases the complications because of the side effects, and is sometimes burdensome due to relatively higher costs and occasionally painful route of administration of these drugs. Therefore, shifting to herbal medicine may be more effective, economical, have fewer side effects and might have minimal toxicity. The present review amasses a list of ethnomedicinal plants of 143 species belonging to 61 families, from distinctive domestic survey literature, reported to have been used to treat diabetes by the ethnic and local people of Bangladesh. Leaves of the medicinal plants were found leading in terms of their use, followed by fruits, whole plants, roots, seeds, bark, stems, flowers, and rhizomes. This review provides starting information leading to the search for and use of indigenous botanical resources to discover bioactive compounds for novel hypoglycemic drug development

Evaluation of osteogenic potentials of avian demineralized bone matrix in the healing of osseous defects in pigeons

Objectives: To evaluate avian allogeneic demineralized bone matrix (DBM) in the healing of long bone defects as a function of geometry and time in a pigeon model. Study design: Experimental. Animals: Adult rock pigeons (n = 60). Methods: Midshaft ulnar osseous defects were grafted with 2 geometric forms of DBM (tubular vs. chipped) and stabilized with a hybrid fixator. Autologous chips of sternal keel were used in a third group as control. Outcomes were evaluated by radiography and histology/histomorphometry at 4, 8, 12, and 24 weeks postoperatively. Results: Despite an early rapid healing response, autografts plateaued (histologic score and new bone area) by 8 weeks with no significant improvement afterwards. Conversely, allogeneic DBM implants demonstrated continuous temporal improvement in bone healing, and tubular DBM finally outpaced autograft implants after week 12 with values for metrics achieving statistical significance by week 24. Chip DBM was inferior to tubular DBM and autograft. Conclusions: Avian DBM is osteogenic, biocompatible, and safe in orthotopic sites with potential usefulness in avian bone grafting. Implant geometry (shape and size) affects such osteogenic potentials

Impact of Air Pollutant on Human Health in Kushtia Sugar Mill, Bangladesh

Abstract. The study dealt with the concentration of air pollutants emitted from Kushtia sugar mills in Jagati region of Bangladesh in order to evaluate their impact on human health. The dispersion of air pollutants from sugar mill&apos;s chimney was obtained through point source Gaussian dispersion model. The air pollutants were monitored during winter season in 2011-2012. A questionnaire survey was randomly carried out in a small scale at the study area. The result showed that the maximum concentration of SO 2 , NO x and PM 10 were 28.837 µg/m 3 , 76.177 µg/m 3 and 380.339 µg/m 3 respectively. The particulate matter (PM 10 ) concentration was found to be very high whereas sulfur dioxide (SO 2 ) and nitrogen oxide (NO x) concentrations were low at the study area. The calculated value of air pollution index (API) was 88.18 which indicate that heavy air pollution can predispose individuals to heart and lung disease in the study area people. This study revealed that the concentration of particulate matter found in Kushtia sugar mill had exceeded the minimum level according to the WHO standards. The high concentration of PM 10 is suggested to affect human health and environmental conditions in the study area

Analysis on Preparation, Application, and Recycling of Activated Carbon to Aid in COVID-19 Protection

Activated carbon (AC) is an extremely porous carbonaceous adsorptive substance which has a rigid carbon matrix with high surface area and broad functional groups. The structure is connected by chemical bonds; arranged irregularly, generating a highly porous arrangement of corners, crevices, claps, and cracks between the carbon layers. Activated carbons are produced high-temperature and chemical activation of waste biomass. The pores in the lattice network of activated carbon permit the removal of impurities from gaseous and liquid medium through adsorption. At present, the COVID-19 disease is the prime concern around the whole world because of its exponential infections and death rate. There is no medicine for this virus, and protection is the only remedy to survive from this contagious disease. Using a face mask is one of the best methods to get rid of COVID-19. The mask combined with activated carbon can be beneficial for adsorbing and disinfecting the virus as it is the versatile adsorbent for the elimination of the organic, inorganic, and pathogenic contaminants

Drought Hazard Evaluation in Boro Paddy Cultivated Areas of Western Bangladesh at Current and Future Climate Change Conditions

Drought hazard is one of the main hindrances for sustaining food security in Bangladesh, and climate change may exacerbate it in the next several decades. This study aims to evaluate drought hazard at current and future climate change conditions in the Boro paddy cultivated areas of western Bangladesh using simulated climate data from the outputs of three global climate models (GCMs) based on the SRES A1B scenario for the period between 2041 and 2070. The threshold level of Standardized Precipitation Evapotranspiration Index (SPEI) was employed to identify drought events and its probability distribution function (PDF) was applied to create the drought hazard index. The study demonstrates that enhancement of potential evapotranspiration (PET) will surpass that of precipitation, resulting in intensified drought events in future. In addition, the PDFs of drought events will move the upper tail in future period compared to the baseline. The results showed that the southwestern region was more severe to the drought hazard than the northwestern region during the period of 1984 to 2013. From the results of three GCMs, in the mid-century period, drought hazard will slightly increase in the northwestern region and flatten with a decrease in the southwestern region. The outcomes will help to allocate agricultural adaptation plans under climate change condition in Bangladesh