656 research outputs found
Characterization of T-bet and eomes in peripheral human immune cells.
The T-box transcription factors T-bet and Eomesodermin (Eomes) have been well defined as key drivers of immune cell development and cytolytic function. While the majority of studies have defined the roles of these factors in the context of murine T-cells, recent results have revealed that T-bet, and possibly Eomes, are expressed in other immune cell subsets. To date, the expression patterns of these factors in subsets of human peripheral blood mononuclear cells beyond T-cells remain relatively uncharacterized. In this study, we used multiparametric flow cytometry to characterize T-bet and Eomes expression in major human blood cell subsets, including total CD4(+) and CD8(+) T-cells, γδ T-cells, invariant NKT cells, natural killer cells, B-cells, and dendritic cells. Our studies identified novel cell subsets that express T-bet and Eomes and raise implications for their possible functions in the context of other human immune cell subsets besides their well-known roles in T-cells.
The corrigendum regards data and text for the final figure of the manuscript, Figure 7: Subsequent analysis of T-bet levels in human lymphocytes comparing different permeabilization procedures (eBioscience FoxP3 transcription factor kit, BD Pharmingen Cytofix/Cytoperm) has revealed variable findings in the level of T-bet expression detected within certain lymphocyte populations. While this does not change our conclusions for the majority of the populations assessed in this study, B cells in particular show differences under these conditions. Specifically, permeabilization via the eBioscience FoxP3 transcription factor staining buffer set indicates that subpopulations of memory B cells express significantly higher levels of T-bet (MFI) compared to plasmablasts, and that plasmablasts express T-bet only at low levels. Subsequent RNA transcript analysis confirms that plasmablasts express T-bet RNA at a level comparable to naïve B cells. Together, in combination with fluorescence-minus-one and isotype control studies, these new findings suggest that subsets memory B cells, not plasmablasts, express the highest levels of T-bet in the B cell compartment and plasmablasts express T-bet at a lower frequency than is reported in Figure 7. Figure 7 Legend should read: (C) Histograms depicting T-bet expression levels in B-cells and NK cells from a representative donor. Histograms represent the following subsets: naïve B-cells (thick black line), memory B-cells (shaded gray), plasmablasts (thin black line), CD56bright NK cells (gray line), and CD56dim NK cells (shaded black). B-cell results section should be titled T-bet is predominantly expressed in mature memory B-cells and should read: While Eomes was undetectable in B-cells (data not shown), we found T-bet in ~10% of B-cells (Figure 7B). This T-bet expression was largely relegated to memory B-cells, with significantly lower amounts observed in transitional/immature B-cells, naïve B-cells, and plasmablasts (Figure 7B). Greater than 15% of memory B-cells expressed T-bet, a significantly higher frequency than that of all other B-cell populations, suggesting that T-bet may play a particularly important role in memory B-cell function. The discussion related to T-bet expression in plasmablasts should be reconsidered as follows: We found that T-bet is not significantly expressed in transitional/immature B-cells, naïve B-cells, and plasmablasts, but is highly expressed in subsets of memory-B cells. Reduced frequencies of T-bet expression in plasmablasts indicate a specific role for T-bet at the memory B-cell stage of development, which may no longer be necessary after further differentiation to the plasmablast stage. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest
Asynchronous timing and Doppler recovery in DSP based DPSK modems for fixed and mobile satellite applications
While conventional analog modems employ some kind of clock wave regenerator circuit for synchronous timing recovery, in sampled modem receivers the timing is recovered asynchronously to the incoming data stream, with no adjustment being made to the input sampling rate. All timing corrections are accomplished by digital operations on the sampled data stream, and timing recovery is asynchronous with the uncontrolled, input A/D system. A good timing error measurement algorithm is a zero crossing tracker proposed by Gardner. Digital, speech rate (2400 - 4800 bps) M-PSK modem receivers employing Gardner's zero crossing tracker were implemented and tested and found to achieve BER performance very close to theoretical values on the AWGN channel. Nyguist pulse shaped modem systems with excess bandwidth factors ranging from 100 to 60 percent were considered. We can show that for any symmetric M-PSK signal set Gardner's NDA algorithm is free of pattern jitter for any carrier phase offset for rectangular pulses and for Nyquist pulses having 100 percent excess bandwidth. Also, the Nyquist pulse shaped system is studied on the mobile satellite channel, where Doppler shifts and multipath fading degrade the pi/4-DQPSK signal. Two simple modifications to Gardner's zero crossing tracker enable it to remain useful in the presence of multipath fading
A Candidate Young Massive Planet in Orbit around the Classical T Tauri Star CI Tau
The ~2 Myr old classical T Tauri star CI Tau shows periodic variability in
its radial velocity (RV) variations measured at infrared (IR) and optical
wavelengths. We find that these observations are consistent with a massive
planet in a ~9-day period orbit. These results are based on 71 IR RV
measurements of this system obtained over 5 years, and on 26 optical RV
measurements obtained over 9 years. CI Tau was also observed photometrically in
the optical on 34 nights over ~one month in 2012. The optical RV data alone are
inadequate to identify an orbital period, likely the result of star spot and
activity induced noise for this relatively small dataset. The infrared RV
measurements reveal significant periodicity at ~9 days. In addition, the full
set of optical and IR RV measurements taken together phase coherently and with
equal amplitudes to the ~9 day period. Periodic radial velocity signals can in
principle be produced by cool spots, hot spots, and reflection of the stellar
spectrum off the inner disk, in addition to resulting from a planetary
companion. We have considered each of these and find the planet hypothesis most
consistent with the data. The radial velocity amplitude yields an Msin(i) of
~8.1 M_Jup; in conjunction with a 1.3 mm continuum emission measurement of the
circumstellar disk inclination from the literature, we find a planet mass of
~11.3 M_Jup, assuming alignment of the planetary orbit with the disk.Comment: 61 pages, 13 figures, accepted for publication in The Astrophysical
Journa
Deuteron photo-disintegration with polarised photons in the energy range 30 - 50 MeV
The reaction d(\vec\gamma,np) has been studied using the tagged and polarised
LADON gamma ray beam at an energy 30 - 50 MeV to investigate the existence of
narrow dibaryonic resonances recently suggested from the experimental
measurements in a different laboratory. The beam was obtained by Compton
back-scattering of laser light on the electrons of the storage ring ADONE.
Photo-neutron yields were measured at five neutron angle \vartheta_n = 22,
55.5, 90, 125 and 157 degrees in the center of mass system.Our results do not
support the existence of such resonances.Comment: 16 pages, Latex, 22 figures, 1 table. Nucl. Phys. A to appea
Practicum 2012 - 2013: LIFT Boston Client Well Being Study
A Boston based non-profit and a team of public policy PhD students engaged in several months of collaborative problem identification and goal setting focused on the effectiveness of the organization’s unique service delivery model. The nonprofit uses volunteer advocates and a goal-oriented process with no eligibility criteria to assist clients in distress. We collected administrative data, administered a survey, and conducted interviews to explore client well-being
Follow-Up Observations of PTFO 8-8695: A 3 MYr Old T-Tauri Star Hosting a Jupiter-mass Planetary Candidate
We present Spitzer 4.5\micron\ light curve observations, Keck NIRSPEC radial
velocity observations, and LCOGT optical light curve observations of
PTFO~8-8695, which may host a Jupiter-sized planet in a very short orbital
period (0.45 days). Previous work by \citet{vaneyken12} and \citet{barnes13}
predicts that the stellar rotation axis and the planetary orbital plane should
precess with a period of days. As a consequence, the observed
transits should change shape and depth, disappear, and reappear with the
precession. Our observations indicate the long-term presence of the transit
events ( years), and that the transits indeed do change depth, disappear
and reappear. The Spitzer observations and the NIRSPEC radial velocity
observations (with contemporaneous LCOGT optical light curve data) are
consistent with the predicted transit times and depths for the $M_\star = 0.34\
M_\odot$ precession model and demonstrate the disappearance of the transits. An
LCOGT optical light curve shows that the transits do reappear approximately 1
year later. The observed transits occur at the times predicted by a
straight-forward propagation of the transit ephemeris. The precession model
correctly predicts the depth and time of the Spitzer transit and the lack of a
transit at the time of the NIRSPEC radial velocity observations. However, the
precession model predicts the return of the transits approximately 1 month
later than observed by LCOGT. Overall, the data are suggestive that the
planetary interpretation of the observed transit events may indeed be correct,
but the precession model and data are currently insufficient to confirm firmly
the planetary status of PTFO~8-8695b.Comment: Accepted for publication in The Astrophysical Journa
Optical-Model Description of Time-Reversal Violation
A time-reversal-violating spin-correlation coefficient in the total cross
section for polarized neutrons incident on a tensor rank-2 polarized target is
calculated by assuming a time-reversal-noninvariant, parity-conserving
``five-fold" interaction in the neutron-nucleus optical potential. Results are
presented for the system for neutron incident energies
covering the range 1--20 MeV. From existing experimental bounds, a strength of
keV is deduced for the real and imaginary parts of the five-fold
term, which implies an upper bound of order on the relative -odd
strength when compared to the central real optical potential.Comment: 11 pages (Revtex
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