Escherichia coli of sequence type 3835 carrying blaNDM-1, blaCTX-M-15, blaCMY-42 and blaSHV-12

New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. blaNDM-1 was the only carbapenemase gene detected. Strain WCHEC13-8 also had a plasmid-borne AmpC gene (blaCMY-42) and two extended-spectrum β-lactamase genes (blaCTX-M-15 and blaSHV-12). blaNDM-1 and blaSHV-12 were carried by a 54-kb IncX3 self-transmissible plasmid, which is identical to plasmid pNDM-HF727 from Enterobacter cloacae. blaCMY-42 was carried by a 64-kb IncI1 plasmid and blaCTX-M-15 was located on a 141-kb plasmid with multiple F replicons (replicon type: F36:A4:B1). blaCMY-42 was in a complicated context and the mobilisation of blaCMY-42 was due to the transposition of IS Ecp1 by misidentifying its right-end boundary. Genetic context of blaNDM-1 in strain WCHEC13-8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug-resistant ST3835 E. coli clinical strain carrying blaNDM-1, blaCTX-M-15, blaCMY-42 and blaSHV-12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of blaNDM among Enterobacteriaceae in China

Increased human pathogenic potential of Escherichia coli from polymicrobial urinary tract infections in comparison to isolates from monomicrobial culture samples

The current diagnostic standard procedure outlined by the Health Protection Agency for urinary tract infections (UTIs) in clinical laboratories does not report bacteria isolated from samples containing three or more different bacterial species. As a result many UTIs go unreported and untreated, particularly in elderly patients, where polymicrobial UTI samples are especially prevalent. This study reports the presence of the major uropathogenic species in mixed culture urine samples from elderly patients, and of resistance to front-line antibiotics, with potentially increased levels of resistance to ciprofloxacin and trimethoprim. Most importantly, the study highlights that Escherichia coli present in polymicrobial UTI samples are statistically more invasive (P<0.001) in in vitro epithelial cell infection assays than those isolated from monomicrobial culture samples. In summary, the results of this study suggest that the current diagnostic standard procedure for polymicrobial UTI samples needs to be reassessed, and that E. coli present in polymicrobial UTI samples may pose an increased risk to human health

Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in Caenorhabditis elegans.

Oocyte meiotic spindles orient with one pole juxtaposed to the cortex to facilitate extrusion of chromosomes into polar bodies. In Caenorhabditis elegans, these acentriolar spindles initially orient parallel to the cortex and then rotate to the perpendicular orientation. To understand the mechanism of spindle rotation, we characterized events that correlated temporally with rotation, including shortening of the spindle in the pole-to pole axis, which resulted in a nearly spherical spindle at rotation. By analyzing large spindles of polyploid C. elegans and a related nematode species, we found that spindle rotation initiated at a defined spherical shape rather than at a defined spindle length. In addition, dynein accumulated on the cortex just before rotation, and microtubules grew from the spindle with plus ends outward during rotation. Dynactin depletion prevented accumulation of dynein on the cortex and prevented spindle rotation independently of effects on spindle shape. These results support a cortical pulling model in which spindle shape might facilitate rotation because a sphere can rotate without deforming the adjacent elastic cytoplasm. We also present evidence that activation of spindle rotation is promoted by dephosphorylation of the basic domain of p150 dynactin

Temperature Fluctuations driven by Magnetorotational Instability in Protoplanetary Disks

The magnetorotational instability (MRI) drives magnetized turbulence in sufficiently ionized regions of protoplanetary disks, leading to mass accretion. The dissipation of the potential energy associated with this accretion determines the thermal structure of accreting regions. Until recently, the heating from the turbulence has only been treated in an azimuthally averaged sense, neglecting local fluctuations. However, magnetized turbulence dissipates its energy intermittently in current sheet structures. We study this intermittent energy dissipation using high resolution numerical models including a treatment of radiative thermal diffusion in an optically thick regime. Our models predict that these turbulent current sheets drive order unity temperature variations even where the MRI is damped strongly by Ohmic resistivity. This implies that the current sheet structures where energy dissipation occurs must be well resolved to correctly capture the flow structure in numerical models. Higher resolutions are required to resolve energy dissipation than to resolve the magnetic field strength or accretion stresses. The temperature variations are large enough to have major consequences for mineral formation in disks, including melting chondrules, remelting calcium-aluminum rich inclusions, and annealing silicates; and may drive hysteresis: current sheets in MRI active regions could be significantly more conductive than the remainder of the disk.Comment: 16 pages, 13 figures, ApJ In Press, updated to match proof

A Preliminary Investigation into Geopolymer Cement Mortar’s Suitability for Providing Resilient Highway Solutions

Despite local and national road authorities striving to provide motorists with a durable and safe infrastructure environment, one in six UK roads are currently classed as being in poor condition. In terms of safety, Department for Transport statistics continue to report high numbers of road fatalities; 1,780 in 2015, representing a 3% increase from the previous year. As such, research focused on developing resilient and cost effective planned/preventative highway maintenance solutions remains highly topical. Reported in this paper is research aimed at developing high performance, low impact solutions for both highway repair and skid resistance enhancement. A metakaolin/alkali silicate-based geopolymer cementitious mix design investigation is initially reported, providing key fresh and mechanical material properties such as setting time and compressive/flexural strength. Using favourable mix designs, the paper presents an assessment of geopolymer cement mortar’s suitability as a highway repair material. To this end, wear and skidding resistance characteristics of potholes repaired with geopolymer cement concrete is reported, with initial findings suggesting excellent performance levels. Finally, the paper examines the potential use of a geopolymer cement mortar-based artificial aggregate as a cost-effective alternative to calcined bauxite for high friction surfacing applications. The results of this phase of the work show potential for geopolymer application, although ongoing optimisation research is required

Fast transcription rates of RNA polymerase II in human cells

Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min(-1). In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min(-1) points towards a wide dynamic range of RNAPII velocities in living cells

Plasma modeling for a nonsymmetric capacitive discharge driven by a nonsinusoidal radio frequency current

An analytical solution for the sheath dynamics of an asymmetrically driven capacitively coupled plasma is obtained under the assumptions of time-independent, collisionless ion motion, inertialess electrons, and uniform current density. Modeling is performed considering that the plasma is driven by a nonsinusoidal radio frequency (rf) current which can be resolved into a finite number of harmonic components. Together with different sheath parameters the equation for the bulk plasma impedance is also obtained to calculate the overall plasma impedance and the overall rf voltage. Assuming equal plate areas the solution for a symmetric discharge is also obtainable from this model. We have found that the even harmonic components of rf voltage and impedance are always present, even in a symmetric discharge. Experimental results are shown to be in qualitative agreement with the theoretical model. The values of normalized rf voltage and impedance harmonics assume lower values as the asymmetry of the plasma chamber decreases

Continuous data assimilation for global numerical weather prediction

A new configuration of the European Centre for Medium-Range Weather Forecasts (ECMWF) incremental 4D-Var data assimilation (DA) system is introduced which builds upon the quasi-continuous DA concept proposed in the mid-1990s. Rather than working with a fixed set of observations, the new 4D-Var configuration exploits the near-continuous stream of incoming observations by introducing recently arrived observations at each outer loop iteration of the assimilation. This allows the analysis to benefit from more recent observations. Additionally, by decoupling the start time of the DA calculations from the observational data cut-off time, real-time forecasting applications can benefit from more expensive analysis configurations that previously could not have been considered. In this work we present results of a systematic comparison of the performance of a Continuous DA system against that of two more traditional baseline 4D-Var configurations. We show that the quality of the analysis produced by the new, more continuous configuration is comparable to that of a conventional baseline that has access to all of the observations in each of the outer loops, which is a configuration not feasible in real-time operational numerical weather prediction. For real-time forecasting applications, the Continuous DA framework allows configurations which clearly outperform the best available affordable non-continuous configuration. Continuous DA became operational at ECMWF in June 2019 and led to significant 2 to 3% reductions in medium-range forecast root mean square errors, which is roughly equivalent to 2-3 hr of additional predictive skill.Peer reviewe

Analysis of the impact of dislocation distribution on the breakdown voltage of GaAs-based power varactor diodes

A synchrotron x-ray topography analysis of the impact of the distribution of defects/dislocations on the electrical performance of GaAs power varactor diodes was carried out. Diodes fabricated on or near Liquid Encapsulated Czochralski cellular dislocation networks in the substrate, which are also known to be rich in As precipitates near these cell walls, were observed to have reduced breakdown voltages (VBR). This is consistent with the possibility that the presence of space-charge cylinders surrounding these dislocations gives rise to reduced VBR if they thread a p-n junction; it is also in accord with the possibility that the As precipitates themselves can act as sites for local field enhancement, thus promoting premature avalanche breakdown