SAM 2 data user's guide

This document is intended to serve as a guide to the use of the data products from the Stratospheric Aerosol Measurement (SAM) 2 experiment for scientific investigations of polar stratospheric aerosols. Included is a detailed description of the Beta and Aerosol Number Density Archive Tape (BANAT), which is the SAM 2 data product containing the aerosol extinction data available for these investigations. Also included are brief descriptions of the instrument operation, data collection, processing and validation, and some of the scientific analyses conducted to date

SAGE 1 data user's guide

A guide for using the data products from the Stratospheric Aerosol and Gas Experiment 1 (SAGE 1) for scientific investigations of stratospheric chemistry related to aerosol, ozone, nitrogen dioxide, dynamics, and climate change is presented. A detailed description of the aerosol profile tape, the ozone profile tape, and the nitrogen dioxide profile tape is included. These tapes are the SAGE 1 data products containing aerosol extinction data and ozone and nitrogen dioxide concentration data for use in the different scientific investigations. Brief descriptions of the instrument operation, data collection, processing, and validation, and some of the scientific analyses that were conducted are also included

SAGE 3: A visible wavelength limb sounder

A brief description is presented for the SAGE 3 (Stratospheric Aerosol and Gas Experiment 3) instrument that has been selected to fly onboard the National Polar Platform 1 (NPOP 1) for the Earth Observational System (Eos) in 1996. The SAGE 3 instrument will perform earth limb sounding with the solar occultation technique measuring the ultraviolet (UV), the visible, and the near infrared (IR) wavelength solar radiation. The instrument will produce atmospheric data for the vertical distribution of aerosol, ozone, nitrogen dioxide, water vapor, and oxygen. The details of the instrument design, data flow, and processing requirements are discussed

Observations of atmospheric water vapor with the SAGE 2 instrument

The Stratospheric Aerosol and Gas Experiment 2 (SAGE 2) is discussed. The SAGE 2 instrument was a multichannel spectrometer that inferred the vertical distribution of water vapor, aerosols, nitrogen dioxide, and ozone by measuring the extinction of solar radiation at spacecraft sunrise/sunset. At altitudes above 20 km, the SAGE 2 and LIMS (Limb Infrared Monitor of the Stratosphere) data are in close agreement. The discrepancies below this altitude may be attributed to differences in the instruments' field of view and time of data acquisition

SAM-2 ground-truth plan: Correlative measurements for the Stratospheric Aerosol Measurement-2 (SAM 2) sensor on the Nimbus G satellite

The SAM-2 will fly aboard the Nimbus-G satellite for launch in the fall of 1978 and measure stratospheric vertical profiles of aerosol extinction in high latitude bands. The plan gives details of the location and times for the simultaneous satellite/correlative measurements for the nominal launch time, the rationale and choice of the correlative sensors, their characteristics and expected accuracies, and the conversion of their data to extinction profiles. The SAM-2 expected instrument performance and data inversion results are presented. Various atmospheric models representative of polar stratospheric aerosols are used in the SAM-2 and correlative sensor analyses

CELLULAR REQUIREMENTS FOR THE PRIMARY IN VITRO ANTIBODY RESPONSE TO DNP-FICOLL

The cellular requirements for the primary in vitro IgM and IgG response to dinitrophenyl-substituted Ficoll were examined. Neither thymus-derived lymphocytes nor macrophage-rich splenic adherent cells were required for anti-DNP antibody synthesis. DNP-Ficoll is therefore tentatively classified as a "thymic-independent" antigen

Real-time analysis of gene regulation by glucocorticoid hormones

There is increasing evidence that temporal factors are important in allowing cells to gain additional information from external factors, such as hormones and cytokines. We sought to discover how cell responses to glucocorticoids develop over time, and how the response kinetics vary according to ligand structure and concentration, and hence have developed a continuous gene transcription measurement system, based on an interleukin-6 (IL-6) luciferase reporter gene. We measured the time to maximal response, maximal response and integrated response, and have compared these results with a conventional, end point glucocorticoid bioassay. We studied natural glucocorticoids (corticosterone and cortisol), synthetic glucocorticoids (dexamethasone) and glucocorticoid precursors with weak, or absent bioactivity. We found a close correlation between half maximal effective concentration (EC50) for maximal response, and for integrated response, but with consistently higher EC50 for the latter. There was no relation between the concentration of ligand and the time to maximal response. A comparison between conventional end point assays and real-time measurement showed similar effects for dexamethasone and hydrocortisone, with a less effective inhibition of IL-6 seen with corticosterone. We profiled the activity of precursor steroids, and found pregnenolone, progesterone, 21-hydroxyprogesterone and 17-hydroxyprogesterone all to be ineffective in the real-time assay, but in contrast, progesterone and 21-hydroxyprogesterone showed an IL-6 inhibitory activity in the end point assay. Taken together, our data show how ligand concentration can alter the amplitude of glucocorticoid response, and also that a comparison between real-time and end point assays reveals an unexpected diversity of the function of glucocorticoid precursor steroids, with implications for human disorders associated with their overproduction

Effect of BMAP-28 Antimicrobial Peptides on Leishmania major Promastigote and Amastigote Growth: Role of Leishmanolysin in Parasite Survival

Protozoan parasites are the causative agent of much disease in tropical areas of the world. Currently, the control of these diseases is dependent on outdated drug treatment, with associated high toxicity and drug resistance. There is an urgent need for novel anti-parasitic therapies. One emerging anti-parasitic therapies is Host defence peptides (HDPs). Here we test the HDP BMAP-28 as an anti-leishmanial therapy against two lifecycle stages of Leishmania major, the promastigotes (insect infective form) and the intracellular amastigote (mammalian infective form). Two stereoisomers of BMAP-28, the D-amino acid form (D-BMAP-28) and the retro-inverso form (RI-BMAP-28), were also tested for anti-leishmanial activity. The BMAP-28 form (L-form) was susceptible to degradation by GP63, the metalloproteinase that covers the promastigotes cell surface. However, the BMAP-28 isomers, the D-form and RI-form were resistant, and therefore more potent against the promastigote parasite. Though other anti-leishmanial HDP studies focus on the promastigote form of the parasite, it is the mammalian infective form, the amastigote, which causes the disease symptoms. Here we demonstrate that BMAP-28 and its isomers D-BMAP-28 and RI-BMAP-28 are effective against the amastigote form of the parasite using a macrophage infection model. These findings show that BMAP-28 has excellent potential as a novel anti-leishmanial therapeutic

Enhanced Immune Responses by Skin Vaccination with Influenza Subunit Vaccine in Young Hosts

Skin has gained substantial attention as a vaccine target organ due to its immunological properties, which include a high density of professional antigen presenting cells (APCs). Previous studies have demonstrated the effectiveness of this vaccination route not only in animal models but also in adults. Young children represent a population group that is at high risk from influenza infection. As a result, this group could ben- efit significantly from influenza vaccine delivery approaches through the skin and the improved immune response it can induce. In this study, we compared the immune responses in young BALB/c mice upon skin delivery of influenza vaccine with vaccination by the conventional intramuscular route. Young mice that received 5 fLg of H1N1 A/Ca/07/09 influenza subunit vaccine using MN demonstrated an improved serum antibody response (IgG1 and IgG2a) when compared to the young IM group, accompanied by higher numbers of influenza-specific antibody secreting cells (ASCs) in the bone marrow. In addition, we observed increased activation of follicular helper T cells and formation of germinal centers in the regional lymph nodes in the MN immunized group, rapid clearance of the virus from their lungs as well as complete survival, compared with partial protection observed in the IM-vaccinated group. Our results support the hypothesis that influenza vaccine delivery through the skin would be beneficial for protecting the high-risk young population from influenza infection