Recreation of the terminal events in physiological integrin activation.

Increased affinity of integrins for the extracellular matrix (activation) regulates cell adhesion and migration, extracellular matrix assembly, and mechanotransduction. Major uncertainties concern the sufficiency of talin for activation, whether conformational change without clustering leads to activation, and whether mechanical force is required for molecular extension. Here, we reconstructed physiological integrin activation in vitro and used cellular, biochemical, biophysical, and ultrastructural analyses to show that talin binding is sufficient to activate integrin alphaIIbbeta3. Furthermore, we synthesized nanodiscs, each bearing a single lipid-embedded integrin, and used them to show that talin activates unclustered integrins leading to molecular extension in the absence of force or other membrane proteins. Thus, we provide the first proof that talin binding is sufficient to activate and extend membrane-embedded integrin alphaIIbbeta3, thereby resolving numerous controversies and enabling molecular analysis of reconstructed integrin signaling

Cell Attachment and Osteoinductive Properties of Tissue Engineered, Demineralized Bone Fibers for Bone Void Filling Applications

Demineralized bone matrices (DBMs) have been used in a wide variety of clinical applications involving bone repair. Ideally, DBMs should provide osteoinductive and osteoconductive properties, while offering versatile handling capabilities. With this, a novel fiber technology, LifeNet Health-Moldable Demineralized Fibers (L-MDF), was recently developed. Human cortical bone was milled and demineralized to produce L-MDF. Subsequently, the fibers were lyophilized and terminally sterilized using low-dose and low-temperature gamma irradiation. Using L929 mouse fibroblasts, L-MDF underwent cytotoxicity testing to confirm lack of a cytotoxic response. An alamarBlue assay and scanning electron microscopy demonstrated L-MDF supported the cellular function and attachment of bone-marrow mesenchymal stem cells (BM-MSCs). Using an enzyme-linked immunosorbent assay, L-MDF demonstrated BMP-2 and 7 levels similar to those reported in the literature. In vivo data from an athymic mouse model implanted with L-MDF demonstrated the formation of new bone elements and blood vessels. This study showed that L-MDF have the necessary characteristics of a bone void filler to treat osseous defects

Hyposplenism and gastrointestinal diseases:significance and mechanisms

Open Access via the Karger Agreement This work was funded in part by Wellcome Trust Grant number 0934847/Z/10/Z.Peer reviewedPublisher PD

Speech rhythm auto-recurrence is negatively linked to quality of mental-health counseling interactions

We explored use of Recurrence Quantification Analysis (RQA) of speech rhythm data from mental-health counseling sessions for prediction of quality of psychotherapy. Time-series of inter-syllable intervals (ISIs) were extracted from 239 counseling sessions conducted by 12 therapists who repeatedly interacted with 30 clients. We found a negative association between recurrence metrics and client-rated session quality and a negative link between percent of laminarity and therapist-rated session quality, after controlling for self-reported client depression and distress measures and duration of speech sound within a session. Placing value on reduced recurrence in patterns of ISIs, and especially reduced degree of a dyadic system remaining in the same speech-rhythm pattern may be indicative of a desire for variation in content and strategies of client-therapist interaction. These exploratory findings point to the possibility of RQA-based automated systems to capture the ‘footprint’ of the non-verbal dynamic that is indicative of successful mental-health counseling

Identifying Young Brown Dwarfs Using Gravity-Sensitive Spectral Features

We report the initial results of the Brown Dwarf Spectroscopic Survey Gravity Project, to study gravity sensitive features as indicators of youth in brown dwarfs. Low-resolution (R~2000) J-band and optical (R~1000) observations using NIRSPEC and LRIS at the W.M. Keck Observatory reveal transitions of TiO, VO, K I, Na I, Cs I, Rb I, CaH, and FeH. By comparing these features in late-type giants and in old field dwarfs we show that they are sensitive to the gravity (g = GM/R^2) of the object. Using low-gravity spectral signatures as age indicators, we observed and analyzed J-band and optical spectra of two young brown dwarfs, G 196-3B (20-300 Myr) and KPNO Tau-4 (1-2 Myr), and two possible low mass brown dwarfs in the sigma Orionis cluster (3-7 Myr). We report the identification of the phi bands of TiO near 1.24 microns and the A-X band of VO near 1.18 microns together with extremely weak J-band lines of K I in KPNO-Tau4. This is the first detection of TiO and VO in the J-band in a sub-stellar mass object. The optical spectrum of KPNO-Tau4 exhibits weak K I and Na I lines, weak absorption by CaH, and strong VO bands, also signatures of a lower gravity atmosphere. G 196-3B shows absorption features in both wavelength regions like those of KPNO-Tau4 suggesting that its age and mass are at the lower end of published estimates. Whereas sigma Ori 51 appears to be consistent with a young sub-stellar object, sigma Ori 47 shows signatures of high gravity most closely resembling an old L1.5/L0, and can not be a member of the sigma Orionis cluster.Comment: 14 pages, 4 figures. To appear in the January 10, 2004 issue of the Astrophysical Journa

The NIRSPEC Brown Dwarf Spectroscopic Survey II: High-Resolution J-Band Spectra of M, L and T Dwarfs

We present a sequence of high resolution (R~20,000 or 15 km/s) infrared spectra of stars and brown dwarfs spanning spectral types M2.5 to T6. Observations of 16 objects were obtained using eight echelle orders to cover part of the J-band from 1.165-1.323 micron with NIRSPEC on the Keck II telescope. By comparing opacity plots and line lists, over 200 weak features in the J-band are identified with either FeH or H2O transitions. Absorption by FeH attains maximum strength in the mid-L dwarfs, while H2O absorption becomes systematically stronger towards later spectral types. Narrow resolved features broaden markedly after the M to L transition. Our high resolution spectra also reveal that the disappearance of neutral Al lines at the boundary between M and L dwarfs is remarkably abrupt, presumably because of the formation of grains. Neutral Fe lines can be traced to mid-L dwarfs before Fe is removed by condensation. The neutral potassium (K I) doublets that dominate the J-band have pressure broadened wings that continue to broaden from ~50 km/s (FWHM) at mid-M to ~500 km/s at mid-T. In contrast however, the measured pseudo-equivalent widths of these same lines reach a maximum in the mid-L dwarfs. The young L2 dwarf, G196-3B, exhibits narrow potassium lines without extensive pressure-broadened wings, indicative of a lower gravity atmosphere. Kelu-1AB, another L2, has exceptionally broad infrared lines, including FeH and H2O features, confirming its status as a rapid rotator. In contrast to other late T objects, the peculiar T6 dwarf 2MASS 0937+29 displays a complete absence of potassium even at high resolution, which may be a metallicity effect or a result of a cooler, higher-gravity atmosphere.Comment: 53 pages, 21 figures, data will be available at http://www.astro.ucla.edu/~mclean/BDSSarchive

Discovery of a Very Young Field L Dwarf, 2MASS J01415823-4633574

While following up L dwarf candidates selected photometrically from the Two Micron All Sky Survey, we uncovered an unusual object designated 2MASS J01415823-4633574. Its optical spectrum exhibits very strong bands of vanadium oxide but abnormally weak absorptions by titanium oxide, potassium, and sodium. Morphologically such spectroscopic characteristics fall intermediate between old, field early-L dwarfs (log(g)~5) and very late M giants (log(g)~0), leading us to favor low gravity as the explanation for the unique spectral signatures of this L dwarf. Such a low gravity can be explained only if this L dwarf is much lower in mass than a typical old field L dwarf of similar temperature and is still contracting to its final radius. These conditions imply a very young age. Further evidence of youth is found in the near-infrared spectrum, including a triangular-shaped H-band continuum reminiscent of young brown dwarf candidates discovered in the Orion Nebula Cluster. Using the above information along with comparisons to brown dwarf atmospheric and interior models, our current best estimate is that this L dwarf has an age of 1-50 Myr and a mass of 6-25 M_Jupiter. The location of 2MASS 0141-4633 on the sky coupled with a distance estimate of ~35 pc and the above age estimate suggests that this object may be a brown dwarf member of either the 30-Myr-old Tucana/Horologium Association or the ~12-Myr-old beta Pic Moving Group.Comment: Accepted for publication in the 10 March 2006 issue (volume 639) of the Astrophysical Journa

The NIRSPEC Brown Dwarf Spectroscopic Survey. I. Low-Resolution Near-Infrared Spectra

We present the first results of a near-infrared (0.96-2.31 micron) spectroscopic survey of M, L, and T dwarfs obtained with NIRSPEC on the Keck II telescope. Our new survey has a resolving power of R = 2000 and is comprised of two major data sets: 53 J-band (1.14-1.36 micron) spectra covering all spectral types from M6 to T8 with at least two members in each spectral subclass (wherever possible), and 25 flux-calibrated spectra from 1.14 to 2.31 microns for most spectral classes between M6 and T8. Sixteen of these 25 objects have additional spectral coverage from 0.96-1.14 microns to provide overlap with optical spectra. Spectral flux ratio indexes for prominent molecular bands are derived and equivalent widths (EWs) for several atomic lines are measured. We find that a combination of four H2O and two CH4 band strengths can be used for spectral classification. Weak (EW~1-2 angstrom) atomic lines of Al I and Ca I disappear at the boundary between M and L types.Comment: 60 pages, 25 figures. To appear in the Astrophysical Journal Vol 596. Received 2003 March 31; accepted 2003 June 20. Web site at http://www.astro.ucla.edu/~mclean/BDSSarchiv

High Resolution Infrared Imaging and Spectroscopy of the Pistol Nebula: Evidence for Ejection

We present new NICMOS/HST infrared images and CGS4/UKIRT Br-alpha (4.05 um) spectroscopy of the Pistol Star and its associated nebula, finding strong evidence to support the hypothesis that the Pistol Nebula was ejected from the Pistol Star. The Pa-alpha NICMOS image shows that the nebula completely surrounds the Pistol Star, although the line intensity is much stronger on its northern and western edges. The Br-alpha spectra show the classical ring-like signature of quasi-spherical expansion, with weak blueshifted emission (V_max approx -60 km/s) and strong redshifted emission (V_max approx +10 km/s), where the velocities are with respect to the velocity of the Pistol Star; further, the redshifted emission appears to be "flattened" in the position-velocity diagram. These data suggest that the nebula was ejected from the star several thousand years ago, with a velocity between the current terminal velocity of the stellar wind (95 km/s) and the present expansion velocity of gas in the outer shell of the nebula (60 km/s). The Pa-alpha image reveals several emission-line stars in the region, including two newly-identified emission-line stars north of the Pistol Star with spectral types earlier than WC8 (T_eff > 50,000 K). The presence of these stars, the morphology of the Pa-alpha emission, and the velocity field in the gas suggest that the side of the nebula furthest from us is approaching, and being ionized by, the hot stars of the Quintuplet, and that the highest velocity redshifted gas has been decelerated by winds from the Quintuplet stars. We also discuss the possibility that the nebular gas might be magnetically confined by the ambient magnetic field delineated by the nearby nonthermal filaments.Comment: Figure 1 is included as a JPG file. Figure 1 and 2 also available at ftp://quintup.astro.ucla.edu/pistol2

The Evolution of the Stellar Hosts of Radio Galaxies

We present new near-infrared images of z>0.8 radio galaxies from the flux-limited 7C-III sample of radio sources for which we have recently obtained almost complete spectroscopic redshifts. The 7C objects have radio luminosities about 20 times fainter than 3C radio galaxies at a given redshift. The absolute magnitudes of the underlying host galaxies and their scale sizes are only weakly dependent on radio luminosity. Radio galaxy hosts at z~2 are significantly brighter than the hosts of radio-quiet quasars at similar redshifts and the model AGN hosts of Kauffmann & Haehnelt (2000). There is no evidence for strong evolution in scale size, which shows a large scatter at all redshifts. The hosts brighten significantly with redshift, consistent with the passive evolution of a stellar population that formed at z>~3. This scenario is consistent with studies of host galaxy morphology and submillimeter continuum emission, both of which show strong evolution at z>~2.5. The lack of a strong ``redshift cutoff'' in the radio luminosity function to z>4 suggests that the formation epoch of the radio galaxy host population lasts >~1Gyr from z>~5 to z~3. We suggest these facts are best explained by models in which the most massive galaxies and their associated AGN form early due to high baryon densities in the centres of their dark matter haloes.Comment: To appear in A