Siderophilic Cyanobacteria for the Development of Extraterrestrial Photoautotrophic Biotechnologies

In-situ production of consumables (mainly oxygen) using local resources (In-Situ Resource Utilization-ISRU) will significantly facilitate current plans for human exploration and settlement of the solar system, starting with the Moon. With few exceptions, nearly all technologies developed to date have employed an approach based on inorganic chemistry. None of these technologies include concepts for integrating the ISRU system with a bioregenerative life support system and a food production system. Therefore, a new concept based on the cultivation of cyanobacteria (CB) in semi-closed biogeoreactor, linking ISRU, a biological life support system, and food production, has been proposed. The key feature of the biogeoreactor is to use lithotrophic CB to extract many needed elements such as Fe directly from the dissolved regolith and direct them to any technological loop at an extraterrestrial outpost. Our studies showed that siderophilic (Fe-loving) CB are capable to corrode lunar regolith stimulants because they secrete chelating agents and can tolerate [Fe] up to 1 mM. However, lunar and Martian environments are very hostile (very high UV and gamma-radiation, extreme temperatures, deficit of water). Thus, the selection of CB species with high potential for extraterrestrial biotechnologies that may be utilized in 15 years must be sponsored by NASA as soon as possible. The study of the genomes of candidate CB species and the metagenomes of the terrestrial environments which they inhabit is critical to make this decision. Here we provide preliminary results about peculiarities of the genomes of siderophilic CB revealed by analyzing the genome of siderophilic cyanobacterium JSC-1 and the metagenome of iron depositing hot spring (IDHS) Chocolate Pots (Yellowstone National Park, Wyoming, USA). It has been found that IDHS are richer with ferrous iron than the majority of hot springs around the world. Fe2+ is known to increase the magnitude of oxidative stress in prokaryotes through so called Fenton reaction. It is not surprising therefore that the CB inhabiting IDHS have larger sets of the proteins involved in the maintenance of Fe homeostasis and oxidative stress protection than non-siderophilic CB. This finding combined with our earlier results about the ability of some siderophilic CB to utilize chemical elements released from analogs of lunar and Martian regolith make them the most advanced candidates to be employed in advanced extraterrestrial biotechnologies

Supporting reinterpretation in computer-aided conceptual design

This paper presents research that aims to inform the development of computational tools that better support design exploration and idea transformation - key objectives in conceptual design. Analyses of experimental data from two fields - product design and architecture - suggest that the interactions of designers with their sketches can be formalised according to a finite number of generalised shape rules defined within a shape grammar. Such rules can provide a basis for the generation of alternative design concepts and they have informed the development of a prototype shape synthesis system that supports dynamic reinterpretation of shapes in design activity. The notion of 'sub-shapes' is introduced and the significance of these to perception, recognition and the development of emergent structures is discussed. The paper concludes with some speculation on how such a system might find application in a range of design fields

Sampling Distributions of Random Electromagnetic Fields in Mesoscopic or Dynamical Systems

We derive the sampling probability density function (pdf) of an ideal localized random electromagnetic field, its amplitude and intensity in an electromagnetic environment that is quasi-statically time-varying statistically homogeneous or static statistically inhomogeneous. The results allow for the estimation of field statistics and confidence intervals when a single spatial or temporal stochastic process produces randomization of the field. Results for both coherent and incoherent detection techniques are derived, for Cartesian, planar and full-vectorial fields. We show that the functional form of the sampling pdf depends on whether the random variable is dimensioned (e.g., the sampled electric field proper) or is expressed in dimensionless standardized or normalized form (e.g., the sampled electric field divided by its sampled standard deviation). For dimensioned quantities, the electric field, its amplitude and intensity exhibit different types of Bessel $K$ sampling pdfs, which differ significantly from the asymptotic Gauss normal and $\chi^{(2)}_{2p}$ ensemble pdfs when $\nu$ is relatively small. By contrast, for the corresponding standardized quantities, Student $t$, Fisher-Snedecor $F$ and root-$F$ sampling pdfs are obtained that exhibit heavier tails than comparable Bessel $K$ pdfs. Statistical uncertainties obtained from classical small-sample theory for dimensionless quantities are shown to be overestimated compared to dimensioned quantities. Differences in the sampling pdfs arising from de-normalization versus de-standardization are obtained.Comment: 12 pages, 15 figures, accepted for publication in Phys. Rev. E, minor typos correcte

Effects of weak anchoring on C1 and C2 chevron structures

We present a theoretical study of the effect of weak anchoring on the transition between C1 and C2 chevron structures in smectic C liquid crystals. We employ a continuum theory which allows for variable cone, azimuthal and layer tilt angles. Equilibrium profiles for the director cone and azimuthal angles in the C1 and C2 states are calculated from the standard Euler-Lagrange minimisation of the total energy of the system. By comparing the total energies of the C1 and C2 states we can determine the globally stable chevron profile and calculate the critical temperature for the C1-C2 transition, which depends on anchoring strength and pretilt angle variations

Atmospheric, Evolutionary, and Spectral Models of the Brown Dwarf Gliese 229 B

Theoretical spectra and evolutionary models that span the giant planet--brown dwarf continuum have been computed based on the recent discovery of the brown dwarf, Gliese 229 B. A flux enhancement in the 4--5 micron window is a universal feature from Jovian planets to brown dwarfs. We confirm the existence of methane and water in Gl 229 B's spectrum and find its mass to be 30 to 55 Jovian masses. Although these calculations focus on Gliese 229 B, they are also meant to guide future searches for extra-solar giant planets and brown dwarfs.Comment: 8 pages, plain TeX, plus four postscript figures, gzipped and uuencoded, accepted for Scienc

Beagle to the Moon: nn experiment package to measure polar ice and volatiles in permanently shadowed areas or beneath the lunar surface

The Beagle Science Package is a flight qualified set of instruments which should be deployed to the lunar surface to answer the questions about water and volatiles present in permanently shadowed regions and/or beneath the surface

Using the Generic Synteny Browser (GBrowse_syn)

Genome Browsers are software that allow the user to view genome annotations in the context of a reference sequence, such as a chromosome, contig, scaffold, etc. The Generic Genome Browser (GBrowse) is an open-source genome browser package developed as part of the Generic Model Database Project (see UNIT ; Stein et al., 2002). The increasing number of sequenced genomes has led to a corresponding growth in the field of comparative genomics, which requires methods to view and compare multiple genomes. Using the same software framework as GBrowse, the Generic Synteny Browser (GBrowse_syn) allows the comparison of colinear regions of multiple genomes using the familiar GBrowse-style Web page. Like GBrowse, GBrowse_syn can be configured to display any organism, and is currently the synteny browser used for model organisms such as C. elegans (WormBase; http://www.wormbase.org; see UNIT 1.8) and Arabidopsis (TAIR; http://www.arabidopsis.org; see UNIT 1.1). GBrowse_syn is part of the GBrowse software package and can be downloaded from the Web and run on any Unix-like operating system, such as Linux, Solaris, or MacOS X. GBrowse_syn is still under active development. This unit will cover installation and configuration as part of the current stable version of GBrowse (v. 1.71)

Signatures of Pseudoscalar Photon Mixing in CMB Radiation

We model the effect of photon and ultra-light pseudoscalar mixing on the propagation of electromagnetic radiation through the extragalactic medium. The medium is modelled as a large number of magnetic domains, uncorrelated with one another. We obtain an analytic expression for the different Stokes parameters in the limit of small mixing angle. The different Stokes parameters are found to increase linearly with the number of domains. We also verify this result by direct numerical simulations. We use this formalism to estimate the effect of pseudoscalar-photon mixing on the Cosmic Microwave Background (CMB) polarization. We impose limits on the model parameters by the CMB observations. We find that the currently allowed parameter range admits a CMB circular polarization up to order $10^{-7}$.Comment: 17 pages, 5 figure

Child support judgments: comparing public policy to the public's policy

Any child support regime necessarily makes policy choices about how parental income should be shared between the two parental households. Those choices involve balancing the claims of the child, the claims of the custodial parent for help with the expense of providing for the child, and the claims of the support obligor for autonomy in deciding how to spend his own earnings. That balancing task is complicated by the fact that the child and the custodial parent necessarily share a living standard, so that any child support transfer, large or small, will unavoidably benefit the custodial parent as well as the child. This article reports the findings of an empirical study designed to reveal how the British public believe this balance should be struck. It then compares the public’s preferred policies to the policy choices implicit in the current UK child support schedule. It concludes that there are important gaps between the two, and recommends that consideration be given to amending the current UK law to better align it with the public’s values on these matters