14 research outputs found
[Book Review of] \u3cem\u3eEuthanasia, Ethics and Public Policy: An Argument Against Legalisation\u3c/em\u3e, by John Keown
Report of the AACP Task Force on Patient-Centered Medical Homes and Accountable Care Organizations
A task force was convened by the American Association of Colleges of Pharmacy (AACP) to provide rationale for academic pharmacy engagement with organizations/institutions providing new models of team-based patient care designed to improve patient access, care quality, and affordable care. Descriptions of new care models, such as patient centered medical homes (PCMHs) and accountable care organizations (ACO), were included as well as descriptions of existing partnerships with academic pharmacy
Bovine serum eliminates rapid nonspecific toxic reactions during bioassay of stored fish for Clostridium botulinum toxin
Report of the AACP task force on patient-centered medical homes and accountable care organizations
Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics
Biologic drugs are typically manufactured
in mammalian host cells,
and it is critical from a drug safety and efficacy perspective to
detect and remove host cell proteins (HCPs) during production. This
is currently achieved with sets of polyclonal antibodies (pAbs), but
these suffer from critical shortcomings because their composition
is inherently undefined, and they cannot detect nonimmunogenic HCPs.
In this work, we report a high-throughput screening and array-based
binding characterization strategy that we employed to generate a set
of aptamers that overcomes these limitations to achieve sensitive,
broad-spectrum detection of HCPs from the widely used Chinese hamster
ovary (CHO) cell line. We identified a set of 32 DNA aptamers that
achieve better sensitivity than a commercial pAb reagent set and can
detect a comparable number of HCPs over a broad range of isoelectric
points and sizes. Importantly, these aptamers detect multiple contaminants
that are known to be responsible for therapeutic antibody degradation
and toxicity in patients. Because HCP aptamer reagents are sequence-defined
and chemically synthesized, we believe they may enable safer production
of biologic drugs, and this strategy should be broadly applicable
for the generation of HCP detection reagents for other cell lines