TRPV4: Molecular Conductor of a Diverse Orchestra

Transient receptor potential vanilloid type 4 (TRPV4) is a calcium-permeable nonselective cation channel, originally described in 2000 by research teams led by Schultz ( Nat Cell Biol 2: 695 –702, 2000) and Liedtke ( Cell 103: 525–535, 2000). TRPV4 is now recognized as being a polymodal ionotropic receptor that is activated by a disparate array of stimuli, ranging from hypotonicity to heat and acidic pH. Importantly, this ion channel is constitutively expressed and capable of spontaneous activity in the absence of agonist stimulation, which suggests that it serves important physiological functions, as does its widespread dissemination throughout the body and its capacity to interact with other proteins. Not surprisingly, therefore, it has emerged more recently that TRPV4 fulfills a great number of important physiological roles and that various disease states are attributable to the absence, or abnormal functioning, of this ion channel. Here, we review the known characteristics of this ion channel's structure, localization and function, including its activators, and examine its functional importance in health and disease.</jats:p

Physiology and Pharmacology Ca V 3.1 T-Type Ca 2þ Channels Contribute to Myogenic Signaling in Rat Retinal Arterioles

PURPOSE. Although L-type Ca 2þ channels are known to play a key role in the myogenic reactivity of retinal arterial vessels, the involvement of other types of voltage-gated Ca 2þ channels in this process remains unknown. In the present study we have investigated the contribution of T-type Ca 2þ channels to myogenic signaling in arterioles of the rat retinal microcirculation. METHODS. Confocal immunolabeling of whole-mount preparations was used to investigate the localization of Ca V 3.1-3 channels in retinal arteriolar smooth muscle cells. T-type currents and the contribution of T-type channels to myogenic signaling were assessed by whole-cell patchclamp recording and pressure myography of isolated retinal arteriole segments. RESULTS. Strong immunolabeling for Ca V 3.1 was observed on the plasma membrane of retinal arteriolar smooth muscle cells. In contrast, no expression of Ca V 3.2 or Ca V 3.3 could be detected in retinal arterioles, although these channels were present on glial cell end-feet surrounding the vessels and retinal ganglion cells, respectively. TTA-A2-sensitive T-type currents were recorded in retinal arteriolar myocytes with biophysical properties distinct from those of the L-type currents present in these cells. Inhibition of T-type channels using TTA-A2 or ML-218 dilated isolated, myogenically active, retinal arterioles. CONCLUSIONS. Ca V 3.1 T-type Ca 2þ channels are functionally expressed on arteriolar smooth muscle cells of retinal arterioles and play an important role in myogenic signaling in these vessels. The work has important implications concerning our understanding of the mechanisms controlling blood flow autoregulation in the retina and its disruption during ocular disease

ISBS 2018 AUCKLAND CONFERENCE WORKSHOPS PROGRAMME

FORCEDECKS WORKSHOP - COMMERCIALISATION & FUNDING by Philip Graham Smith This workshop aims to improve your ability to attract and create commercialisation and funding opportunities. This workshop will challenge delegates to identify their real areas of expertise and consider ways in which they can attract and create funding opportunities. The aim is to help academics of all ages to focus their expertise, to manage their time more effectively and to explore new avenues to make their careers more rewarding, fulfilling and hopefully less stressful. Having been a former Head of Department and Associate Head of School (Business & Engagement), Dr Graham-Smith has been in the trenches and acknowledges the increasing demands and pressures of working in academia. The workshop will help delegates to strip back the various aspects of their roles, and to examine ways in which their teaching, research, consultancy and funding expectations can be managed successfully. Phil will be reflective on his own career and share experiences of working in academia, professional sport and private industry. SPRINZ WORKSHOP - ORAL PRESENTATION FEEDBACK by Joshua McGeown, Gillian Weir, Professor Mike McGuigan and SPRINZ PhD students This workshop aims to help you engaging your audience during your ISBS presentation. This workshop aims to provide delegates with tips and feedback as to how best present their research for the ISBS 2018 congress. This interactive workshop will help delegates to learn how to distill and communicate complex ideas, structure your narrative and how to best visualize your data. Participants are encouraged to bring their ISBS presentations to practice and receive constructive feedback. NZ HERALD WORKSHOP - HOW TO WORK WITH THE MEDIA TO AMPLIFY YOUR WORK by Dylan Cleaver, Editor at large with the New Zealand Herald This workshop will help delegates be able to interact with media to be able to amplify their work. Never before has there been so much attention given to the injury toll in elite sport, with the spotlight firmly centered on head injuries and the potential for long-term cognitive damage to those afflicted. With so much important research being done in the field of sports injury, it is important to know how to work with the media to highlight it. This workshop aims to give a brief overview of the fast-changing modern media landscape. It will offer advice as to how to establish contacts in the media and how to use those contacts wisely. It will demonstrate how to get your key messages across using simple language, without dumbing down the issue. It will traverse ethical issues and, finally, what to do when the message goes wrong. Attendees will use the lessons learnt from the examples, to workshop during the session how they can work with media to amplify their work. WORKSHOP - JAPAN COLLABORATION by Sayumi Iwamoto, Erika Ikeda, Ryu Nagahara, and Aaron Uthoff Do you want to share your experience with other researchers who are keen to conduct international research collaboration? The workshop will share experiences and key tips to enable successfully working together. “There are many positives with working with Japanese researchers, but the one that stands out the most to me is their willingness to share knowledge and lend a helping hand.” (Aaron Uthoff) AUT ENGINEERING WORKSHOP - AI CHALLENGES by Boris Bacic & Russell Pears from Auckland University of Technology Engineering School This workshop will help you to consider pushing your boundaries of biomechanics and sport science by embracing artificial intelligence (Dr Boris Bačić and Assoc. Prof Russel Pears, Auckland University of Technology, NZ). Pushing the boundaries of biomechanics and sport science also means embracing artificial intelligence (AI) to advance and augment ways in which sport is coached, played, promoted, broadcasted and commercialised. Technologies capable of capturing human motion enable the advancement of research and can create strategic differences in elite sport, which is reflected by their increasing presence in the growing market of sport gadgets, exergames and rehabilitation technologies. Data-driven machine-learning AI approaches have the potential to provide insights from data, find patterns in specific contexts, generate knowledge, validate expert’s common-sense rules, and offload support decisions and automate cognitive activities. The workshop will provide a theoretical introduction and a set of analytical and model-designing visual tools for getting started. For those interested in Matlab or other languages, code samples will be provided. The participants will be able to use free open source software alternatives as part of hands-on exercises in a supervised lab. SPRINGER WORKSHOP - WHAT MAKES A SUCCESSFUL PAPER – AN EDITOR’S PERSPECTIVE by Steve McMillan from Springer’s Sports Medicine journal This workshop will help delegates increase their likelihood of success in publishing in journals such as Sports Medicine. From a compelling cover letter to a concise conclusion, Sports Medicine’s Co-Editor in Chief, Steve McMillan, will provide an editor’s perspective on what makes a successful paper. Sports Medicine receives over 600 submissions a year and can publish only a quarter of these … How do the editors decide which manuscripts to send to peer review? Which manuscripts survive peer review? What details are essential to enable readers to best understand your research and allow for potential replication? What information is required from an ethical perspective? Why do word counts matter anyway?! This interactive workshop will guide you on how to produce an impressive manuscript and increase your chances of getting published in a reputable journal. NORAXON WORKSHOP - ELECTROMYOGRAPHY IN SPORTS PERFORMANCE by Coleman Bessert and Erin Feser from NORAXON. Noraxon USA (www.noraxon.com) will be hosting a workshop on electromyography (EMG) use in sports performance settings. “You will be able to develop a better understanding of how EMG fits into an athlete monitoring program or research investigation by learning what can, and cannot, be determined with EMG data and reporting. Participants will see hands-on use of precision EMG systems and biomechanics analysis software with practical, sport-specific examples.” Erin Feser , Director of Education for Noraxon USA

Narrative writing, reading and cognitive processes in middle childhood: what are the links?

This study investigated the relationship between measures of reading and writing, and explored whether cognitive measures known to be related to reading ability were also associated with writing performance in middle childhood. Sixty-Four children, aged between 8 years 9 months and 11 years 9 months, took part in a battery of writing, reading, and cognitive ability tasks. Reading fluency emerged as having a strong relationship to written language performance, after controlling for age and verbal reasoning. While children with reading difficulties were weak at spelling accuracy, they were otherwise found to produce written compositions of similar quality to typical readers. Boys produced less written text than girls, but did not demonstrate weaker written language abilities. Collectively the results demonstrate that writing skills can be separated into transcription and composition processes, and highlight the need for further research on the relationship between reading fluency and children’s writing