Estimating the Effects Weather, Dry Matter Intake, and Body Weight on Daily Water Intake in Weaned Calves

Water is an essential nutrient and is required for growth, metabolism, lactation, and reproduction. However, water requirements for beef cattle have not been studied extensively since the 1950’s. The genetic potential for beef cattle has changed considerably since then. With the change in genetics, beef producers have observed increases in average daily gain and final body weight. The need for updated water requirements for beef cattle has also been exacerbated by global climate variability, drought conditions, and the need for efficient use of water resources. Given the increased productivity of cattle today relative to those of decades ago, increased water requirements are almost certain. Further research must be conducted to determine how those requirements have changed

Towards Spectral Classification of L and T Dwarfs: Infrared and Optical Spectroscopy and Analysis

We present 0.6-2.5um, R~400 spectra of twenty-seven cool, low luminosity stars and substellar objects. Based on these and previously published spectra we develop a preliminary spectral classification system for L and T dwarfs. For late L and T types the classification system is based entirely on four spectral indices in the 1-2.5um interval. Two of these indices are derived from water absorption bands at 1.15um and 1.4um, the latter of which shows a smooth increase in depth through the L and T sequences and can be used to classify both spectral types. The other two indices make use of methane absorption features in the H and K bands, with the K band index also applicable to mid to late L dwarfs. Continuum indices shortward of 1um used by previous authors to classify L dwarfs are found to be useful only through mid L subclasses. We employ the 1.5um water index and the 2.2um methane index to complete the L classification through L9.5 and to link the new system with a modified version of the 2MASS ``Color-d'' index. By correlating the depths of the methane and water absorption features, we establish a T spectral sequence from types T0 to T8, based on all four indices, which is a smooth continuation of the L sequence. We reclassify two 2MASS L8 dwarfs as L9 and L9.5 and identify one SDSS object as L9. In the proposed system methane absorption appears in the K band approximately at L8, two subclasses earlier than its appearance in the H band. The L and T spectral classes are distinguished by the absence and presence, respectively, of H band methane absorption.Comment: 40 pages, 14 figures, to be published in Ap.J., Jan 1, 200

Online and clicker quizzing on jargon terms enhances definition-focused but not conceptually focused biology exam performance

Mastery of jargon terms is an important part of student learning in biology and other science, technology, engineering, and mathematics domains. In two experiments, we investigated whether prelecture quizzes enhance memory for jargon terms, and whether that enhanced familiarity can facilitate learning of related concepts that are encountered during subsequent lectures and readings. Undergraduate students enrolled in neuroanatomy and physiology courses completed 10-minute low-stakes quizzes with feedback on jargon terms either online (experiment 1) or using in-class clickers (experiment 2). Quizzes occurred before conventional course instruction in which the terms were used. On exams occurring up to 12 weeks later, we observed improved student performance on questions that targeted memory of previously quizzed jargon terms and their definitions relative to questions on terms that were not quizzed. This pattern occurred whether those questions were identical (experiment 1) or different (experiment 2) from those used during quizzing. Benefits of jargon quizzing did not consistently generalize, however, to exam questions that assessed conceptual knowledge but not necessarily jargon knowledge. Overall, this research demonstrates that a brief and easily implemented jargon-quizzing intervention, deliverable via Internet or in-class platforms, can yield substantial improvements in students\u27 course-relevant scientific lexica, but does not necessarily impact conceptual learning

Low-level copper concentration measurements in silicon wafers using trace-element accelerator mass spectrometry

This article discusses low-level copper concentration measurements in silicon wafers using trace-element accelerator mass spectrometry

Engineering the Controlled Assembly of Filamentous Injectisomes in E. coli K-12 for Protein Translocation into Mammalian Cells.

Bacterial pathogens containing type III protein secretion systems (T3SS) assemble large needle-like protein complexes in the bacterial envelope, called injectisomes, for translocation of protein effectors into host cells. The application of these molecular syringes for the injection of proteins into mammalian cells is hindered by their structural and genomic complexity, requiring multiple polypeptides encoded along with effectors in various transcriptional units (TUs) with intricate regulation. In this work, we have rationally designed the controlled expression of the filamentous injectisomes found in enteropathogenic Escherichia coli (EPEC) in the nonpathogenic strain E. coli K-12. All structural components of EPEC injectisomes, encoded in a genomic island called the locus of enterocyte effacement (LEE), were engineered in five TUs (eLEEs) excluding effectors, promoters and transcriptional regulators. These eLEEs were placed under the control of the IPTG-inducible promoter Ptac and integrated into specific chromosomal sites of E. coli K-12 using a marker-less strategy. The resulting strain, named synthetic injector E. coli (SIEC), assembles filamentous injectisomes similar to those in EPEC. SIEC injectisomes form pores in the host plasma membrane and are able to translocate T3-substrate proteins (e.g., translocated intimin receptor, Tir) into the cytoplasm of HeLa cells reproducing the phenotypes of intimate attachment and polymerization of actin-pedestals elicited by EPEC bacteria. Hence, SIEC strain allows the controlled expression of functional filamentous injectisomes for efficient translocation of proteins with T3S-signals into mammalian cells

Alumosilicate ceramic proppants based on natural refractory raw materials

The sintering-strengthening effect of the additions of the highly ferrous bauxite (with Fe[2]O[3] content of 20-25 % in the calcined state) in the compositions with refractory clays was established. It was found that in the temperature range 1350-1500°C the additions of bauxite in amounts of 10-40% have a fluxing effect due to the iron oxide introduced with bauxite in compositions with clay. An increasing the bauxite additive in the amount of 50-70% ensures its strengthening effect by increasing the total content of the mullite of the prismatic habit in the firing products of composites with clay. Preliminary clay and bauxite calcination at 900 °С and an increase in the content of bauxite additive up to 50-70% in compositions with clay allow to produce aluminosilicate proppants with a bulk density of 1.62-1.65 g/сm{3} and compressive strength up to 52 MPa

Vector meson production and nucleon resonance analysis in a coupled-channel approach for energies m_N < sqrt(s) < 2 GeV II: photon-induced results

We present a nucleon resonance analysis by simultaneously considering all pion- and photon-induced experimental data on the final states gamma N, pi N, 2 pi N, eta N, K Lambda, K Sigma, and omega N for energies from the nucleon mass up to sqrt(s) = 2 GeV. In this analysis we find strong evidence for the resonances P_{31}(1750), P_{13}(1900), P_{33}(1920), and D_{13}(1950). The omega N production mechanism is dominated by large P_{11}(1710) and P_{13}(1900) contributions. In this second part we present the results on the photoproduction reactions and the electromagnetic properties of the resonances. The inclusion of all important final states up to sqrt(s) = 2 GeV allows for estimates on the importance of the individual states for the GDH sum rule.Comment: 41 pages, 26 figures, discussion extended, typos corrected, references updated, to appear in Phys. Rev.

The host metabolite D-serine contributes to bacterial niche specificity through gene selection

Escherichia coli comprise a diverse array of both commensals and niche-specific pathotypes. The ability to cause disease results from both carriage of specific virulence factors and regulatory control of these via environmental stimuli. Moreover, host metabolites further refine the response of bacteria to their environment and can dramatically affect the outcome of the host–pathogen interaction. Here, we demonstrate that the host metabolite, D-serine, selectively affects gene expression in E. coli O157:H7. Transcriptomic profiling showed exposure to D-serine results in activation of the SOS response and suppresses expression of the Type 3 Secretion System (T3SS) used to attach to host cells. We also show that concurrent carriage of both the D-serine tolerance locus (dsdCXA) and the locus of enterocyte effacement pathogenicity island encoding a T3SS is extremely rare, a genotype that we attribute to an ‘evolutionary incompatibility’ between the two loci. This study demonstrates the importance of co-operation between both core and pathogenic genetic elements in defining niche specificity

Responses to gestational weight management guidance: a thematic analysis of comments made by women in online parenting forums

Background: The National Institute for Health and Clinical Excellence (NICE) published guidance on weight management in pregnancy in July 2010[1], and this received considerable press coverage across a range of media. This offered an opportunity to examine how gestational weight management guidance was received by UK women. Methods: A thematic analysis was conducted of 400 posts made in UK-based parenting internet forums in the week following the publication of the NICE guidance. This allowed us to examine the naturally occurring comments from 202 women who posted about the guidance on public forums. Results: Three main themes were identified and explored: i) Perceived control/responsibility ii) Risk perception iii) Confused messages. Conclusions: Women differed in their perceptions of the level of control that they had over being overweight with some feeling responsible and motivated to maintain a healthy lifestyle. Others felt there were multiple factors influencing their weight issues beyond their control. There were reports of feeling guilty about the impact of weight on the growing baby and experiencing significant obesity stigma from the public and health professionals. Information about the risks of overweight and obesity in pregnancy were difficult messages for women to hear, and for health professionals to deliver. Women reported being confused by the messages that they received. Health messages need to be delivered sensitively to women, and health professionals need support and training to do this. Risk information should always be accompanied with clear advice and support to help women to manage their weight in pregnancy. Keywords: internet-mediated research, gestational weight gain, parenting forums, NICE, women, views, risk perception</p