7,234 research outputs found
Automatic signal range selector for metering devices Patent
Voltage range selection apparatus for sensing and applying voltages to electronic instruments without loading signal sourc
Adaptive multigrid algorithm for the lattice Wilson-Dirac operator
We present an adaptive multigrid solver for application to the non-Hermitian
Wilson-Dirac system of QCD. The key components leading to the success of our
proposed algorithm are the use of an adaptive projection onto coarse grids that
preserves the near null space of the system matrix together with a simplified
form of the correction based on the so-called gamma_5-Hermitian symmetry of the
Dirac operator. We demonstrate that the algorithm nearly eliminates critical
slowing down in the chiral limit and that it has weak dependence on the lattice
volume
Development and evaluation of lessons for class and group situations in grade one
Thesis (Ed.M.)--Boston Universit
Exploring the Dust Content of Galactic Winds with Herschel. I. NGC 4631
We present a detailed analysis of deep far-infrared observations of the
nearby edge-on star-forming galaxy NGC 4631 obtained with the Herschel Space
Observatory. Our PACS images at 70 and 160 um show a rich complex of filaments
and chimney-like features that extends up to a projected distance of 6 kpc
above the plane of the galaxy. The PACS features often match extraplanar
Halpha, radio-continuum, and soft X-ray features observed in this galaxy,
pointing to a tight disk-halo connection regulated by star formation. On the
other hand, the morphology of the colder dust component detected on larger
scale in the SPIRE 250, 350, and 500 um data matches the extraplanar H~I
streams previously reported in NGC 4631 and suggests a tidal origin. The PACS
70/160 ratios are elevated in the central ~3.0 kpc region above the nucleus of
this galaxy (the "superbubble"). A pixel-by-pixel analysis shows that dust in
this region has a higher temperature and/or an emissivity with a steeper
spectral index (beta > 2) than the dust in the disk, possibly the result of the
harsher environment in the superbubble. Star formation in the disk seems
energetically insufficient to lift the material out of the disk, unless it was
more active in the past or the dust-to-gas ratio in the superbubble region is
higher than the Galactic value. Some of the dust in the halo may also have been
tidally stripped from nearby companions or lifted from the disk by galaxy
interactions.Comment: Accepted for publication in The Astrophysical Journa
Optimizing end-labeled free-solution electrophoresis by increasing the hydrodynamic friction of the drag-tag
We study the electrophoretic separation of polyelectrolytes of varying
lengths by means of end-labeled free-solution electrophoresis (ELFSE). A
coarse-grained molecular dynamics simulation model, using full electrostatic
interactions and a mesoscopic Lattice Boltzmann fluid to account for
hydrodynamic interactions, is used to characterize the drag coefficients of
different label types: linear and branched polymeric labels, as well as
transiently bound micelles.
It is specifically shown that the label's drag coefficient is determined by
its hydrodynamic size, and that the drag per label monomer is largest for
linear labels. However, the addition of side chains to a linear label offers
the possibility to increase the hydrodynamic size, and therefore the label
efficiency, without having to increase the linear length of the label, thereby
simplifying synthesis. The third class of labels investigated, transiently
bound micelles, seems very promising for the usage in ELFSE, as they provide a
significant higher hydrodynamic drag than the other label types.
The results are compared to theoretical predictions, and we investigate how
the efficiency of the ELFSE method can be improved by using smartly designed
drag-tags.Comment: 32 pages, 11 figures, submitted to Macromolecule
Analysis of transmitted HIV-1 drug resistance using 454 ultra-deep-sequencing and the DeepChek(®)-HIV system.
Introduction
Next-generation sequencing (NGS) is capable of detecting resistance-associated mutations (RAMs) present at frequencies of 1% or below. Several studies have found that baseline low-frequency RAMs are associated with failure to first-line HAART. One major limitation to the expansion of this technology in routine diagnostics is the complexity and laboriousness integral to bioinformatics analysis. DeepChek (ABL, TherapyEdge) is a CE-marked software that allows automated analysis and resistance interpretation of NGS data.
Objective
To evaluate the use of 454 ultra-deep-sequencing (Roche® 454, Life Sciences; 454-UDS) and DeepChek for routine baseline resistance testing in a clinical diagnostic laboratory.
Methods
107 newly diagnosed HIV-1-infected patients (subtypes: A, n=9; B, n=52; C, n=21; D, n=2; F, n=3; G, n=1; CRF01, n=7; CRF02, n=7; CRF06, n=1; CRF07, n=1; CRF10, n=1 and unassigned complex, n=2) with a median plasma viral load of 88,727 copies/mL (range: 1380–2,143,543) were tested by 454-UDS and Sanger sequencing for the detection of protease and reverse transcriptase RAMs. In addition, integrase RAMs were investigated in 57 of them. Sequence analysis and resistance interpretation were performed using DeepChek applying 1% and 20% thresholds for variant detections; filters applied were comparison between Sanger and 454-UDS, and Stanford and IAS list for resistance interpretation.
Results
The time elapsed from generation of raw 454 data (between 2,000–5,000 sequences/sample) to elaboration of a resistance report was approximately 10 minutes per sample, equivalent to the time required for the same process using Sanger sequencing. Four patients (3.7%) showed baseline resistance by Sanger and 454-UDS at frequencies above 20%, which affected both NRTIs (n=2) and NNRTIs (n=2). In addition, 12 patients (11.2%) showed transmitted drug resistance (TDR) by 454-UDS at frequencies below 20% affecting NRTIs (n=9), NNRTIs (n=7) and PIs (n=2). Integrase resistance was not detected at baseline by 454-UDS or Sanger sequencing.
Conclusions
DeepChek allowed easy and rapid analysis and interpretation of NGS data, thus facilitating the incorporation of this technology in routine diagnostics. The use of NGS considerably increased the detection rates of TDR to NRTI, NNRTIs and PIs. No transmitted resistance to integrase inhibitors was found in our population by Sanger sequencing or UDS
Fluctuations in viscous fingering
Our experiments on viscous (Saffman-Taylor) fingering in Hele-Shaw channels
reveal finger width fluctuations that were not observed in previous
experiments, which had lower aspect ratios and higher capillary numbers Ca.
These fluctuations intermittently narrow the finger from its expected width.
The magnitude of these fluctuations is described by a power law, Ca^{-0.64},
which holds for all aspect ratios studied up to the onset of tip instabilities.
Further, for large aspect ratios, the mean finger width exhibits a maximum as
Ca is decreased instead of the predicted monotonic increase.Comment: Revised introduction, smoothed transitions in paper body, and added a
few additional minor results. (Figures unchanged.) 4 pages, 3 figures.
Submitted to PRE Rapi
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