12 research outputs found
The influence of bacteria on multitrophic interactions among plants, psyllids, and pathogen
Reprint of “Early testosterone modulated sex differences in behavioral outcome following neonatal hypoxia ischemia in rats”
Computational neurology: computational modeling approaches in Dementia
Dementia is a collection of symptoms associated with impaired cognition and impedes everyday normal functioning. Dementia, with Alzheimer’s disease constituting its most common type, is highly complex in terms of etiology and pathophysiology. A more quantitative or computational attitude towards dementia research, or more generally in neurology, is becoming necessary – Computational Neurology. We provide a focused review of some computational approaches that have been developed and applied to the study of dementia, particularly Alzheimer’s disease. Both mechanistic modeling and data-driven, including AI or machine learning, approaches are discussed. Linkage to clinical decision support systems for dementia diagnosis will also be discussed
Combined Effect of Water Activity, pH and Sub-optimal Temperature on Growth and Enterotoxin Production of Staphylococcus aureus
Characterization of Citrus tristeza virus isolates from grapefruit (Citrus paradisi Macf.) accessions of Citrus Active Germplasm Bank Caracterização de isolados do vĂrus da tristeza dos citros de acessos de pomelos (Citrus paradisi Macf.) do Banco Ativo de Germoplasma de Citros
Citrus tristeza virus (CTV) isolates from 35 grapefruit accessions belonging to Citrus Active Germplasm Bank of the "Instituto AgronĂ´mico de Campinas" located at the "Centro APTA Citros Sylvio Moreira", CordeirĂłpolis, SĂŁo Paulo state, Brazil, were characterized and evaluated through symptoms in the trees, biological indexing, immunological diagnosis with different monoclonal antibodies and SSCP analysis (single-strand conformation polymorphism) of the coat protein gene. Symptomatology indicated that, in general, the group of plants with smaller canopy volume and severe stem pitting differed significantly from the group that presented greater vegetative development and mild to moderate stem pitting. However, the isolates from most of the accessions induced mild reaction on Mexican lime. The serological evaluation through the DAS-ELISA using monoclonal antibodies did not reveal any association between virus titer in the plant tissue and symptoms. The reaction with different monoclonal antibodies and the distinct electrophoresis patterns obtained through SSCP showed that there is a high degree of diversity among the isolates that infect these grapefruit accessions. High complexity within the same isolate was also observed in the SSCP profiles. This finding indicates that the CTV isolates from these plants are a complex mixture of CTV haplotypes. Similar SSCP banding patterns were observed among some plants with strong stem pitting symptoms, and among some plants with weak or moderate stem pitting symptoms.<br>Isolados do vĂrus da tristeza dos citros (CTV) de 35 acessos de pomelos que fazem parte do Banco Ativo de Germoplasma de Citros, localizado no Centro APTA Citros Sylvio Moreira, CordeirĂłpolis, SĂŁo Paulo, Brasil, pertencente ao Instituto AgronĂ´mico de Campinas (IAC), foram caracterizados atravĂ©s dos sintomas observados nas árvores, indexação biolĂłgica, diagnĂłstico imunolĂłgico e análise SSCP (polimorfismo de conformação de fita simples) do gene da proteĂna do capsĂdeo. O grupo de plantas que, em geral, apresentou menor volume de copa e severo sintoma de canelura diferenciou-se significativamente do grupo com maior desenvolvimento vegetativo e fraco a moderado sintoma de canelura. No entanto, a maioria dos isolados de CTV das plantas de ambos os grupos induziu fraca reação em limĂŁo galego e nenhuma relação entre tĂtulos do vĂrus nos tecidos e sintomatologia foi observada na avaliação sorolĂłgica conduzida por DAS-ELISA. A reação com diferentes anticorpos monoclonais e os distintos padrões eletroforĂ©ticos obtidos por SSCP demonstraram que há uma grande diversidade entre os isolados de CTV que infectam os acessos de pomelos. Alta complexidade de bandas dentro de um mesmo isolado foi tambĂ©m observada nos perfis SSCP, demonstrando que cada isolado Ă© constituĂdo por uma mistura de diferentes haplĂłtipos de CTV. Padrões SSCP semelhantes foram observados entre algumas plantas com fortes sintomas de caneluras e entre algumas plantas com sintomas fracos ou moderados de caneluras