6 research outputs found
Development, application and computational analysis of high-dimensional fluorescent antibody panels for single-cell flow cytometry
Single-cell profiling defines the prognostic benefit of CD39 high tissue resident memory CD8+ T cells in luminal-like breast cancer
Contains fulltext :
238682.pdf (Publisher’s version ) (Open Access
Circulating mucosal-associated invariant T cells identify patients responding to anti-PD-1 therapy
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235601.pdf (Publisher’s version ) (Open Access
IRF4 instructs effector Treg differentiation and immune suppression in human cancer
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220923.pdf (Publisher’s version ) (Closed access
Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans
T cell memory relies on the generation of antigen-specific progenitors with stem-like properties. However, the identity of these progenitors has remained unclear, precluding a full understanding of the differentiation trajectories that underpin the heterogeneity of antigen-experienced T cells. We used a systematic approach guided by single-cell RNA-sequencing data to map the organizational structure of the human CD8+ memory T cell pool under physiological conditions. We identified two previously unrecognized subsets of clonally, epigenetically, functionally, phenotypically and transcriptionally distinct stem-like CD8+ memory T cells. Progenitors lacking the inhibitory receptors programmed death-1 (PD-1) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) were committed to a functional lineage, whereas progenitors expressing PD-1 and TIGIT were committed to a dysfunctional, exhausted-like lineage. Collectively, these data reveal the existence of parallel differentiation programs in the human CD8+ memory T cell pool, with potentially broad implications for the development of immunotherapies and vaccines