Transfusion effect of random donor platelet and single donor platelet in thrombocytopenic patients at tertiary care hospital of South Gujarat

Background: Platelet transfusion plays a key role in therapy for the patients with thrombocytopenia. Superiority of Single donor platelet (SDP) over Random donor platelet (RDP) transfusions is largely assumed, but unproven. Platelet Rich Plasma-Platelet concentrate (PRP-PC) and Apheresis-PC were prepared and their therapeutic efficacy were assessed in thrombocytopenic patients.Methods: This study included 60 transfusion episodes consisting of 30 SDP and 30 RDP (147units of RDP). The post transfusion efficacy of transfused platelets was assessed at 1 hour and 24 hours by corrected count increment (CCI) and percentage recovery (PR). Paired ‘t’-test was used for statistical analysis and a probability of p<0.05 was used to reject null hypothesis.Results: The mean platelet dose of SDP (n=30) and RDP (n=30) was 2.86±1.05 x 1011 and 2.36±0.54 x 1011 respectively. The mean platelet increments of SDP at 1 hour and 24 hours were 38±18.1 x 103/μl and 37.3±20.7x 103/μl. The mean platelet increments of RDP at 1 hour and 24 hours were 28.5±11.4 x 103/μl and 26 ±11.6 x 103/μl. The mean CCI of SDP at 1hour and 24 hours were 21.4 ±7.3 x 103/μl and 20.8±7.4 x 103/μl respectively. The mean CCI of RDP at 1hour and 24 hours were 18.5±6.3x 103/μl and 17.4±7.6 x 103/μl respectively.Conclusions: Post-transfusion increments were significantly higher in patients who received SDP as compared to RDP, but the CCI and PR were comparable in both groups of patients

Prevalence and trends of transfusion transmitted infections among blood donors of blood bank attached to government hospital of South Gujarat, India

Background: Though the blood transfusion is lifesaving, it is never risk free and carries potential risk for transfusion transmitted infections (TTIs).The objective of present study is to assess the prevalence and trend of TTIs among blood donors in the blood bank attached to tertiary level government hospital and its comparison with national data and other studies of different regions.Methods: Retrospective cross-sectional study was done including healthy blood donor of 18-65 years from May 2011 to April 2016. TTIs testing were done with 3rd generation ELISA for HIV, HBV and HCV and HIV testing was also done with 4th generation ELISA from the year 2014 onwards. Syphilis screening was done either by RPR, TPHA strip test or ELISA and Malaria parasite was tested either by peripheral smear or rapid test.Results: From 40971 donors in study, 550 (1.34%) donors were sero-reactive from which 400 (0.98%) donors were reactive for HBV, 67 (0.16%) were for syphilis, 40 (0.098%) were for HCV, 33 (0.081%) were reactive for HIV and 10 (0.024%) were malaria positive. During the study period four donors showed co infection for HIV and HBV. Most common age group to be sero reactive in the present study is 21-30 years of age (43.09% of total).Conclusions: Prevalence of TTIs in the present study was lower as compare to other studies of the India. The reasons behind this finding may be better pre-donation counseling, better life style, higher proportion of voluntary blood donation (> 99%) and high pre-donation deferral rate (11.17%)

Prevalence and trends of transfusion transmitted infections among blood donors of blood bank attached to government hospital of South Gujarat, India

Background: Though the blood transfusion is lifesaving, it is never risk free and carries potential risk for transfusion transmitted infections (TTIs).The objective of present study is to assess the prevalence and trend of TTIs among blood donors in the blood bank attached to tertiary level government hospital and its comparison with national data and other studies of different regions.Methods: Retrospective cross-sectional study was done including healthy blood donor of 18-65 years from May 2011 to April 2016. TTIs testing were done with 3rd generation ELISA for HIV, HBV and HCV and HIV testing was also done with 4th generation ELISA from the year 2014 onwards. Syphilis screening was done either by RPR, TPHA strip test or ELISA and Malaria parasite was tested either by peripheral smear or rapid test.Results: From 40971 donors in study, 550 (1.34%) donors were sero-reactive from which 400 (0.98%) donors were reactive for HBV, 67 (0.16%) were for syphilis, 40 (0.098%) were for HCV, 33 (0.081%) were reactive for HIV and 10 (0.024%) were malaria positive. During the study period four donors showed co infection for HIV and HBV. Most common age group to be sero reactive in the present study is 21-30 years of age (43.09% of total).Conclusions: Prevalence of TTIs in the present study was lower as compare to other studies of the India. The reasons behind this finding may be better pre-donation counseling, better life style, higher proportion of voluntary blood donation (&gt; 99%) and high pre-donation deferral rate (11.17%)

Customized Deep Learning Classifier for Detection of Acute Lymphoblastic Leukemia Using Blood Smear Images

Acute lymphoblastic leukemia (ALL) is a rare type of blood cancer caused due to the overproduction of lymphocytes by the bone marrow in the human body. It is one of the common types of cancer in children, which has a fair chance of being cured. However, this may even occur in adults, and the chances of a cure are slim if diagnosed at a later stage. To aid in the early detection of this deadly disease, an intelligent method to screen the white blood cells is proposed in this study. The proposed intelligent deep learning algorithm uses the microscopic images of blood smears as the input data. This algorithm is implemented with a convolutional neural network (CNN) to predict the leukemic cells from the healthy blood cells. The custom ALLNET model was trained and tested using the microscopic images available as open-source data. The model training was carried out on Google Collaboratory using the Nvidia Tesla P-100 GPU method. Maximum accuracy of 95.54%, specificity of 95.81%, sensitivity of 95.91%, F1-score of 95.43%, and precision of 96% were obtained by this accurate classifier. The proposed technique may be used during the pre-screening to detect the leukemia cells during complete blood count (CBC) and peripheral blood tests

Six-year multicenter study on short-term peripheral venous catheters-related bloodstream infection rates in 204 intensive care units of 57 hospitals in 19 cities of India: International Nosocomial Infection Control Consortium (INICC) findings

•We report peripheral venous catheters (PVC)-related BSI rates from 2013 to 2019.•We collected prospective data from 204 ICUs in 57 hospitals in 19 cities of India.•We followed 7,513 ICU patients for 296,893 bed-days and 295,795 PVC-days.•We identified 863 PVC-related BSIs, amounting to a rate of 2.91/1,000 PVC-days. Short-term peripheral venous catheters-related bloodstream infections (PVCR-BSIs) rates have not been systematically studied in developing countries, and data on their incidence by number of device-days are not available. Prospective, surveillance study on PVCR-BSI conducted from September 1, 2013 to May 31, 2019 in 204 intensive care units (ICUs), members of the International Nosocomial Infection Control Consortium (INICC), from 57 hospitals in 19 cities of India. We applied US INICC definition criteria and reported methods using the INICC Surveillance Online System. We followed 7,513 ICU patients for 296,893 bed-days and 295,795 short term peripheral venous catheter (PVC)-days. We identified 863 PVCR-BSIs, amounting to a rate of 2.91/1,000 PVC-days. Mortality in patients with PVC but without PVCR-BSI was 4.14%, and 11.59% in patients with PVCR-BSI. The length of stay in patients with PVC but without PVCR-BSI was 4.13 days, and 5.9 days in patients with PVCR-BSI. The micro-organism profile showed 68% of gram negative bacteria: Escherichia coli (23%), Klebsiella spp (15%), Pseudomonas aeruginosa (5%), and others. The predominant gram-positive bacteria were Staphylococcus aureus (10%). PVCR-BSI rates found in our ICUs were much higher than rates published from industrialized countries. Infection prevention programs must be implemented to reduce the incidence of PVCR-BSIs

Six-year study on peripheral venous catheter-associated BSI rates in 262 ICUs in eight countries of South-East Asia: International Nosocomial Infection Control Consortium findings

Short-term peripheral venous catheter-associated bloodstream infection rates have not been systematically studied in Asian countries, and data on peripheral venous catheter-associated bloodstream infections incidence by number of short-term peripheral venous catheter days are not available. Prospective, surveillance study on peripheral venous catheter-associated bloodstream infections conducted from 1 September 2013 to 31 May 2019 in 262 intensive care units, members of the International Nosocomial Infection Control Consortium, from 78 hospitals in 32 cities of 8 countries in the South-East Asia Region: China, India, Malaysia, Mongolia, Nepal, Philippines, Thailand, and Vietnam. For this research, we applied definition and criteria of the CDC NHSN, methodology of the INICC, and software named INICC Surveillance Online System. We followed 83,295 intensive care unit patients for 369,371 bed-days and 376,492 peripheral venous catheter-days. We identified 999 peripheral venous catheter-associated bloodstream infections, amounting to a rate of 2.65/1000 peripheral venous catheter-days. Mortality in patients with peripheral venous catheter but without peripheral venous catheter-associated bloodstream infections was 4.53% and 12.21% in patients with peripheral venous catheter-associated bloodstream infections. The mean length of stay in patients with peripheral venous catheter but without peripheral venous catheter-associated bloodstream infections was 4.40 days and 7.11 days in patients with peripheral venous catheter and peripheral venous catheter-associated bloodstream infections. The microorganism profile showed 67.1% were Gram-negative bacteria: (22.9%), spp (10.7%), (5.3%), spp. (4.5%), and others (23.7%). The predominant Gram-positive bacteria were (11.4%). Infection prevention programs must be implemented to reduce the incidence of peripheral venous catheter-associated bloodstream infections