2,454 research outputs found

    Farm-level data integration: future problems and consequences for public and private structures

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    One of the outcomes of the EC-FP7 project “Future Farm” was showing the need of INTEGRATION, something that PROGIS has been doing for 15 years. Within the whole sector agriculture–forestry-environment-risk management there is an enormous need for integration that is not available yet, because of on side the existing admin-sector-structures plus on the other side diverse public and/or private interests with opposite directions and in many cases the not streamlined interest of ALL involved parties. On the other hand we have the nature that is fully integrated and should be managed by us! Nothing happens without being related to something else within the nature. We have to be more aware of this and have also to understand that ICT will be the driver of integration as data and based on it these information is necessary and urgently needed for public and for private structures. We can do it separately, doing things in parallel and multiple times with multiple costs and reduced results. The other option is to cooperate on an integrative model!Commons, farm management, valuation of land, ICT, Farm Management,

    Translocation and insertion of precursor proteins into isolated outer membranes of mitochondria

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    Nuclear-encoded proteins destined for mitochondria must cross the outer or both outer and inner membranes to reach their final sub- mitochondrial locations. While the inner membrane can translocate preproteins by itself, it is not known whether the outer membrane also contains an endogenous protein translocation activity which can function independently of the inner membrane. To selectively study the protein transport into and across the outer membrane of Neurospora crassa mitochondria, outer membrane vesicles were isolated which were sealed, in a right-side-out orientation, and virtually free of inner membranes. The vesicles were functional in the insertion and assembly of various outer membrane proteins such as porin, MOM19, and MOM22. Like with intact mitochondria, import into isolated outer membranes was dependent on protease-sensitive surface receptors and led to correct folding and membrane integration. The vesicles were also capable of importing a peripheral component of the inner membrane, cytochrome c heme lyase (CCHL), in a receptor-dependent fashion. Thus, the protein translocation machinery of the outer mitochondrial membrane can function as an independent entity which recognizes, inserts, and translocates mitochondrial preproteins of the outer membrane and the intermembrane space. In contrast, proteins which have to be translocated into or across the inner membrane were only specifically bound to the vesicles, but not imported. This suggests that transport of such proteins involves the participation of components of the intermembrane space and/or the inner membrane, and that in these cases the outer membrane translocation machinery has to act in concert with that of the inner membrane

    Cryopreserved Corneal Tissue: A Practical Guide to Its Use

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    What we will explain in detail is the technique we have developed to actually use cryopreserved tissue in the operating room. Anyone who has followed the development of this type of transplant material has heard and read how important it is to follow exactly certain steps, but nowhere in the literature is there a guide as to how, in practice, the different steps are to be carried out. We know of no other procedure in ophthalmology where absolute adherence to the strictest set of guidelines is absolutely necessary

    Evidence for a magnesium-insensitive membrane resistance increase during NMDA-induced depolarizations in rat neocortical neurons in vitro

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    The responses of rat neocortical neurons in vitro to iontophoretically applied N-methyl-d-aspartate (NMDA) were investigated by means of intracellular recording in the presence and absence of extracellular magnesium ions (Mg2+). At Mg2+-concentrations of 1.3 mM the neurons responded with a depolarization accompanied by an increase in membrane resistance. Upon removal of Mg2+ the NMDA-induced depolarization was markedly potentiated. However, even in neurons recorded from slices which were incubated in a Mg2+-free solution for 3–7 h, the NMDA response was still associated with a resistance increase, suggesting that the voltage-dependence of the NMDA-activated conductance is not exclusively determined by Mg2+

    FutureFarm vision

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    This paper defines the first version of a vision of Future Farming project and also a knowledge management system used by European farms which will be designed and developed by the Future Farm project. An important part of the vision is a definition of external drivers and their influence on farm business in future. Paper is looking on a situation in three periods: short (2013), middle (2020) and long-term (2030). Our vision expects that the farming system will continuously converge to the situation of two types of farm: an industrial farm, which will guarantee both the food safety and the food security for European citizens, and multifunctional farms focused on environment protection. The recommendation proposes an architecture based on communication of interoperable services, so called Service Oriented Architecture (SOA), for easy integration of different levels and components of farm management.Farming, external drivers, future vision, knowledge management, SOA, Farm Management,

    Inclusion of proteins into isolated mitochondrial outer membrane vesicles

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    Nuclei embedded in an electron gas

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    The properties of nuclei embedded in an electron gas are studied within the relativistic mean-field approach. These studies are relevant for nuclear properties in astrophysical environments such as neutron-star crusts and supernova explosions. The electron gas is treated as a constant background in the Wigner-Seitz cell approximation. We investigate the stability of nuclei with respect to alpha and beta decay. Furthermore, the influence of the electronic background on spontaneous fission of heavy and superheavy nuclei is analyzed. We find that the presence of the electrons leads to stabilizing effects for both α\alpha decay and spontaneous fission for high electron densities. Furthermore, the screening effect shifts the proton dripline to more proton-rich nuclei, and the stability line with respect to beta decay is shifted to more neutron-rich nuclei. Implications for the creation and survival of very heavy nuclear systems are discussed.Comment: 35 pages, latex+ep

    Newton's method and Baker domains

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    We show that there exists an entire function f without zeros for which the associated Newton function N(z)=z-f(z)/f'(z) is a transcendental meromorphic functions without Baker domains. We also show that there exists an entire function f with exactly one zero for which the complement of the immediate attracting basin has at least two components and contains no invariant Baker domains of N. The second result answers a question of J. Rueckert and D. Schleicher while the first one gives a partial answer to a question of X. Buff.Comment: 6 page

    Formation and evolution of dwarf galaxies in the CDM Universe

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    We first review the results of the tidal stirring model for the transformation of gas-rich dwarf irregulars into dwarf spheroidals, which turns rotationally supported stellar systems into pressure supported ones. We emphasize the importance of the combined effect of ram pressure stripping and heating from the cosmic ultraviolet background in removing the gas and converting the object into a gas poor system as dSphs. We discuss how the timing of infall of dwarfs into the primary halo determines the final mass-to-light ratio and star formation history. Secondly we review the results of recent cosmological simulations of the formation of gas-rich dwarfs. These simulations are finally capable to produce a realistic object with no bulge, an exponential profile and a slowly rising rotation curve. The result owes to the inclusion of an inhomogeneous ISM and a star formation scheme based on regions having the typical density of molecular cloud complexes. Supernovae-driven winds become more effective in such mode, driving low angular momentum baryons outside the virial radius at high redshift and turning the dark matter cusp into a core. Finally we show the first tidal stirring experiments adopting dwarfs formed in cosmological simulations as initial conditions. The latter are gas dominated and have have turbulent thick gaseous and stellar disks disks that cannot develop strong bars, yet they are efficiently heated into spheroids by tidal shocks.Comment: 14 pages, 4 Figures, o appear in the proceedings of the CRAL conference, Lyon, June 2010, "A Universe of Dwarf Galaxies", eds. Philippe Prugniel & Mina Koleva; EDP Sciences in the European Astronomical Society Publications Series. (invited talk

    Des systèmes d'aide à la décision pour l'agriculture familiale

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