The Effect of Ascorbic Acid on Proliferation of Vascular Smooth Muscle Cells and Intimal Hyperplasia

Objective: Proliferation of smooth muscle cells leading to intimal hyperplasia developing at vascular intervention sites plays a major role in the development of restenosis. Ascorbic acid is a potent anti-oxidant with antiproliferative properties. The aim of this study was to investigate such effect of ascorbic acid on intimal hyperplasia. Material and Methods: Twenty-one male white New Zealand rabbits weighing 2-3 kg were selected. The animals were allocated to three groups each consisting of seven rabbits. Group 1 was the sham group. Group 2 was the control group and Group 3 consisted of rabbits receiving ascorbic acid. The right carotid arteries of the subjects in Groups 2 and 3 were transected and re-anastomosed. A daily dose of 100 mg ascorbic acid per kg body weight was administered intraperitoneally for 14 days to rabbits in Group 3. Rabbits in Group 2 were not subject to any pharmaceutical agent. All the subjects were sacrificed at the end of postoperative day 28. Their right carotid arteries were resected and were subject to histopathologic examination for smooth muscle cell proliferation and intimal hyperplasia. The arterial segments were fixed in 10% buffered formalin solution until the time of histological analysis. Each vessel was sectioned serially in 2-mm increments from the prepared paraffin blocks. Sections of 5 μm thickness were stained with hematoxylin and eosin (H&E) and Masson Trichrome. The diameters of vessels and their luminal areas, and the areas of tunica intima and tunica media were measured with 10X magnification. The thicknesses of tunica intima and tunica media were measured with 20X magnification. The morphometric measurements were then compared between groups. Results: Intimal thickness and intimal area were significantly lower in Group 1 when compared with the other groups (p=0.004, p=0.003). In Group 3, the ratios of tunica intima thickness/tunica media thickness and area of tunica intima/area of tunica media were significantly lower than those of Group 2 (p=0.015, p=0.046). Conclusion: Ascorbic acid reduces the intimal hyperplasia developing after vascular anastomoses. © 2013 by Türkiye Klinikleri

The effect of ascorbic acid on proliferation of vascular smooth muscle cells and intimal hyperplasia Askorbik Asidin Anastomoz Sonrasi Gelisen Vasküler Düz Kas Hücre Proliferasyonu ve Intimal Hiperplazi Üzerine Etkisi: Tavsan Modeli

Objective: Proliferation of smooth muscle cells leading to intimal hyperplasia developing at vascular intervention sites plays a major role in the development of restenosis. Ascorbic acid is a potent anti-oxidant with antiproliferative properties. The aim of this study was to investigate such effect of ascorbic acid on intimal hyperplasia. Material and Methods: Twenty-one male white New Zealand rabbits weighing 2-3 kg were selected. The animals were allocated to three groups each consisting of seven rabbits. Group 1 was the sham group. Group 2 was the control group and Group 3 consisted of rabbits receiving ascorbic acid. The right carotid arteries of the subjects in Groups 2 and 3 were transected and re-anastomosed. A daily dose of 100 mg ascorbic acid per kg body weight was administered intraperitoneally for 14 days to rabbits in Group 3. Rabbits in Group 2 were not subject to any pharmaceutical agent. All the subjects were sacrificed at the end of postoperative day 28. Their right carotid arteries were resected and were subject to histopathologic examination for smooth muscle cell proliferation and intimal hyperplasia. The arterial segments were fixed in 10% buffered formalin solution until the time of histological analysis. Each vessel was sectioned serially in 2-mm increments from the prepared paraffin blocks. Sections of 5 μm thickness were stained with hematoxylin and eosin (H&E) and Masson Trichrome. The diameters of vessels and their luminal areas, and the areas of tunica intima and tunica media were measured with 10X magnification. The thicknesses of tunica intima and tunica media were measured with 20X magnification. The morphometric measurements were then compared between groups. Results: Intimal thickness and intimal area were significantly lower in Group 1 when compared with the other groups (p=0.004, p=0.003). In Group 3, the ratios of tunica intima thickness/tunica media thickness and area of tunica intima/area of tunica media were significantly lower than those of Group 2 (p=0.015, p=0.046). Conclusion: Ascorbic acid reduces the intimal hyperplasia developing after vascular anastomoses. © 2013 by Türkiye Klinikleri

Consensus Report on Patient Blood Management in Cardiac Surgery by Turkish Society of Cardiovascular Surgery (TSCVS), Turkish Society of Cardiology (TSC), and Society of Cardio-Vascular-Thoracic Anaesthesia and Intensive Care (SCTAIC)

Anemia, transfusion and bleeding independently increase the risk of complications and mortality in cardiac surgery. The main goals of patient blood management are to treat anemia, prevent bleeding, and optimize the use of blood products during the perioperative period. The benefit of this program has been confirmed in many studies and its utilization is strongly recommended by professional organizations. This consensus report has been prepared by the authors who are the task members appointed by the Turkish Society of Cardiovascular Surgery, Turkish Society of Cardiology (TSC), and Society of Cardio-Vascular-Thoracic Anaesthesia and Intensive Care to raise the awareness of patient blood management. This report aims to summarize recommendations for all perioperative blood-conserving strategies in cardiac surgery

Global impact of the first coronavirus disease 2019 (COVID-19) pandemic wave on vascular services

This online structured survey has demonstrated the global impact of the COVID-19 pandemic on vascular services. The majority of centres have documented marked reductions in operating and services provided to vascular patients. In the months during recovery from the resource restrictions imposed during the pandemic peaks, there will be a significant vascular disease burden awaiting surgeons. One of the most affected specialtie