Validation of integrated pest management module against insect pests of pigeonpea, Cajanus cajan in Tarai region of Uttarakhand

Experiments on validation of integrated pest management (IPM) module against insect pest of pigeonpea in comparison with the Non-IPM (farmer’s practices) were conducted at N.E.B. Crop Research Centre, G. B. Pant University of Agriculture & Technology, Pantnagar during Kharif 2014 and 2015. Adopted IPM module contained Seed treatment with Trichoderma spp. @10g/kg of seed, Sole crop, Bird perches @ 50/ha, need based insecticides spray (Chlorantraniliprole 18.5SC @ 30 g a. i./ha; Neem soap@10g/lit; Acetamiprid 20SP @ 20 g a. i./ha). The results indicated that minimum population of pod borers (Helicoverpa armigera,Maruca vitrata and podfly) and sucking insects (aphids, jassids, pod bug) was reported in IPM plots and maximum population of insects was observed in Non-IPM plots. Percent insect control over non-IPM was 50.98 % for H. armigera, 44.69 % for M. vitrata and 19.17 % for Maruca webbing were recorded. While, for sucking pest complex, insect control over non-IPM was 51.59 %, 40.36 % and 36.17 % against jassids, aphids and tur pod bug, respectively. Similarly, minimum pod borer damage (6.48 and 7.71 %) was recorded in IPM plots as compared to maximum pod borer damage (8.37 and 8.22 %) in non-IPM plots, respectively during 2014 and 2015. Whereas, pooled grain yield for IPM plots was 1286.5 kg/ha for both seasons as against 888 kg/ha in non-IPM plots with 1:2.89 benefit cost ratio. Hence, It is apparent that studied IPM module was able to increase the yield of pigeonepea with lower cost of production as against non-IPM thus it would be benefiting the farmers

Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits

Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust

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Improving oil quality by altering levels of fatty acids through marker-assisted selection of ahfad2 alleles in peanut (Arachis hypogaea L.)

Peanut plays a key role to the livelihood of millions in the world especially in Arid and Semi-Arid regions. Peanut with high oleic acid content aids to increase shelf-life of peanut oil as well as food products and extends major health benefits to the consumers. In peanut, ahFAD2 gene controls quantity of two major fatty acids viz, oleic and linoleic acids. These two fatty acids together with palmitic acid constitute 90% fat composition in peanut and regulate the quality of peanut oil. Here, two ahfad2 alleles from SunOleic 95R were introgressed into ICGV 05141 using marker-assisted selection. Marker-assisted breeding effectively increased oleic acid and oleic to linoleic acid ratio in recombinant lines up to 44% and 30%, respectively as compared to ICGV 05141. In addition to improved oil quality, the recombinant lines also had superiority in pod yield together with desired pod/seed attributes. Realizing the health benefits and ever increasing demand in domestic and international market, the high oleic peanut recombinant lines will certainly boost the economical benefits to the Indian farmers in addition to ensuring availability of high oleic peanuts to the traders and industry