Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans.

Collaboration with: Université d’Orléans, 28000 Chartres, France, Université de Picardie Jules Verne, F-80037 Amiens, France De Montfort University Open access articleLinum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands

Laser ablation and static secondary ion mass spectrometry capabilities in the characterization of inorganic materials

Recently, mass spectrometry techniques such as laser ablation and static secondary ion mass spectrometry (LA-MS and s-SIMS, respectively) have been successfully applied to the characterization of inorganic compounds in solid state phase: s-SIMS is known as a surface analytical technique whereas LA-MS involves atoms in a greater thickness (bulk). In the case of s-SIMS, the direct ejection of ions from the surface upon primary ion sputtering for ion fluence down to 10(13) ions/cm(2), leads to a simple and direct diagnostic by comparing the spectra to databases. On the opposite, characterization of inorganic compounds by means of LA-MS is not immediate due to the most detected ions are issued from complex gas phase reactions. This feature can be successfully applied to investigate matter transfer processes occurring during pulsed-laser deposition (PLD) experiments. By the mean of a systematic and comparative study of LA-MS and s-SlMS spectra for binary (Cu-O) or ternary (Fe-Cr-O) oxide systems, we demonstrate that both techniques are complementary to each other in the field of material science. (C) 2002 Elsevier Science B.V. All rights reserved

Functionalization of gold nanoparticles by SPAAC reaction for PDT treatment of Dubreuilh’s melanoma.

International audienc

Synthesis and preliminary biological evaluations of PSMA-targeted NIR upconverting nanoparticles for optical/scintigraphic imaging of prostate cancer.

International audienc

Preliminary evaluations of PSMA-targeted NIR upconverting nanoparticles for dual scintigraphic/fluorescence-guided surgery in prostate cancer.

International audienc