Dosimetric planning of radioiodine therapy on the basis of pharmacokinetic modeling

The program complex of pharmacokinetic modeling and dosimetric planning of radioiodine therapy on the basis of clinical diagnostic data is developed. For 16 patients with the diagnosis «diffuse toxic goiter» (Graves' disease) individual kinetic parameters of transport of the thyroid radiopharmaceutical taken orally are identified and calculations of the absorbed doses in the thyroid, the stomach, the blood tissue, and the periodic-depletion bladder are performed. Three approaches to purpose of activity of radiopharmaceutical and feature of individual dosimetric planning of radioiodine therapy are considered and analysed

La vaisselle du sanctuaire de Mirebeau-sur-Bèze (Côte-d'Or) : faciès et évolution des corpus (IIIe s. av. J.-C. - IIe s. ap. J.-C.)

International audienc

Forskolin as a tool to study the β-adrenergic receptor-elicited, labeled protein secretion in rat lacrimal gland

AbstractIn rat lacrimal glands, Forskolin induces a dose-dependent [3H]protein release. This effect can be potentiated by papaverine. As for the other inducers whose effects on protein secretion are assumed to be cAMP-mediated, Forskolin secretion time course shows a latency. Isoproterenol decreases the Forskolin EC5- at least 60--times. On the other hand, Forskolin enhances the efficacy of isoproterenol without affecting its potency. As a whole, the data collected show that isoproterenol-induced [3H]protein secretion in rat lacrimal glands involved adenylate cyclase activation by coupling with β-adrenergic receptors

Les Elemens Des Sections Coniques, Démontrées Par Synthese : Ouvrage dans lequel on a renfermé le petit Traité des Sections Coniques de M. Delahire

Par M. M***Verfasser ermitteltPaginierfehler: Seite xix fälschlich als Seite xxi paginiertSupralibros der Naturforschenden Gesellschaft in Zürich Exemplar der ZB Züric

An Evaluation of Different Types of Peptone as Partial Substitutes for Animal-derived Serum in Vero Cell Culture

International audienceVero cells are one of the most frequently used cell types in virology. They can be used not only as a vehicle for the replication of viruses, but also as a model for investigating viral infectivity, cytopathology and vaccine production. There is increasing awareness of the need to limit the use of animal-derived components in cell culture media for a number of reasons, which include reducing the risk of contamination and decreasing costs related to the downstream processing of commercial products obtained via cell culture. The current study evaluates the use of protein hydrolysates (PHLs), also known as peptones, as partial substitutes for fetal bovine serum (FBS) in Vero cell culture. Eleven plant-based, two yeast-based, and three casein-based peptones were assessed, with different batches evaluated in the study. We tested the effects of three concentration ratios of FBS and peptone on Vero cell proliferation, four days after the initial cell seeding. Some of the tested peptones, when in combination with a minimal 1% level of FBS, supported cell proliferation rates equivalent to those achieved with 10% FBS. Collectively, our findings showed that plant-based peptones could represent promising options for the successful formulation of serum-reduced cell culture media for vaccine production. This is especially relevant in the context of the current COVID-19 pandemic, in view of the urgent need for SARS-CoV-2 virus production for certain types of vaccine. The current study contributes to the Three Rs principle of reduction, as well as addressing animal ethics concerns associated with FBS, by repurposing PHLs for use in cell culture

The sequential cleavage of membrane anchored pro-EGF requires a membrane serine protease other than kallikrein in rat kidney.

Epidermal growth factor (EGF) is present in kidney membranes as an integral type I precursor protein, enzymatically processed to release immunoreactive materials in urine or incubation medium. The aim of this work was the elucidation of both the anchor of the serine protease activity that processes pro-EGF, and the determination of the steps of the enzymatic processing. Quantification of EGF containing molecules by RIA following gel filtration analysis demonstrated that the membrane precursor is first shed from the kidney membrane principally into a 170-kDa soluble precursor. This entire ectodomain is further processed into a 70-kDa precursor and finally into the mature 5.9 kDa urinary EGF. These species correspond to the ones found in urines. Both shedding and maturation events are clearly realized by membrane anchored serine protease activity, which remains active in detergent. By use of wild-type and knockout mice urines, we found that tissue kallikrein (TK) was not involved in the regulation of this processing

Follow-up LOFAR observations of the τ Boötis exoplanetary system

International audienceContext. Observing the radio emission from exoplanets is among the most promising methods to detect their magnetic fields and a measurement of an exoplanetary magnetic field will help constrain the planet's interior structure, star-planet interactions, atmospheric escape and dynamics, and habitability. Recently, circularly polarized bursty and slow emission from the τ Boötis (τ Boo) exoplanetary system was tentatively detected using LOFAR (LOW-Frequency ARray) beamformed observations. If confirmed, this detection will be a major contribution to exoplanet science. However, follow-up observations are required to confirm this detection. Aims: Here, we present such follow-up observations of the τ Boo system using LOFAR. These observations cover 70% of the orbital period of τ Boo b including the orbital phases of the previous tentative detections. Methods: We used the BOREALIS pipeline to mitigate radio frequency interference and to search for bursty and slowly varying radio signals. BOREALIS was previously used to find the tentative radio signals from τ Boo. Results: Our new observations do not show any signs of bursty or slow emission from the τ Boötis exoplanetary system. Conclusions: The cause for our non-detection is currently degenerate. It is possible that the tentative radio signals were an unknown instrumental systematic or that we are observing variability in the planetary radio emission due to changes in its host star. More radio data (preferably multi-site) and ancillary observations (e.g. magnetic maps) are required to further investigate the potential radio emission from the τ Boötis exoplanetary system

Regulated cell surface pro-EGF ectodomain shedding is a zinc metalloprotease-dependent process.

International audienceEpidermal growth factor receptor (EGFR) ligands are synthesized as type I membrane protein precursors exposed at the cell surface. Shedding of the ectodomain of these proteins is the way cells regulate the equilibrium between cell-associated and diffusible forms of these growth factors. Whereas the regulated shedding of transforming growth factor-alpha, HB-EGF, and amphiregulin precursors have been clearly established, regulation of full-length pro-EGF shedding has not been clearly demonstrated. Here, using both wild-type and M2 mutant CHO-K1 as well as HeLa cell lines transiently transfected with epitope-tagged rat pro-EGF expression plasmid, we demonstrate that these cells synthesize EGF as a high molecular weight membrane-associated precursor glycoprotein expressed at the cell surface. All cell lines are able to release the entire ectodomain of pro-EGF in the extracellular medium following juxtamembrane cleavage of the precursor once it is present at the cell surface. More significantly we clearly established that CHO-M2 and HeLa cells only constitutively release low levels of pro-EGF. This shedding is a regulated phenomenon in wild-type CHO cells where it can be induced by different agents such as phorbol 12-myristate 13-acetate (PMA), pervanadate, and serum but not by calcium ionophores. Using specific inhibitors as well as protein kinase C (PKC) depletion, PMA stimulation was shown to be completely dependent on PKC activation whereas pervanadate and serum stimulation were not. Regulated ectodomain shedding involves the activity of a zinc metalloprotease as determined by inhibition with phenantrolin and TAPI-2 and by the results obtained with the CHO-M2 shedding defective mutant cell line. Comparison of the ability of CHO and HeLa cell lines to shed pro-EGF and pro-TNF-alpha upon stimulation greatly suggests that TACE (ADAM 17) may not be the ectoprotease involved in the secretion of pro-EGF ectodomain and that this protease, which remains to be identified, shows a restricted cellular expression pattern