30 research outputs found
Interactions of Graphene Oxide and Few-Layer Graphene with the BloodâBrain Barrier
We thank Dr. Michele Dipalo (Istituto Italiano di Tecnologia,
Genova, Italy) for help with Raman measurements. We also
thank Ilaria Dallorto, Rossana Ciancio, Diego Moruzzo, and
Arta Mehilli for administrative and technical help. The project
has received funding from the European Unionâs Horizon 2020
Research and Innovation Programme under Grant Agreement
No. 881603 Graphene Flagship Core3 (to F.B.), the Italian
Ministry of Foreign Affairs and International Cooperation
(Grant Agreement No. MAE00694702021-05-20 to F.B.), and
IRCCS Ospedale Policlinico San Martino, Genova, Italy
(Ricerca Corrente and â5x1000â to F.B and V.C.).Materials and methods and additional figures on materials
characterization, in vitro experiments, imaging and mass
spectrometry analysis. The Supporting Information is available
free of charge at https://pubs.acs.org/doi/10.1021/acs.nano-
lett.3c00377.
Materials and methods and additional figures on
materials characterization, in vitro experiments, imaging
and mass spectrometry analysis (PDF) https://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.3c00377/suppl_file/nl3c00377_si_001.pdf
Supplementary File P1: Full list of quantified proteins
(XLSX)
Supplementary File P2: Full sets of altered proteins
(XLSX)
Supplementary File P3: Full sets of altered proteins
(XLSX)Thanks to their biocompatibility and high cargo capability, graphene-based materials (GRMs) might represent an ideal brain delivery system. The capability of GRMs to reach the brain has mainly been investigated in vivo and has highlighted some controversy. Herein, we employed two in vitro BBB models of increasing complexity to investigate the bionano interactions with graphene oxide (GO) and few-layer graphene (FLG): a 2D murine Transwell model, followed by a 3D human multicellular assembloid, to mimic the complexity of the in vivo architecture and intercellular crosstalk. We developed specific methodologies to assess the translocation of GO and FLG in a label-free fashion and a platform applicable to any nanomaterial. Overall, our results show good biocompatibility of the two GRMs, which did not impact the integrity and functionality of the barrier. Sufficiently dispersed subpopulations of GO and FLG were actively uptaken by endothelial cells; however, the translocation was identified as a rare event.European Union's Horizon 2020 Research and Innovation Programme 881603Ministry of Foreign Affairs and International Cooperation (Italy) MAE00694702021-05-20IRCCS Ospedale Policlinico San Martino, Genova, Ital
Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation
The formation of the biomolecular corona represents a crucial factor in controlling the
biological interactions and trafficking of nanomaterials. In this context, the availability of key epitopes
exposed on the surface of the corona, and able to engage the biological machinery, is important to
define the biological fate of the material. While the full biomolecular corona composition can be
investigated by conventional bottom-up proteomics, the assessment of the spatial orientation of
proteins in the corona in a high-throughput fashion is still challenging. In this work, we show that
labeling corona proteins with isobaric tags in their native conditions and analyzing the MS/MS
spectra of tryptic peptides allow an easy and high-throughput assessment of the inner/outer
orientation of the corresponding proteins in the original corona. We put our results in the context of
what is currently known of the protein corona of graphene-based nanomaterials. Our conclusions are
in line with previous data and were confirmed by in silico calculations.European Commission
754446
881603European Union's Horizon 2020 under the Marie SklodowskaCurie Action-COFUND Athenea3i
75444
The potential of graphene coatings as neural interfaces
Recent advances in nanotechnology design and fabrication have shaped the landscape for the
development of ideal cell interfaces based on biomaterials. A holistic evaluation of the requirements for a
cell interface is a highly complex task. Biocompatibility is a crucial requirement which is affected by the
interfaceâs properties, including elemental composition, morphology, and surface chemistry. This review
explores the current state-of-the-art on graphene coatings produced by chemical vapor deposition (CVD)
and applied as neural interfaces, detailing the key properties required to design an interface capable of
physiologically interacting with neural cells. The interfaces are classified into substrates and scaffolds to
differentiate the planar and three-dimensional environments where the cells can adhere and proliferate.
The role of specific features such as mechanical properties, porosity and wettability are investigated. We
further report on the specific brain-interface applications where CVD graphene paved the way to
revolutionary advances in biomedicine. Future studies on the long-term effects of graphene-based
materials in vivo will unlock even more potentially disruptive neuro-applications.Project
2DM4EH with reference DRI/India/0664/2020, funded by
FCTâScience and Technology FoundationMICIN/AEI/10.13039/501100011033European
Social Found (FSE) El FSE invierte en tu futuro for RamĂłn y
Cajal research contract (RYC-2019â027692-I
Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses
Extracellular pH impacts on neuronal activity, which is in turn an important determinant of extracellular H+ concentration. The aim of this study is to describe the spatio-temporal dynamics of extracellular pH at synaptic sites during neuronal hyperexcitability. To address this issue we created ex.E2GFP, a membrane-targeted extracellular ratiometric pH indicator exquisitely sensitive to acidic shifts. By monitoring ex.E2GFP fluorescence in real time in primary cortical neurons we were able to quantify pH fluctuations during network hyperexcitability induced by convulsant drugs or high frequency electrical stimulation. Sustained hyperactivity caused a pH decrease that was reversible upon silencing of neuronal activity and localized to active synapses. This acidic shift was not attributable to the outflow of synaptic vesicle protons into the cleft nor to the activity of membrane-exposed H+-vATPase, but rather to the activity of the Na+/H+-exchanger. Our data demonstrate that extracellular synaptic pH shifts take place during epileptic-like activity of neural cultures, underlying the strict links existing between synaptic activity and synaptic pH. This evidence may contribute to the understanding of the physio-pathological mechanisms associated with hyperexcitability in the epileptic brain
Graphene nanoplatelets render poly(3-hydroxybutyrate) a suitable scaffold to promote neuronal network development
The use of composite biomaterials as innovative bio-friendly neuronal interfaces has been poorly developed so far. Smart strategies to target neuro-pathologies are currently exploiting the mixed and complementary characteristics of composite materials to better design future neural interfaces. Here we present a polymer-based scaffold that has been rendered suitable for primary neurons by embedding graphene nanoplatelets (GnP). In particular, the growth, network formation, and functionality of primary neurons on poly(3-hydroxybutyrate) [P(3HB)] polymer supports functionalized with various concentrations of GnP were explored. After growing primary cortical neurons onto the supports for 14 days, all specimens were found to be biocompatible, revealing physiological growth and maturation of the neuronal network. When network functionality was investigated by whole patch-clamp measurements, pure P(3HB) led to changes in the action potential waveform and reduction in firing frequency, resulting in decreased neuronal excitability. However, the addition of GnP to the polymer matrix restored the electrophysiological parameters to physiological values. Interestingly, a low concentration of graphene was able to promote firing activity at a low level of injected current. The results indicate that the P(3HB)/GnP composites show great potential for electrical interfacing with primary neurons to eventually target central nervous system disorders
Graphene Oxide Upregulates the Homeostatic Functions of Primary Astrocytes and Modulates Astrocyte-to-Neuron Communication
Graphene-based materials are the focus of intense research efforts to devise novel theranostic strategies for targeting the central nervous system. In this work, we have investigated the consequences of long-term exposure of primary rat astrocytes to pristine graphene (GR) and graphene oxide (GO) flakes. We demonstrate that GR/GO interfere with a variety of intracellular processes as a result of their internalization through the endolysosomal pathway. Graphene-exposed astrocytes acquire a more differentiated morphological phenotype associated with extensive cytoskeletal rearrangements. Profound functional alterations are induced by GO internalization, including the upregulation of inward-rectifying K+ channels and of Na+-dependent glutamate uptake, which are linked to the astrocyte capacity to control the extracellular homeostasis. Interestingly, GO-pretreated astrocytes promote the functional maturation of co-cultured primary neurons by inducing an increase in intrinsic excitability and in the density of GABAergic synapses. The results indicate that graphene nanomaterials profoundly affect astrocyte physiology in vitro with consequences for neuronal network activity. This work supports the view that GO-based materials could be of great interest to address pathologies of the central nervous system associated with astrocyte dysfunctions
An Increase in Membrane Cholesterol by Graphene Oxide Disrupts Calcium Homeostasis in Primary Astrocytes
The use of graphene nanomaterials (GNMs) for biomedical applications targeted to the central nervous system is exponentially increasing, although precise information on their effects on brain cells is lacking. In this work, the molecular changes induced in cortical astrocytes by few-layer graphene (FLG) and graphene oxide (GO) flakes are addressed. The results show that exposure to FLG/GO does not affect cell viability or proliferation. However, proteomic and lipidomic analyses unveil alterations in several cellular processes, including intracellular Ca2+ ([Ca2+ ]i ) homeostasis and cholesterol metabolism, which are particularly intense in cells exposed to GO. Indeed, GO exposure impairs spontaneous and evoked astrocyte [Ca2+ ]i signals and induces a marked increase in membrane cholesterol levels. Importantly, cholesterol depletion fully rescues [Ca2+ ]i dynamics in GO-treated cells, indicating a causal relationship between these GO-mediated effects. The results indicate that exposure to GNMs alters intracellular signaling in astrocytes and may impact astrocyte-neuron interactions
Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers
Cellular barriers, such as the skin, the lung epithelium or the intestinal epithelium, constitute one of the first obstacles facing nanomedicines or other nanoparticles entering organisms. It is thus important to assess the capacity of nanoparticles to enter and transport across such barriers. In this work, Caco-2 intestinal epithelial cells were used as a well-established model for the intestinal barrier, and the uptake, trafficking and translocation of model silica nanoparticles of different sizes were investigated using a combination of imaging, flow cytometry and transport studies. Compared to typical observations in standard cell lines commonly used for in vitro studies, silica nanoparticle uptake into well-developed Caco-2 cellular barriers was found to be very low. Instead, nanoparticle association to the apical outer membrane was substantial and these particles could easily be misinterpreted as internalised in the absence of imaging. Passage of nanoparticles through the barrier was very limited, suggesting that the low amount of internalised nanoparticles was due to reduced uptake into cells, rather than a considerable transport through them