Cultivation of Thyrsopteris elegans

Thyrsopteris elegans Kunze is a monospecific genus of fern endemic to the Juan Fernandez Islands, off the coast of Chile. These islands have large numbers of alien invasive species and the native flora, including T elegans which already has a very restricted distribution, is consequently under considerable threat. One of the largest examples of this species in cultivation in Britain grows at the Royal Botanic Garden Edinburgh and this paper describes its propagationand cultivation

Nymphs of the Stonefly (Plecoptera) Genus Taeniopteryx of North America

Nymphs of the 9 Nearctic Taeniopteryx species were reared and studied, 1976-78. Two morphologically allied groupings, the Taeniopteryx burksi-maura, and T. litalonicera- starki complexes corresponded with adult complexes. A key separating 7 species, based primarily upon pigment patterns and abdominal setal arrangements, was constructed. Taeniopteryx lita and T. starki were indistinguishable; T. burksi was separated from T. maurawhen no developing femoral spur was present. This study was based upon 839 nymphs. Mouthparts were not species-diagnostic. Detailed habitus illustrations were made for 6 species. Egg SEM study revealed that 3 species were 1.2-1.4 mm diameter, with a highly sculptured chorion, generally resembling a Maclura fruit; micropyle were scattered. Taeniopteryx lita, lonicera, starki and ugola nymphs were described for the first time

Exploring Literacy Coaching as a Form of Staff Development

Following a 2011 audit a school district in the south central United States clarified the role of the literacy coach. However, there were still differences among the literacy coaches as to how they were performing their duties. As a result, the purpose of this study was to gain an understanding of the role of the literacy coach in the participating school district. The theoretical foundation of this study addressed adult learning and was based upon Kegan\u27s constructive development theory and Knowles\u27s theory of androgogy. A case study design was used to explore how 5 literacy coaches implemented literacy staff development with over 100 elementary school teachers and what aspects of literacy instruction were focused upon. Data were collected through interviews and daily coaching logs kept by the coaches. Each interview was recorded, transcribed, and analyzed through a system of coding based on repeated readings, from which themes, concepts, similarities, and differences became apparent. Similarities and differences were highlighted, and tables were created to track them. Coaching logs were collected and analyzed in the same manner. Two themes emerged from analysis of the data: identifying themselves as staff developers and needing more teacher collaboration. Based upon these themes, professional development training sessions were developed to strengthen the professional development already in place, and the creation of professional learning communities was recommended. Participation in these activities will strengthen individual literacy teacher\u27s professional knowledge regarding the teaching of literacy. As a result literacy teachers\u27 practices will improve, and in turn, positive social change will occur when the children they teach become more literate, increase their learning, and stay in school

Identification of Epstein-Barr virus replication proteins in Burkitt’s lymphoma cells

The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications

Effect of short term diet restriction on gene expression in the bovine hypothalamus using next generation RNA sequencing technology

peer-reviewedThis work was funded through Teagasc Walsh Fellowship to Daragh Matthews (Project Number: RMIS 5756).Background Negative energy balance (NEB) is an imbalance between energy intake and energy requirements for lactation and body maintenance affecting high-yielding dairy cows and is of considerable economic importance due to its negative impact on fertility and health in dairy herds. It is anticipated that the cow hypothalamus experiences extensive biochemical changes during the early post partum period in an effort to re-establish metabolic homeostasis. However, there is variation in the tolerance to NEB between individual cows. In order to understand the genomic regulation of ovulation in hypothalamic tissue during NEB, mRNA transcriptional patterns between tolerant and sensitive animals were examined. A short term dietary restriction heifer model was developed which induced abrupt onset of anoestrus in some animals (Restricted Anovulatory; RA) while others maintained oestrous cyclicity (Restricted Ovulatory; RO). A third control group (C) received a higher level of normal feeding. Results A total of 15,295 genes were expressed in hypothalamic tissue. Between RA and C groups 137 genes were differentially expressed, whereas between RO and C, 32 genes were differentially expressed. Differentially expressed genes were involved in the immune response and cellular motility in RA and RO groups, respectively, compared to C group. The largest difference between groups was observed in the comparison between RA and RO heifers, with 1094 genes shown to be significantly differentially expressed (SDE). Pathway analysis showed that these SDE genes were associated with 6 canonical pathways (P < 0.01), of which neuroactive ligand-receptor interaction was the most significant. Within the comparisons the main over-represented pathway functions were immune response including neuroprotection (CXCL10, Q1KLR3, IFIH1, IL1 and IL8; RA v C and RA v RO); energy homeostasis (AgRP and NPY; RA v RO); cell motility (CADH1, DSP and TSP4; RO v C) and prevention of GnRH release (NTSR1 IL1α, IL1β, NPY and PACA; RA v RO). Conclusions This information will assist in understanding the genomic factors regulating the influence of diet restriction on fertility and may assist in optimising nutritional and management systems for the improvement in reproductive performance.Teagasc Walsh Fellowship Programm

A deep search for planets in the inner 15 au around Vega

We present the results of a deep high-contrast imaging search for planets around Vega. Vega is an ideal target for high-contrast imaging because it is bright, nearby, and young with a face-on two-belt debris disk which may be shaped by unseen planets. We obtained $J-$ and $H-$band data on Vega with the coronagraphic integral-field spectrograph Project 1640 (P1640) at Palomar Observatory. Two nights of data were obtained in 2016, in poor seeing conditions, and two additional nights in more favorable conditions in 2017. In total, we obtained 5.5 hours of integration time on Vega in moderate to good seeing conditions (<1.5"). We did not detect any low mass companions in this system. Our data present the most sensitive contrast limits around Vega at very small separations (2-15 au) thus far, allowing us to place new constraints on the companions which may be sculpting the Vega system. In addition to new constraints, as the deepest data obtained with P1640, these observations form the final legacy of the now decommissioned instrument.Comment: Accepted for publication in A

Resolved Millimeter Observations of the HR 8799 Debris Disk

We present 1.3 millimeter observations of the debris disk surrounding the HR 8799 multi-planet system from the Submillimeter Array to complement archival ALMA observations that spatially filtered away the bulk of the emission. The image morphology at $3.8$ arcsecond (150 AU) resolution indicates an optically thin circumstellar belt, which we associate with a population of dust-producing planetesimals within the debris disk. The interferometric visibilities are fit well by an axisymmetric radial power-law model characterized by a broad width, $\Delta R/R\gtrsim 1$. The belt inclination and orientation parameters are consistent with the planet orbital parameters within the mutual uncertainties. The models constrain the radial location of the inner edge of the belt to $R_\text{in}= 104_{-12}^{+8}$ AU. In a simple scenario where the chaotic zone of the outermost planet b truncates the planetesimal distribution, this inner edge location translates into a constraint on the planet~b mass of $M_\text{pl} = 5.8_{-3.1}^{+7.9}$ M$_{\rm Jup}$. This mass estimate is consistent with infrared observations of the planet luminosity and standard hot-start evolutionary models, with the uncertainties allowing for a range of initial conditions. We also present new 9 millimeter observations of the debris disk from the Very Large Array and determine a millimeter spectral index of $2.41\pm0.17$. This value is typical of debris disks and indicates a power-law index of the grain size distribution $q=3.27\pm0.10$, close to predictions for a classical collisional cascade.Comment: 18 pages, 7 figures, accepted by Ap