Orientation Optimization Based on Topological Derivatives in Cooperation with Multi-Material Topology Optimization Based on Extended Level Set Method

This paper provides an orientation angle optimization method for the design of fiber-reinforced composite materials using topology optimization. The orientation angle optimization is based on a topological derivative, which measures the sensitivity of an objective function with respect to a topological change of anisotropic materials. The sensitivity is incorporated into a new gradient-based optimization algorithm. This method allows us to avoid local optima and seek a global optimal solution. We provide some numerical examples and verify the effectiveness of the proposed method.Comment: 24 pages, 16 figure

Seismic refraction and wide-angle reflection exploration by JARE-43 on Mizuho Plateau, East Antarctica

The 43rd Japanese Antarctic Research Expedition (JARE-43) carried out seismic exploration experiments on Mizuho Plateau,East Antarctica,in the austral summer season of ,2001-2002. The exploration was composed of seven large explosions and 161seismic stations distributed along the 151 km-long seismic line.The first arrival time data are analyzed by a refraction method.It is found that the ice sheet is composed of two layers:the upper layer with P wave velocity of 2.7-2.9 km/s has thickness of 35-45 m,and the lower layer with P wave velocity of 3.7-3.9 km/s continues to the bedrock.Lateral velocity variation in the upper-most crust is revealed:P wave velocity for the upper-most crust in the southern and central parts is 6.1-6.2 km/s and that in the northern part is5.9 km/s. This result implies that the geological boundary observed along the coast in the Lu tzow-Holm Bay and Prince Olav Coast regions possibly continues to the inland area.The S wave velocity is also obtained to be roughly 3.5km/s for the whole upper-most crust.Travel time analysis of two distinct reflection phases shows two horizontal reflecting planes located at 19 and 40km depth; the latter corresponds to the Moho discontinuity

Nongyrotropic electron velocity distribution functions near the lunar surface

We have analyzed nongyrotropic electron velocity distribution functions (VDFs) obtained near the lunar surface. Electron VDFs, measured at ∼10–100 km altitude by Kaguya in both the solar wind and the Earth's magnetosphere, exhibit nongyrotropic empty regions associated with the ‘gyroloss’ effect; i.e., electron absorption by the lunar surface combined with electron gyromotion. Particle-trace calculations allow us to derive theoretical forbidden regions in the electron VDFs, thereby taking into account the modifications due to nonuniform magnetic fields caused by diamagnetic-current systems, lunar-surface charging, and electric fields perpendicular to the magnetic field. Comparison between the observed empty regions with the theoretically derived forbidden regions suggests that various components modify the characteristics of the nongyrotropic electron VDFs depending on the ambient-plasma conditions. On the lunar nightside in the magnetotail lobes, negative surface potentials slightly reduce the size of the forbidden regions, but there are no distinct effects of either the diamagnetic current or perpendicular electric fields. On the dayside in the solar wind, the observations suggest the presence of either the diamagnetic-current or solar wind convection electric field effects, or both. In the terrestrial plasma sheet, all three mechanisms can substantially modify the characteristics of the forbidden regions. The observations imply the presence of a local electric field of at least 5 mV/m although the mechanism responsible for production of such a strong electric field is unknown. Analysis of nongyrotropic VDFs associated with the gyroloss effect near solid surfaces can promote a better understanding of the near-surface plasma environment and of plasma–solid-surface interactions

シボウ サイボウ ノ カガク

Adipose tissue secretes a variety of bioactive molecules (adipocytokines) that directlycontribute to the development of life-style related diseases, such as diabetes, hypertension,and cardiovascular diseases. TNF-α is a well - known inhibitor of insulin signaling and PAI(plasminogen activator inhibitor) - 1 contributes to thrombus formation. Adiponectin has anaction both insulin sensitivity and anti - atherogenic action. Interestingly, low plasmaadiponectin concentrations were observed in patients with obesity, coronary artery disease(CAD), and type 2 diabetes. Adiponectin from the plasma adhered to the injured arteryand suppressed the endothelial expression of adhesion molecules, the proliferation ofvascular smooth muscle cells, and the transformation of macrophage to foam cells. Thesefindings support that accumulation of fat produces not only insulin resistance but alsoatherosclrosis in obese subjects

Performance Test of Infrasound Sensor in Low-temperature Environment ─ Potential for Application in Antarctic Observation ─

For infrasound monitoring in Antarctica, there is a need for infrasound sensors with low power consumption and high resistance to low-temperature environments. A new-type infrasound sensor (TYPE7744N/5002A) manufactured by ACO Co., Ltd. (Japan) with the cooperation of the Earthquake Research Institute, the University of Tokyo, achieves less than half the power consumption of existing models. To evaluate the applicability of the new sensor to Antarctic observation, we conducted a low-temperature (－30℃) test for four types of sensors, including the new one. We compared the results to those from a room temperature (21℃) test and examined changes in amplitude-phase characteristics based on a reference sensor (Model60Vx2, Chaparral Physics), proven in use in polar regions. There were no problems in the operation of the new sensor during the 30 days of the test. Spectral power ratio to the reference sensor changed up to 19% compared to the room temperature test, suggesting that the sensitivity fluctuates with temperature. Phase characteristics were not significantly affected by low temperatures. Future trials are desired to evaluate the long-term stability of the new sensor, e.g., by conducting experimental overwintering observations at Syowa Station

The novel heart-specific RING finger protein 207 is involved in energy metabolism in cardiomyocytes

A failing heart shows severe energy insufficiency, and it is presumed that this energy shortage plays a critical role in the development of cardiac dysfunction. However, little is known about the mechanisms that cause energy metabolic alterations in the failing heart. Here, we show that the novel RING-finger protein 207 (RNF207), which is specifically expressed in the heart, plays a role in cardiac energy metabolism. Depletion of RNF207 in neonatal rat cardiomyocytes (NRCs) leads to a reduced cellular concentration of adenosine triphosphate (ATP) and mitochondrial dysfunction. Consistent with this result, we observed here that the expression of RNF207 was significantly reduced in mice with common cardiac diseases including heart failure. Intriguingly, proteomic approaches revealed that RNF207 interacts with the voltage-dependent anion channel (VDAC), which is considered to be a key regulator of mitochondria function, as an RNF207-interacting protein. Our findings indicate that RNF207 is involved in ATP production by cardiomyocytes, suggesting that RNF207 plays an important role in the development of heart failure

MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells

Background: Esophageal squamous cell carcinoma (ESCC) is often diagnosed at later stages until they are incurable. MicroRNA (miR) is a small, non-coding RNA that negatively regulates gene expression mainly via translational repression. Accumulating evidence indicates that deregulation of miR is associated with human malignancies including ESCC. The aim of this study was to identify miR that could be specifically expressed and exert distinct biological actions in ESCC. Methods: Total RNA was extracted from ESCC cell lines, OE21 and TE10, and a non-malignant human esophageal squamous cell line, Het-1A, and subjected to microarray analysis. Expression levels of miR that showed significant differences between the 2 ESCC and Het-1A cells based on the comprehensive analysis were analyzed by the quantitative reverse transcriptase (RT)-PCR method. Then, functional analyses, including cellular proliferation, apoptosis and Matrigel invasion and the wound healing assay, for the specific miR were conducted. Using ESCC tumor samples and paired surrounding non-cancerous tissue obtained endoscopically, the association with histopathological differentiation was examined with quantitative RT-PCR. Results: Based on the miR microarray analysis, there were 14 miRs that showed significant differences (more than 2-fold) in expression between the 2 ESCC cells and non-malignant Het-1A. Among the significantly altered miRs, miR-205 expression levels were exclusively higher in 5 ESCC cell lines examined than any other types of malignant cell lines and Het-1A. Thus, miR-205 could be a specific miR in ESCC. Modulation of miR-205 expression by transfection with its precursor or anti-miR-205 inhibitor did not affect ESCC cell proliferation and apoptosis, but miR-205 was found to be involved in cell invasion and migration. Western blot revealed that knockdown of miR-205 expression in ESCC cells substantially enhanced expression of zinc finger E-box binding homeobox 2, accompanied by reduction of E-cadherin, a regulator of epithelial mesenchymal transition. The miR-205 expression levels were not associated with histological differentiation of human ESCC. Conclusions: These results imply that miR-205 is an ESCC-specific miR that exerts tumor-suppressive activities with EMT inhibition by targeting ZEB2.Kayoko Matsushima... Gregory J Goodall... et al

New findings on the fungal species Tricholoma matsutake from Ukraine, and revision of its taxonomy and biogeography based on multilocus phylogenetic analyses

Publisher Copyright: © 2022 Elsevier B.V.. All rights reserved.Matsutake mushrooms are among the best-known edible wild mushroom taxa worldwide. The representative Tricholoma matsutake is from East Asia and the northern and central regions of Europe. Here, we report the existence of T. matsutake under fir trees in Eastern Europe (i.e., Ukraine), as confirmed by phylogenetic analysis of nine loci on the nuclear and mitochondrial genomes. All specimens from Japan, Bhutan, China, North Korea, South Korea, Sweden, Finland, and Ukraine formed a T. matsutake clade according to the phylogeny of the internal transcribed spacer region. The European population of T. matsutake was clustered based on the β2 tubulin gene, with a moderate bootstrap value. In contrast, based on analyses of three loci, i.e., rpb2, tef1, and the β2 tubulin gene, T. matsutake specimens sampled from Bhutan and China belonged to a clade independent of the other specimens of this species, implying a genetically isolated population. As biologically available type specimens of T. matsutake have not been designated since its description as a new species from Japan in 1925, we established an epitype of this fungus, sampled in a Pinus densiflora forest in Nagano, Japan.Peer reviewe