Validação de 3 Equipamentos de TDR (Reflectometria no Domínio do Tempo) para a Medida da Umidade de Solos.

bitstream/CNPDIA/10455/1/CT61_2004.pd

Beta-glucan reflects liver injury after preservation and transplantation in dogs.

Graft failure and extrahepatic organ complications, which frequently develop after transplantation, may be related to inflammatory mediators stimulated by endotoxin (ET). The role of endotoxemia after liver transplantation is controversial and may depend upon differences in the ET assay method used in the various contradicting studies. While the standard Limulus amebocyte lysate (LAL) is reactive for ET and beta-glucan, a novel turbidimetric assay method enables separate determinations of ET and beta-glucan. Beagle dogs undergoing orthotopic liver transplantation were divided into two groups. In Group I (n = 6) the grafts were transplanted immediately and in Group II (n = 6) grafts were preserved for 48 h in University of Wisconsin (UW) solution. Animals received cyclosporine immunosuppression and were followed for 14 days. Daily measurements of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) were performed. Samples for ET and beta-glucan measurement were collected serially and processed using the turbidimetric assay method. While no graft failure was seen in Group I, three of six Group II animals died from graft failure within 1 day after transplantation. Preservation and reperfusion injury was much more severe in the Group II grafts than in Group I grafts. While endotoxemia could not be detected, postoperative beta-glucan levels (undetectable pretransplant) were seen in both groups. Beta-glucan levels were much higher in Group II grafts than in Group I grafts, and correlated with the severity of liver damage. In conclusion, this study shows that beta-glucan, instead of ET, appears during the early posttransplant period. We believe that posttransplant elevation of beta-glucan is related to liver damage, especially endothelial damage by preservation and reperfusion

Bulbos úmidos a partir da irrigação por gotejamento subsuperficial com água de abastecimento e efluente de esgoto tratado

The use of treated sewage effluent (TSE) combined with the subsurface drip irrigation (SDI) method in agriculture can decrease the costs of agricultural production, in attempts to fertigate crops more efficiently. In this study it was compared the dimensions of the wet bulb formed by the application of TSE and municipal water supply (MWS) in an Oxisoil. We have evaluated the effect of water quality and discharge between drippers used in sugarcane crop. Three trenches were opened and 21 three-rod TDR probes were setup in a mesh and a dripper was buried at 0.30 m, for each constant discharge of 1.0 L h-1and 1.6 L h-1. Comparing results from different wetted soil profiles it was observed that the vertical and horizontal dimensions of the wet bulb are similar for both MWS and TSE, being peculiars according to the discharges used and volume applied. Regardless the water quality, an increase of 60% in discharge decreased the deepest infiltration.352242253O uso de efluente de esgoto tratado (EET), combinado com o método de irrigação por gotejamento subsuperfical (IGS) na agricultura, pode reduzir os custos de produção agrícola, fertirrigando de maneira eficiente as culturas. Neste artigo, compararam-se as dimensões do bulbo úmido formado pela aplicação de EET e água de abastecimento municipal (AAM), em um Latossolo Vermelho distroférrico. Avaliou-se o efeito da qualidade da água e da vazão entre gotejadores utilizados na cultura da cana-de-açúcar. Três trincheiras foram abertas e 21 sondas de TDR com três hastes foram instaladas em malha, e um gotejador foi enterrado a 0,30 m, para cada vazão constante de 1,0 Lh-1 e 1,6 Lh-1. Comparando os resultados de diferentes perfis de umidade do solo, sugere-se que as dimensões vertical e horizontal do bulbo úmido sejam similares para a AAM e para o EET, sendo peculiares de acordo com as vazões utilizadas e o volume aplicado. Independentemente da qualidade da água, o aumento de 60% na vazão reduziu a infiltração em profundidade

Distribuição do sistema radicular da cana-de-açúcar irrigada com efluente de esgoto doméstico por gotejamento subsuperficial

Irrigation with domestic sewage effluent (DSE) has been recommended by subsurface dripping, as it can obtain a high rate of irrigation efficiency and faster use of salts in comparison with other irrigation methods. The study aimed at evaluating the area, the length and the effective depth of the root system of sugarcane irrigated with DSE by subsurface drip system and with different irrigation rates at depths of 0.00-0.20, 0.20-0.40, 0.40-0.60 and 0.60-0.80m. The experiment was carried out in the municipality of Piracicaba, in the state of São Paulo (SP), Brazil, in a sugarcane area irrigated with DSE in a completely randomized blocks set up in furrows, with three replications and four treatments, which are: one area without irrigation (AWI) and three irrigated areas meeting 50% (T50%), 100% (T100%) and 200% (T200%) of the crop's water need between each round of irrigation. T100% and T200% provided smaller areas and lengths of roots in the two deepest layers, as compared to AWI and T50%, which stimulated the development of deeper roots due to the water stress. TWI, T100% and T200% presented 80% of the roots up to a depth of 0.40m and T50% treatment presented 76.43% of roots total.A irrigação com efluente de esgoto doméstico (EED) tem sido recomendada para aplicação por gotejamento subsuperficial, podendo-se obter elevados índices de eficiência de irrigação e mais rápido aproveitamento dos sais em comparação com outros métodos de irrigação. O trabalho objetivou avaliar a área de raiz, o comprimento e a profundidade efetiva do sistema radicular da cana-de-açúcar irrigada com EED aplicado por gotejamento subsuperficial e com diferentes lâminas de irrigação, nas camadas de solo de 0,00-0,20; 0,20-0,40; 0,40-0,60 e 0,60-0,80 m. O experimento foi realizado em Piracicaba-SP, numa área de cana-de-açúcar irrigada com EED, em um delineamento de blocos casualizados dispostos no esquema de faixas, com três repetições e quatro tratamentos, sendo estes: uma área sem irrigação (TSI) e três áreas irrigadas, atendendo a 50% (T50%), 100% (T100%) e 200% (T200%) da necessidade hídrica da cultura entre cada turno de rega. O T100% e o T200% proporcionaram menores áreas e comprimento de raízes nas duas camadas mais profundas, quando comparados ao TSI e ao T50%, que devido ao estresse hídrico, proporcionado à cultura, estimularam o desenvolvimento de raízes mais profundas. O TSI, T100% e T200% apresentaram 80% das raízes até 0,40 m e, nessa mesma profundidade, o tratamento T50% apresentou 76,43% do total de raízes.64765

Uniformity of water distribution in a central pivot with the use of the Time Domain Reflectometry technique on surface and soil

The evaluation of the uniformity of water distribution in an irrigation system is done above the surface of the soil. The main objective of this work was to evaluate the uniformity of water distribution in a central pivot under field conditions, through the use of the CUD and CUC coefficients. On the surface, the uniformity was obtained with the use of the ABNT methodology, therefore placing two collector lines displaced three degrees from the initial pivot position. In order to obtain the uniformity in the soil, the water content was determined with the TDR (Time Domain Reflectometry), which is a simple and fast technique. The results demonstrated that uniformity in the soil was greater than on the surface and above the recommended range. The water arrangement in the soil promotes that condition. Therefore in the economic design of irrigation systems, the uniformity coefficients in the soil should be considered.A avaliação da uniformidade de distribuição de água de um sistema de irrigação é feita acima da superfície do solo. Tendo em vista a importância da uniformidade no interior do solo quando submetido a uma irrigação, desenvolveu-se o presente trabalho com o objetivo de se avaliar a uniformidade de distribuição de água, tanto acima como no interior do solo, através dos coeficientes CUD e CUC, com um pivô central operando em condições de campo. Acima da superfície, a uniformidade foi obtida seguindo-se a metodologia da Associação Brasileira de Normas Técnicas (ABNT) e se alocando duas linhas de coletores com abertura de 3º, a partir do ponto inicial do pivô. Propôs-se, para obtenção da uniformidade no interior do solo, a utilização da umidade determinada pelo TDR (Reflectometria no Domínio do Tempo) visto que é uma técnica simples e produz resultados imediatos. Os resultados encontrados demonstraram que a uniformidade no interior do solo foi maior que na superfície, ficando acima da faixa recomendada, mostrando que a redistribuição da água no interior do solo promove maior uniformidade de umidade no perfil do solo que a encontrada na lâmina aplicada; portanto, no dimensionamento econômico de sistemas de irrigação, os coeficientes de uniformidade obtidos no interior do solo devem ser considerados.18719

Higgs-Boson Decay to Four Fermions Including a Single Top Quark Below ttˉt \bar t Threshold

The rare decay modes Higgs $\rightarrow$ four light fermions, and Higgs $\rightarrow$ single top-quark + three light fermions for $m_t<M_H<2m_t$, are presented, and phenomenologically interpreted. The angular correlation between fermion planes is presented as a test of the spin and intrinsic parity of the Higgs particle. In Higgs decay to single top, two tree-level graphs contribute in the standard model (SM); one couples the Higgs to $W^+W^-(\sim gM_W)$, and one to t\bar t(\sim g_{top\;yukawa}=m_t/246\GeV). The large Yukawa coupling for m_t>100\GeV makes the second amplitude competitive or dominant for most $M_H,m_t$ values. Thus the Higgs decay rate to single top directly probes the SM universal mechanism generating both gauge boson and fermion masses, and offers a means to infer the Higgs-$t \bar t$ Yukawa coupling when $H\rightarrow t \bar t$ is kinematically disallowed. We find that the modes $pp\rightarrow Xt\bar t(H\rightarrow t\bar b W^{(*)})$ at the SSC, and $e^+ e^-\rightarrow Z\,or\,\nu\bar{\nu} + (H\rightarrow t\bar b W^{(*)})$ at future high energy, high luminosity colliders, may be measureable if $2m_t$ is not too far above $M_H$. We classify non-standard Higgses as gaugeo-phobic, fermio-phobic or fermio-philic, and discuss the Higgs$\rightarrow$ single top rates for these classes.Comment: 30 pages, 6 figures (figures available upon request); VAND-TH-93/

Jack of All Trades, Master of All: A Positive Association between Habitat Niche Breadth and Foraging Performance in Pit-Building Antlion Larvae

Species utilizing a wide range of resources are intuitively expected to be less efficient in exploiting each resource type compared to species which have developed an optimal phenotype for utilizing only one or a few resources. We report here the results of an empirical study whose aim was to test for a negative association between habitat niche breadth and foraging performance. As a model system to address this question, we used two highly abundant species of pit-building antlions varying in their habitat niche breadth: the habitat generalist Myrmeleon hyalinus, which inhabits a variety of soil types but occurs mainly in sandy soils, and the habitat specialist Cueta lineosa, which is restricted to light soils such as loess. Both species were able to discriminate between the two soils, with each showing a distinct and higher preference to the soil type providing higher prey capture success and characterizing its primary habitat-of-origin. As expected, only small differences in the foraging performances of the habitat generalist were evident between the two soils, while the performance of the habitat specialist was markedly reduced in the alternative sandy soil. Remarkably, in both soil types, the habitat generalist constructed pits and responded to prey faster than the habitat specialist, at least under the temperature range of this study. Furthermore, prey capture success of the habitat generalist was higher than that of the habitat specialist irrespective of the soil type or prey ant species encountered, implying a positive association between habitat niche-breadth and foraging performance. Alternatively, C. lineosa specialization to light soils does not necessarily confer upon its superiority in utilizing such habitats. We thus suggest that habitat specialization in C. lineosa is either an evolutionary dead-end, or, more likely, that this species' superiority in light soils can only be evident when considering additional niche axes

Melatonin inhibira lipidnu peroksidaciju u jetri štakora uzrokovanu benzenom

We studied the antioxidative role of melatonin against benzene toxicity in rat liver. The inhibition of mitochondrial and microsomal lipid peroxidation differed between 24-hour (single-dose), 15-day, and 30-day treatments. Inhibition of mitochondrial lipid peroxidation was the highest after the single dose of melatonin, whereas highest microsomal inhibition was recorded after 30 days of melatonin treatment. No signifi cant difference was recorded between 15-day and 30-day treatments. Cytochrome P4502E1 (CYP4502E1) activity declined after the single-dose and 15-day melatonin treatment in the benzenetreated group, but it rose again, though not signifi cantly after 30 days of treatment. Liver histopathology generally supported these fi ndings. Phenol concentration in the urine samples declined in melatonin and benzene-treated rats. Our results show that melatonin affects CYP4502E1, which is responsible for benzene metabolism. Inhibition of its metabolism correlated with lower lipid peroxidation. In conclusion, melatonin was found to be protective against lipid peroxidation induced by benzene.Istražena je antioksidacijska uloga melatonina u zaštiti protiv toksičnoga djelovanja benzena u jetri štakora. Utvrđeno je da kratkoročno odnosno dugoročnije liječenje štakora melatoninom u različitoj mjeri štiti štakore istodobno izložene benzenu. Inhibicija lipidne peroksidacije mitohondrija i mikrosoma bila je različita nakon 24 h, 15 dana, odnosno 30 dana liječenja melatoninom. Najveća inhibicija lipidne peroksidacije mitohondrija zamijećena je nakon primjene jednokratne doze melatonina, dok je najizraženija inhibicija u mikrosomima zamijećena nakon 30 dana liječenja melatoninom. Slična istraživanja pokazuju da razina glutationa (GSH) najviše raste nakon 24 h liječenja melatoninom. Nije zamijećena razlika između liječenja u trajanju od 15 odnosno 30 dana. U štakora koji su uz benzen istodobno primali i melatonin razine citokroma P4502E1 pale su nakon 24 h odnosno 15 dana izloženosti. U štakora koji su primali samo melatonin te su razine nakon 30 dana statistički neznačajno porasle u odnosu na skupinu izloženu samo benzenu. Histopatološka analiza jetre načelno je potvrdila ove nalaze. Koncentracije fenola u mokraći bile su niže u štakora koji su istodobno primali melatonin i benzen. Ovi rezultati pokazuju da melatonin utječe na citokrom P4502E1, koji je odgovoran za metabolizam benzena. Inhibira li se njegov metabolizam, smanjuje se lipidna peroksidacija. Zaključak je da melatonin štiti od lipidne peroksidacije uzrokovane benzenom

Innate recognition of apoptotic cells:novel apoptotic cell-associated molecular patterns revealed by crossreactivity of anti-LPS antibodies

Cells dying by apoptosis are normally cleared by phagocytes through mechanisms that can suppress inflammation and immunity. Molecules of the innate immune system, the pattern recognition receptors (PRRs), are able to interact not only with conserved structures on microbes (pathogen-associated molecular patterns, PAMPs) but also with ligands displayed by apoptotic cells. We reasoned that PRRs might therefore interact with structures on apoptotic cells-apoptotic cell-associated molecular patterns (ACAMPs)-that are analogous to PAMPs. Here we show that certain monoclonal antibodies raised against the prototypic PAMP, lipopolysaccharide (LPS), can crossreact with apoptotic cells. We demonstrate that one such antibody interacts with a constitutively expressed intracellular protein, laminin-binding protein, which translocates to the cell surface during apoptosis and can interact with cells expressing the prototypic PRR, mCD14 as well as with CD14-negative cells. Anti-LPS cross reactive epitopes on apoptotic cells colocalised with annexin V-and C1q-binding sites on vesicular regions of apoptotic cell surfaces and were released associated with apoptotic cell-derived microvesicles (MVs). These results confirm that apoptotic cells and microbes can interact with the immune system through common elements and suggest that anti-PAMP antibodies could be used strategically to characterise novel ACAMPs associated not only with apoptotic cells but also with derived MVs

X-Ray Fluorescence Microscopy Reveals Accumulation and Secretion of Discrete Intracellular Zinc Pools in the Lactating Mouse Mammary Gland

The mammary gland is responsible for the transfer of a tremendous amount of zinc ( approximately 1-3 mg zinc/day) from maternal circulation into milk during lactation to support the growth and development of the offspring. When this process is compromised, severe zinc deficiency compromises neuronal development and immune function and increases infant morbidity and/or mortality. It remains unclear as to how the lactating mammary gland dynamically integrates zinc import from maternal circulation with the enormous amount of zinc that is secreted into milk.Herein we utilized X-ray fluorescence microscopy (XFM) which allowed for the visualization and quantification of the process of zinc transfer through the mammary gland of the lactating mouse. Our data illustrate that a large amount of zinc first accumulates in the mammary gland during lactation. Interestingly, this zinc is not cytosolic, but accumulated in large, discrete sub-cellular compartments. These zinc pools were then redistributed to small intracellular vesicles destined for secretion in a prolactin-responsive manner. Confocal microscopy identified mitochondria and the Golgi apparatus as the sub-cellular compartments which accumulate zinc; however, zinc pools in the Golgi apparatus, but not mitochondria are redistributed to vesicles destined for secretion during lactation.Our data directly implicate the Golgi apparatus in providing a large, mobilizable zinc storage pool to assist in providing for the tremendous amount of zinc that is secreted into milk. Interestingly, our study also provides compelling evidence that mitochondrial zinc pools expand in the mammary gland during lactation which we speculate may play a role in regulating mammary gland function