Znaczenie zjawiska alloreaktywności indukowanej infekcjami wirusowymi u chorych po przeszczepieniach allogenicznych krwiotwórczych komórek macierzystych

Viral infection activated alloreactive peripheral memory T-cells present in the host are considered as one of the causes of transplant related complications during post-transplant period of unrelated donor and haploidentical graft transplantation procedures. Antibody and T-cell receptor coding genes homology and their analogous rearrangement process help to understand cellular and humoral alloreactivity, mediated by alloreactive memory T-cells and antibodies, respectively. Simple tests using single antigen presenting cells or human leukocyte antigen (HLA)-mismatched recipient peripheral blood mononuclear cells vs. virus specific donor T-cells and more sophisticated natural killer cells degranulation marker assays based on flow cytometry enable to rule out or confirm alloreactivity thus increasing the chance of appropriate post-transplant treatment path selection.Krzyżowa odpowiedź alloreaktywnych limfocytów T pamięci aktywowanych infekcjami wirusowymi jest jedną z przyczyn powikłań u pacjentów poddawanych procedurze przeszczepienia krwiotwórczych komórek macierzystych od dawcy niespokrewnionego oraz przeszczepienia haploidentycznego. Homologia mechanizmów rearanżacji genów V(D)J kodujących receptory komórek T oraz część zmienną przeciwciał ułatwia zrozumienie zjawiska alloreaktywności komórkowej i humoralnej, w której pośredniczą odpowiednio limfocyty T pamięci wykazujące reaktywność krzyżową w stosunku do alloantygenów oraz alloreaktywne przeciwciała przeciw układowi ludzkich antygenów leukocytarnych. Proste testy oparte na pomiarze aktywacji komórek pamięci dawcy w kontakcie z komórkami prezentującymi niezgodny antygen oraz bardziej skomplikowane procedury oparte na cytometrycznym oznaczaniu markera degranulacji komórek naturalnej cytotoksyczności pozwalają pozyskać informacje o stopniu możliwych powikłań w przypadku alloreaktywnej odpowiedzi komórek biorcy na przeszczep

Tunneling-Induced Restoration of the Degeneracy and the Time-Reversal Symmetry Breaking in Optical Lattices

We study the ground-state properties of bosons loaded into the $p$-band of a one dimensional optical lattice. We show that the phase diagram of the system is substantially affected by the anharmonicity of the lattice potential. In particular, for a certain range of tunneling strength, the full many-body ground state of the system becomes degenerate. In this region, an additional symmetry of the system, namely the parity of the occupation number of the chosen orbital, is spontaneously broken. The state with nonvanishing staggered angular momentum, which breaks the time-reversal symmetry, becomes the true ground state of the system.Comment: 5 pages, 2 figure

Forecast of power generation and heat production from renewable energy sources

The share of renewable energy sources (RES) in the end use of energy in the UE will increase from the present level of about 25% to 50 % in 2030 according to the assumptions of the European Commission. In Poland the RES Act was passed in 2015. The act defines mechanisms and instruments for supporting the production of electricity and heat from renewable energy sources. Statistics (2003–2014) of electricity generation and heat production from RES in Poland were used in the research. Because of amendments to regulations connected with promoting RES and the emissions trading system (ETS) as well as the uncertainty associated with further directions of the energy and environmental policy, generation of electricity and heat based on the use of RES must be modelled while taking risk into account. A number of dynamic processes incorporating random events may be modelled by stochastic equations using Ito calculus. By applying Euler’s method to solve stochastic differential equations (SDE), it is possible to simulate the development of the use of renewable energy carriers in electricity generation and heat production in the future

Forecast of power generation and heat production from renewable energy sources

The share of renewable energy sources (RES) in the end use of energy in the UE will increase from the present level of about 25% to 50 % in 2030 according to the assumptions of the European Commission. In Poland the RES Act was passed in 2015. The act defines mechanisms and instruments for supporting the production of electricity and heat from renewable energy sources. Statistics (2003–2014) of electricity generation and heat production from RES in Poland were used in the research. Because of amendments to regulations connected with promoting RES and the emissions trading system (ETS) as well as the uncertainty associated with further directions of the energy and environmental policy, generation of electricity and heat based on the use of RES must be modelled while taking risk into account. A number of dynamic processes incorporating random events may be modelled by stochastic equations using Ito calculus. By applying Euler’s method to solve stochastic differential equations (SDE), it is possible to simulate the development of the use of renewable energy carriers in electricity generation and heat production in the future

Forecast of power generation and heat production from renewable energy sources

The share of renewable energy sources (RES) in the end use of energy in the UE will increase from the present level of about 25% to 50 % in 2030 according to the assumptions of the European Commission. In Poland the RES Act was passed in 2015. The act defines mechanisms and instruments for supporting the production of electricity and heat from renewable energy sources. Statistics (2003–2014) of electricity generation and heat production from RES in Poland were used in the research. Because of amendments to regulations connected with promoting RES and the emissions trading system (ETS) as well as the uncertainty associated with further directions of the energy and environmental policy, generation of electricity and heat based on the use of RES must be modelled while taking risk into account. A number of dynamic processes incorporating random events may be modelled by stochastic equations using Ito calculus. By applying Euler’s method to solve stochastic differential equations (SDE), it is possible to simulate the development of the use of renewable energy carriers in electricity generation and heat production in the future

Conformational tuning improves the stability of spirocyclic nitroxides with long paramagnetic relaxation times

Nitroxides are widely used as probes and polarization transfer agents in spectroscopy and imaging. These applications require high stability towards reducing biological environments, as well as beneficial relaxation properties. While the latter is provided by spirocyclic groups on the nitroxide scaffold, such systems are not in themselves robust under reducing conditions. In this work, we introduce a strategy for stability enhancement through conformational tuning, where incorporating additional substituents on the nitroxide ring effects a shift towards highly stable closed spirocyclic conformations, as indicated by X-ray crystallography and DFT calculations. Closed spirocyclohexyl nitroxides exhibit a dramatically improved stability towards reduction by ascorbate, while maintaining long relaxation times in EPR spectroscopy. These findings have important implications for the future design of new nitroxide-based spin labels and imaging agents

Conformational tuning improves the stability of spirocyclic nitroxides with long paramagnetic relaxation times

Abstract Nitroxides are widely used as probes and polarization transfer agents in spectroscopy and imaging. These applications require high stability towards reducing biological environments, as well as beneficial relaxation properties. While the latter is provided by spirocyclic groups on the nitroxide scaffold, such systems are not in themselves robust under reducing conditions. In this work, we introduce a strategy for stability enhancement through conformational tuning, where incorporating additional substituents on the nitroxide ring effects a shift towards highly stable closed spirocyclic conformations, as indicated by X-ray crystallography and density functional theory (DFT) calculations. Closed spirocyclohexyl nitroxides exhibit dramatically improved stability towards reduction by ascorbate, while maintaining long relaxation times in electron paramagnetic resonance (EPR) spectroscopy. These findings have important implications for the future design of new nitroxide-based spin labels and imaging agents

MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells

Cancer cells show significant dysregulation of genes expression, which may favor their survival in the tumor environment. In this study, the cellular vault’s components MVP (major vault protein), TEP1 (telomerase-associated protein 1) and vPARP (vault poly(ADP-ribose) polymerase) were transiently or completely inhibited in U2OS cells (human bone osteosarcoma epithelial cells) to evaluate their impact on the cell proliferative and migratory capacity as well as on the development of their resistance to the drug vinorelbine. Comparative analysis of MVP protein expression level in normal colon tissue, primary colorectal tumor, and metastasis showed that the expression of this protein does not increase significantly in the primary tumor, but its expression increases in metastatic cells. Further comparative molecular analysis using the whole transcriptome microarrays for MVP-positive and MVP-negative cells showed that MVP is involved in regulating proliferation and migration of cancer cells. MVP may facilitate metastasis of colon cancer due to its impact on cell migration. Moreover, two vault proteins, MVP and TEP1, contribute the resistance to vinorelbine, while vPARP does not

Replication Data for: Conformational tuning improves the stability of spirocyclic nitroxides with long paramagnetic relaxation times

Nitroxides are widely used as probes and polarization transfer agents in spectroscopy and imaging.These applications require high stability towards reducing biological environments, as well as beneficial relaxation properties. Closed spirocyclohexyl nitroxides exhibit a dramatically improved stability towards reduction by ascorbate, while maintaining long relaxation times in EPR spectroscopy. This dataset contains raw files and analysis reports of 1HNMR, 13CNMR, HRMS, FTIR of all compounds synthesized for and used in work: Conformational tuning improves the stability of spirocyclic nitroxides with long paramagnetic relaxation times. Moreover, for nitroxides raw files of X-band CW-EPR spectra, kinetic runs based on CW-EPR spectra and Q-band EPR relaxation measurements are included. Dataset is completed with X-ray crystallography CIF files and reports