Uji aktivitas antioksidan ekstrak ultrasonik air, metanol, etanol, etil asetat dan petroleum eter daun katuk (sauropus androgynus l.merr)

Indonesia : Katuk (Sauropus androgynus L. Merr) merupakan tumbuhan yang sering dimanfaatkan oleh masyarakat sebagai tanaman obat. Tumbuhan ini mengandung senyawa metabolit sekunder yang mengakibatkan adanya aktivitas farmakologis yang beragam. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antioksidan daun Katuk pada variasi pelarut dengan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH) dan untuk mengetahui golongan senyawa metabolit sekunder daun Katuk yang dapat memberikan aktivitas antioksidan. Ekstraksi senyawa metabolit sekunder daun Katuk dilakukan dengan metode ultrasonik menggunakan variasi pelarut air, metanol, etanol, etil asetat dan petroleum eter. Ekstrak yang diperoleh dari masing-masing pelarut diuji aktivitas antioksidannya dengan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH). Identifikasi senyawa aktif daun Katuk dilakukan dengan uji fitokimia, Kromatografi Lapis Tipis Analitik (KLTA) dan spektrofotometer FT-IR. Hasil dari uji aktivitas antioksidan daun Katuk menunjukkan hasil tertinggi pada ekstrak etil asetat daun Katuk dengan nilai IC50 43,67 ppm. Sedangkan aktivitas antioksidan ekstrak air, metanol, etanol dan petroleum eter daun Katuk menghasilkan nilai IC50 berturut-turut 1624; 829,8; 329,9; 43,67 dan 75,39 ppm. Hasil dari uji fitokimia daun Katuk menunjukkan adanya senyawa alkaloid, flavonoid, tanin, triterpenoid dan steroid. Identifikasi menggunakan FTIR menunjukkan adanya serapan gugus fungsi O-H, -CH3, -CH2, C=C dan C-N. English : Katuk (Sauropus androgynus L. Merr) is a plant that is often used by the community as a medicinal plant. This plant contains secondary metabolites which cause various pharmacological activities. The purpose of this experiment is to determine the antioxidant activity of Katuk leaves in variety of solvents with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and to determine the class of Katuk leaves secondary metabolite compounds that can provide antioxidant activity. The extraction of secondary metabolites from Katuk leaves was carried out by ultrasonic methods using variations of water, methanol, ethanol, ethyl acetate and petroleum ether as solvents. The extracts obtained from each solvent were tested for their antioxidant activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Identification of the active compound Katuk leaves was carried out by phytochemical tests, Analytical Thin Layer Chromatography (KLTA) and FT-IR spectrophotometer. The results of the antioxidant activity of Katuk leaves showed the highest yield on the ethyl acetate extract of Katuk leaves with an IC50 value of 43.67 ppm. While the antioxidant activity of the extracts of water, methanol, ethanol and petroleum ether of Katuk leaves resulted in IC50 values of 1624; 829.8; 329.9; 43.67 dan 75.39 ppm. The results of the phytochemical test of Katuk leaves showed the presence of alkaloids, flavonoids, tannins, triterpenoids and steroids. Identification using FTIR shows the absorption of functional groups O-H, -CH3, -CH2, C=C and C-N. Arabic : كاتوك (Sauropus androgynus L. Merr) هو نبات يستخدمه المجتمع غالبًا كنبات طبي. يحتوي هذا النبات على مركبات مستقلب ثانوية تؤدي إلى أنشطة دوائية مختلفة. كان الغرض من هذه الدراسة هو تحديد النشاط المضاد للأكسدة لأوراق الكاتوك في المذيبات المختلفة باستخدام طريقة 1,1-diphenyl-2-picrylhydrazyl (DPPH) وتحديد فئة المستقلبات الثانوية لأوراق الكاتوك التي يمكن أن توفر نشاطا مضادا للأكسدة. تم استخلاص المستقلبات الثانوية من أوراق كاتوك باستخدام طرق الموجات فوق الصوتية باستخدات أشكال مختلفة من الماء و الميثانول و خلات الإيثيل و الإيثرولي كمذيبات. المذيبات المستخدمة هي الماء والميثانول والأيثانول وخلات الإيثيل وإيثر البترول. تم اختبار المستخلص الذي تم الحصول عليه من كل مذيب لمعرفة نشاطه المضاد للأكسدة باستخدام طريقة 1,1-diphenyl-2-picrylhydrazyl (DPPH).تم التعرف على المركب الفعال أوراق كاتوك عن طريق الاختبارات الكيميائية النباتية، التحليل اللوني للطبقة الرقيقة (TCL) و مقياس الطيف الضوئي FTIR. أظهرت نتائج النشاط المضاد للأكسدة لأوراق الكاتوك أعلى إنتاجية على مستخلص أسيتات الإيثيل لأوراق الكاتوك بقيمة IC50 43،67 جزء في المليون. بينما أدى النشاط المضاد للأكسدة لمستخلصات الماء والميثانول والإيثانول والإيثر البترولي لأوراق كاتوك إلى قيم IC50 قدرها 1624؛ 829،8؛ 329،9 و 75،39 جزء في المليون. أظهرت نتائج الاختبار الكيميائي النباتي لأوراق الكاتوك وجود قلويدات وفلافونويد وعفص وتريتربينويد وسترويدات. يُظهر تحديد الهوية باستخدام FTIR امتصاص المجموعات الوظيفية O-H, -CH3, CH2, C=C و C-N

Antioxidant activity and toxicity test of column chromatography steroids isolates from Hydrilla verticillata chloroform fraction

Hydrilla verticillata contain some secondary metabolites, such as steroids and triterpenoids. The purpose of this study was to determine antioxidant activity and the toxicity levels of steroids isolates from Hydrilla verticillata chloroform fraction. The biomass of Hydrilla verticillata was dried and then powdered. The powder of Hydrilla verticillata was extracted by maceration methods using ethanol as a solvent. The crude ethanol extracts were hydrolyzed with 2 N HCl before partitioned using chloroform. The steroid compounds were separated by Column Chromatography. Antioxidant activities of Column Chromatography steroid isolates were determined by the 2,2-diphenyl-1-picrylhydrazil (DPPH) method, and the toxicity levels were determined by the Brine Shrimp Lethality Test (BSLT) method. The result showed that extraction by maceration with ethanol produced 2.52 percent yield, whereas the percent yield of the partition with chloroform was 18.48 percent. Separation by column chromatography obtained four steroids isolates B1, B2, B2G1, and G1R1. The steroids isolates of chloroform fraction of Hydrilla verticillata have antioxidant activity and toxicity properties. The EC50 value of the isolates B1, B2, B2G1, and G1R1 were 5375, 179.40, 65.97, and 6.55 ppm, respectively. The LC50 value of B1, B2, B2G1, and G1R1 isolate were 5.99, 3.86, 6.86, and 4.37 ppm, respectivel

Antioxidant activity and toxicity test of column chromatography steroids isolates from Hydrilla verticillata chloroform fraction

Hydrilla verticillata contain some secondary metabolites, such as steroids and triterpenoids. The purpose of this study was to determine antioxidant activity and the toxicity levels of steroids isolates from Hydrilla verticillata chloroform fraction. The biomass of Hydrilla verticillata was dried and then powdered. The powder of Hydrilla verticillata was extracted by maceration methods using ethanol as a solvent. The crude ethanol extracts were hydrolyzed with 2 N HCl before partitioned using chloroform. The steroid compounds were separated by Column Chromatography. Antioxidant activities of Column Chromatography steroid isolates were determined by the 2,2-diphenyl-1-picrylhydrazil (DPPH) method, and the toxicity levels were determined by the Brine Shrimp Lethality Test (BSLT) method. The result showed that extraction by maceration with ethanol produced 2.52 percent yield, whereas the percent yield of the partition with chloroform was 18.48 percent. Separation by column chromatography obtained four steroids isolates B1, B2, B2G1, and G1R1. The steroids isolates of chloroform fraction of Hydrilla verticillata have antioxidant activity and toxicity properties. The EC50 value of the isolates B1, B2, B2G1, and G1R1 were 5375, 179.40, 65.97, and 6.55 ppm, respectively. The LC50 value of B1, B2, B2G1, and G1R1 isolate were 5.99, 3.86, 6.86, and 4.37 ppm, respectivel