96 research outputs found
Tunable few-electron double quantum dots and Klein tunnelling in ultra-clean carbon nanotubes
Quantum dots defined in carbon nanotubes are a platform for both basic
scientific studies and research into new device applications. In particular,
they have unique properties that make them attractive for studying the coherent
properties of single electron spins. To perform such experiments it is
necessary to confine a single electron in a quantum dot with highly tunable
barriers, but disorder has until now prevented tunable nanotube-based
quantum-dot devices from reaching the single-electron regime. Here, we use
local gate voltages applied to an ultra-clean suspended nanotube to confine a
single electron in both a single quantum dot and, for the first time, in a
tunable double quantum dot. This tunability is limited by a novel type of
tunnelling that is analogous to that in the Klein paradox of relativistic
quantum mechanics.Comment: 21 pages including supplementary informatio
JuPOETs: a constrained multiobjective optimization approach to estimate biochemical model ensembles in the Julia programming language
Carbon nanotubes for coherent spintronic devices
Carbon nanotubes bridge the molecular and crystalline quantum worlds, and
their extraordinary electronic, mechanical and optical properties have
attracted enormous attention from a broad scientific community. We review the
basic principles of fabricating spin-electronic devices based on individual,
electrically-gated carbon nanotubes, and present experimental efforts to
understand their electronic and nuclear spin degrees of freedom, which in the
future may enable quantum applications.Comment: 17 pages, 9 figures, submitted to Materials Toda
Meta learning addresses noisy and under-labeled data in machine learning-guided antibody engineering
ISSN:2405-472
Nucleotide augmentation for machine learning-guided protein engineering
SUMMARY: Machine learning-guided protein engineering is a rapidly advancing field. Despite major experimental and computational advances, collecting protein genotype (sequence) and phenotype (function) data remains time- and resource-intensive. As a result, the quality and quantity of training data are often a limiting factor in developing machine learning models. Data augmentation techniques have been successfully applied to the fields of computer vision and natural language processing; however, there is a lack of such augmentation techniques for biological sequence data. Towards this end, we develop nucleotide augmentation (NTA), which leverages natural nucleotide codon degeneracy to augment protein sequence data via synonymous codon substitution. As a proof of concept for protein engineering, we test several online and offline augmentation implementations to train machine learning models with benchmark datasets of protein genotype and phenotype, revealing performance gains on par and surpassing benchmark models using a fraction of the training data. NTA also enables substantial improvements for classification tasks under heavy class imbalance. AVAILABILITY AND IMPLEMENTATION: The code used in this study is publicly available at https://github.com/minotm/NTA SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics Advances online
Reduced order modeling and analysis of the human complement system
<div><p>Complement is an important pathway in innate immunity, inflammation, and many disease processes. However, despite its importance, there are few validated mathematical models of complement activation. In this study, we developed an ensemble of experimentally validated reduced order complement models. We combined ordinary differential equations with logical rules to produce a compact yet predictive model of complement activation. The model, which described the lectin and alternative pathways, was an order of magnitude smaller than comparable models in the literature. We estimated an ensemble of model parameters from <i>in vitro</i> dynamic measurements of the C3a and C5a complement proteins. Subsequently, we validated the model on unseen C3a and C5a measurements not used for model training. Despite its small size, the model was surprisingly predictive. Global sensitivity and robustness analysis suggested complement was robust to any single therapeutic intervention. Only the simultaneous knockdown of both C3 and C5 consistently reduced C3a and C5a formation from all pathways. Taken together, we developed a validated mathematical model of complement activation that was computationally inexpensive, and could easily be incorporated into pre-existing or new pharmacokinetic models of immune system function. The model described experimental data, and predicted the need for multiple points of therapeutic intervention to fully disrupt complement activation.</p></div
Global sensitivity analysis of the reduced order complement model.
<p>Sensitivity analysis was conducted on the two objectives used for model training. <b>A</b>: Sensitivity of the C3a and C5a residual w/o zymosan. <b>B</b>: Sensitivity of the C3a and C5a residual with 1 mg/ml zymosan. The bars denote the mean total sensitivity index for each parameter, while the error bars denote the 95% confidence interval. <b>C</b>: Pathways controlled by the sensitivity parameters. Bold black lines indicate the pathway involves one or more sensitive parameters, while the red lines show current therapeutics targets. Current complement therapeutics were taken from the review of Morgan and Harris [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0187373#pone.0187373.ref039" target="_blank">39</a>].</p
Simplified schematic of the human complement system.
<p>The complement cascade is activated through three pathways: the classical, the lectin, and the alternative pathways. Complement initiation results in the formation of classical or alternative C3 convertases, which amplify the initial complement response and signal to the adaptive immune system by cleaving C3 into C3a and C3b. The C3 convertases further react to form C5 convertases which catalyze the cleavage of the C5 complement protein to C5a and C5b. C5b is critical to the formation of the membrane attack complex (MAC), while C5a recruits an adaptive immune response.</p
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