Georadar investigations to detect cavities in a historical town damaged by an earthquake of the past

This paper aims to highlight the use of the georadar as a useful prospecting technique to identify the areal density and the geometrical features of the grottoes placed in a historical town characterised by high seismic hazard. <br><br> The town considered here is Rionero in Vulture (Southern Italy) that was hit by several historical earthquakes, among which the 1930 Irpinia earthquake (Me=6.7, Is=VIII MCS). <br><br> For this event a damage map was already available from a previous study (Gizzi and Masini, 2006). This map shows that some sectors of the town suffered higher damage. One factor causing the uneven distribution of the effects is considered to be the presence of grottoes. <br><br> To strengthen this work hypothesis it was necessary to in-depth investigate the subsoil of Rionero in Vulture. Therefore, geophysical data were correlated and integrated with data obtained from field surveys and historical documentary sources. All these investigations allowed to obtain more insights about the influences of the man-made caves on seismic damage

Multi-Scale Monitoring of Rupestrian Heritage: Methodological Approach and Application to a Case Study

Most of the artistic heritage in the Mediterranean basin is hosted in rupestrian hypogeum whose peculiarity is given by the presence of at least one open side, which makes them particularly sensitive to meteorological conditions. This makes mandatory the monitoring of both indoor and outdoor environmental parameters to analyze the cause–effect relationship between microclimatic inside and outside the hypogeum. The paper proposes a spatial and temporal multi-scale methodological approach applied to a rupestrian church in Matera, which hosts precious wall paintings, particularly vulnerable to the effects of environmental parameters. The approach is based on the analysis of data acquired by three platforms: indoor, close-range outdoor, and outdoor data from a meteorological station and weather forecast from the COSMO 5 model. The method allowed to characterize the relationships between the indoor and outdoor parameters at different spatial and temporal scales. The results showed a significant correlation between the parameters, thus opening new opportunities for the monitoring of the rupestrian heritage based on the use of data systematically available, such as those from meteorological stations and meteorological forecast

A growth-rate indicator for Compton-thick active galactic nuclei

Due to their heavily obscured central engines, the growth rate of Compton-thick (CT) active galactic nuclei (AGN) is difficult to measure. A statistically significant correlation between the Eddington ratio, {\lambda}$_{Edd}$, and the X-ray power-law index, {\Gamma}, observed in unobscured AGN offers an estimate of their growth rate from X-ray spectroscopy (albeit with large scatter). However, since X-rays undergo reprocessing by Compton scattering and photoelectric absorption when the line-of-sight to the central engine is heavily obscured, the recovery of the intrinsic {\Gamma} is challenging. Here we study a sample of local, predominantly Compton-thick megamaser AGN, where the black hole mass, and thus Eddington luminosity, are well known. We compile results on X-ray spectral fitting of these sources with sensitive high-energy (E> 10 keV) NuSTAR data, where X-ray torus models which take into account the reprocessing effects have been used to recover the intrinsic {\Gamma} values and X-ray luminosities, L$_X$. With a simple bolometric correction to L$_X$ to calculate {\lambda}$_{Edd}$, we find a statistically significant correlation between {\Gamma} and {\lambda}$_{Edd}$ (p = 0.007). A linear fit to the data yields {\Gamma} = (0.41$\pm$0.18)log$_{10}${\lambda}$_{Edd}$+(2.38$\pm$ 0.20), which is statistically consistent with results for unobscured AGN. This result implies that torus modeling successfully recovers the intrinsic AGN parameters. Since the megamasers have low-mass black holes (M$_{BH}\approx10^6-10^7$ M$_{sol}$) and are highly inclined, our results extend the {\Gamma}-{\lambda}$_{Edd}$ relationship to lower masses and argue against strong orientation effects in the corona, in support of AGN unification. Finally this result supports the use of {\Gamma} as a growth-rate indicator for accreting black holes, even for Compton-thick AGN.Comment: Accepted for publication in Ap

Measuring and explaining the diversity of voices and viewpoints in the news

News media can be considered to fulfil their democratic role as a “marketplace of ideas” only if they present a diverse content that gives space to a wider range of ideas and viewpoints. But how can content diversity be assessed? And what determines actor and viewpoint diversity in the first place? By employing measurements of actor and viewpoint diversity at the article and newspaper level, this study provides a complete overview on the content diversity of immigration news, and it investigates factors that have an impact on content diversity of immigration newspaper articles in Belgium, Germany, Italy and the United Kingdom (2013-2014). The results of a multilevel analysis indicate that both the articles’ size and the elite character of a newspaper play a key role in enhancing news’ multiperspectivalness. Also, the findings show that these two measurements of content diversity are different yet related to each other

Zymographic assay of plant diamine oxidase on entrapped peroxidase polyacrylamide gel electrophoresis. A study of stability to proteolysis

A zymographic assay of diamine oxidase (DAO, histaminase, EC 1.4.3.6), based on a coupled peroxidase reaction, and its behavior at proteolysis in simulated gastric and intestinal conditions, are described. The DAO activity from a vegetal extract of Lathyrus sativus seedlings was directly determined on sodium dodecyl sulfate polyacrylamide electrophoretic gels containing entrapped horseradish peroxidase, with putrescine as substrate of histaminase and ortho-phenylenediamine as co-substrate of peroxidase. The accumulation of azo-aniline, as peroxidase-catalyzed oxidation product, led to well-defined yellow-brown bands on gels, with intensities corresponding to the enzymatic activity of DAO. After image analysis of gels, a linear dependency of DAO content (Coomassie-stained protein bands) and of its enzymatic activity (zymographic bands) with the concentration of the vegetal extract was obtained. In simulated gastric conditions (pH 1.2, 37 °C), the DAO from the vegetal extract lost its enzymatic activity before 15 min of incubation, either in the presence or absence of pepsin. The protein pattern (Coomassie-stained) revealed that the DAO content from the vegetal extract was kept almost constant in the simulated intestinal fluid (containing pancreatin or not), with a slight diminution in the presence of pancreatic proteases. After 10 h of incubation at 37 °C, the DAO enzymatic activity from the vegetal extract was 44.7% in media without pancreatin and 13.6% in the presence of pancreatin, whereas the purified DAO retained only 4.65% of its initial enzymatic activity in the presence of pancreatin

Supraspinal inactivation of mitochondrial superoxide dismutase is a source of peroxynitrite in the development of morphine antinociceptive tolerance.

Effective treatment of chronic pain with morphine is limited by decreases in the drug’s analgesic action with chronic administration (antinociceptive tolerance). Because opioids are mainstays of pain management, restoring their efficacy has great clinical importance. We have recently reported that formation of peroxynitrite (ONOO(−), PN) in the dorsal horn of the spinal cord plays a critical role in the development of morphine antinociceptive tolerance and have further documented that nitration and enzymatic inactivation of mitochondrial superoxide dismutase (MnSOD) at that site provides a source for this nitroxidative species. We now report for the first time that antinociceptive tolerance is also associated with the inactivation of MnSOD at supraspinal sites. Inactivation of MnSOD led to nitroxidative stress as evidenced by increased levels of products of oxidative DNA damage and activation of the nuclear factor poly (ADP-ribose) polymerase in whole brain homogenates. Co-administration of morphine with potent Mn porphyrin-based peroxynitrite scavengers, (MnTE-2-PyP(5+) and MnTnHex-2-PyP(5+)) (1) restored the enzymatic activity of MnSOD, (2) attenuated PN derived nitroxidative stress, and (3) blocked the development of morphine induced antinociceptive tolerance. The more lipophilic analogue, MnTnHex-2-PyP(5+) was able to cross the blood brain barrier at higher levels than its lipophylic counterpart MnTE-2-PyP(5+) and was about 30 fold more efficacious. Collectively, these data suggest that peroxynitrite mediated enzymatic inactivation of supraspinal MnSOD provides a source of nitroxidative stress, which in turn contributes to central sensitization associated with the development of morphine antinociceptive tolerance. These results support our general contention that PN-targeted therapeutics may have potential as adjuncts to opiates in pain management

A new approach to antiglaucoma drugs: carbonic anhydrase inhibitors with or without NO donating moieties. Mechanism of action and preliminary pharmacology.

The clinically used sulfonamide carbonic anhydrase (CA, EC 4.2.1.1) inhibitor dorzolamide (DRZ), a new sulfonamide CA inhibitor also incorporating NO-donating moieties, NCX250, and isosorbide mononitrate (ISMN) (an NO-donating compound with no CA inhibitory properties) were investigated for their intraocular pressure (IOP) lowering effects in rabbits with carbomer-induced glaucoma. NCX250 was more effective than DRZ or ISMN on lowering IOP, increasing ocular hemodynamics, decreasing the inflammatory processes and ocular apoptosis in this animal model of glaucoma. NO participate to the regulation of IOP in glaucoma, having also antiapoptotic and anti-inflammatory effects. The ophthalmic artery, both systolic and diastolic velocities, were significantly reduced in NCX250-treated eyes in comparison to DRZ treated ones, suggesting thus a beneficial effect of NCX250 on the blood supply to the optic nerve. Combining CA inhibition with NO-donating moieties in the same compound offers an excellent approach for the management of glaucoma

COVID-19 and MIS-C treatment in children—results from an international survey

Children have been mostly excluded from COVID-19 clinical trials, and, as a result, most medicines approved for COVID-19 have no pediatric indication. In addition, access to COVID-19 therapeutics remains limited. Collecting physicians’ experiences with off-label use of therapeutics is important to inform global prioritization processes and better target pediatric research and development. A standardized questionnaire was designed to explore the use of therapeutics used to treat COVID-19 and multisystem inflammatory syndrome in children (MIS-C) in pediatric patients globally. Seventy-three physicians from 29 countries participated. For COVID-19, steroids were used by 75.6% of respondents; remdesivir and monoclonal antibodies were prescribed by 48.6% and 27.1% of respondents, respectively. For MIS-C, steroids were prescribed by 79.1% of respondents and intravenous immunoglobulins by 69.6%. The use of these products depended on their pediatric approval and the limited availability of antivirals and most monoclonal antibodies in Africa, South America, Southeast Asia, and Eastern Europe. Off-label prescription resulted widespread due to the paucity of clinical trials in young children at the time of the survey; though, based on our survey results, it was generally safe and led to clinical benefits. Conclusion: This survey provides a snapshot of current practice for treating pediatric COVID-19 worldwide, informing global prioritization efforts to better target pediatric research and development for COVID-19 therapeutics. Off-label use of such medicines is widespread for the paucity of clinical trials under 12 years and 40 kg, though appears to be safe and generally results in clinical benefits, even in young children. However, access to care, including medicine availability, differs widely globally. Clinical development of COVID-19 antivirals and monoclonal antibodies requires acceleration to ensure pediatric indication and allow worldwide availability of therapeutics that will enable more equitable access to COVID-19 treatment. What is Known: • Children have been mostly excluded from COVID-19 clinical trials, and, as a result, most medicines approved for COVID-19 have no pediatric indication. • Access to care differs widely globally, so because of the diversity of national healthcare systems; the unequal availability of medicines for COVID-19 treatment represents an obstacle to the pediatric population's universal right to health care. What is New: • Off-label COVID-19 drug prescription is widespread due to the lack of clinical trials in children younger than 12 years and weighing less than 40 kg, but relatively safe and generally leading to clinical benefit. • The application of the GAP-f framework to COVID-19 medicines is crucial, ensuring widespread access to all safe and effective drugs, enabling the rapid development of age-appropriate formulations, and developing specific access plans (including stability, storage, packaging, and labeling) for distribution in low- and middle-income countries (LMICs). Antivirals and monoclonal antibodies may benefit from the acceleration to reach widespread and equal diffusion

Self-reported face recognition abilities moderately predict face-learning skills: Evidence from Italian samples

Face Recognition Ability (FRA) varies widely throughout the population. Previous research highlights a positive relationship between self-perceived and objectively measured FRA in the healthy population, suggesting that people do have insight into their FRA. Given that this relationship has not been investigated in Italian samples yet, the main aim of the present work was to develop an Italian translation of the Prosopagnosia Index-20 (PI-20), a self-report measure of FRA, to investigate the relationship between PI-20 performances and an objective assessment given by the Cambridge Face Memory Test Long Form (CFMT+) in the Italian population. A sample of 553 participants filled in the PI-20 Italian version 1 or 2 (PI-20_GE or PI-20_BA) and completed the CFMT+. Results showed a negative correlation between both versions of the Italian PI-20 and CFMT+ scores, meaning that the more self-evaluations were negative, the worse they objectively performed. The same results applied to the extreme limits of the distribution (i.e., 10% of the highest and lowest PI-20 scores). Furthermore, both age and administration order of the tests were predictor variables of CFMT+ scores. Overall, our results suggest that people possess insight, although relatively limited, into their FRA

Prone versus supine position for adjuvant breast radiotherapy: a prospective study in patients with pendulous breasts.

Purpose: To analyze dosimetric parameters of patients receiving adjuvant breast radiotherapy (RT) in the prone versus supine position. Methods and materials: Forty-one out of 55 patients with pendulous breasts and candidates for adjuvant RT were enrolled in the study after informed consent. They underwent computed tomography (CT)-simulation in both prone and supine position. Target and non target volumes were outlined on CT images. Prescribed dose was 50 Gy delivered by two tangential photon fields followed by 10 Gy electron boost. Target coverage and dose homogeneity to clinical target volume (CTV) and planning target volume (PTV) were assessed by V95, V105 and V107 and dose to lung, heart and left anterior descending coronary artery (LAD) by V5, V10, V20, and mean and maximum dose. Data were analyzed by Student\u2019s t-test. Results: CTV and PTV coverage was significantly better in supine than in prone position. Lung V5, V10, and V20 were significantly lower in prone than in supine position. Heart V5, V10, V20, and LAD mean and maximum dose, in the 17 patients with left breast tumor, were lower in prone than in supine position, but without statistical significance. Based on treatment planning data and on treatment feasibility, 29/41 patients (70.7%) were treated in prone position. Acute and late toxicities of patients treated in prone and in supine position were not statistically different. Conclusion: Prone position is a favorable alternative for irradiation of mammary gland in patients with pendulous breasts and in our series was adopted in 71% of the cases