Cell Cycle Control Gene-Gene Interaction Model in Pregnancy Loss

Early stages of individual development are defined by active cell division and differentiation processes. Intercellular signaling molecules as well as proliferation control and DNA stabilizing factors act as basic functional groups. Changes in genome stability maintaining genes functioning can cause the mutation number increase during blastomeric cleavage and differentiation. Thereby the goal of current work was to investigate the frequencies of cell cycle and DNA reparation control genes polymorphic variants in women with normally progressing pregnancies and in women with first trimester pregnancy loss. The present case-control study included 151 women with spontaneous abortion and 134 women with normally progressing pregnancies. Polymorphisms Pro72Arg in TP53, Asp148Glu in APEX1 and Lys751Gln in ERCC2 were detected by allele-specific PCR. When analyzed separately, the allele and genotype distributions of the polymorphisms in the two groups were similar. However, in MDR analysis the interaction between the genotypes TP53, APEX1, XPD showed to be associated to early pregnancy loss

Vascular Endothelial Growth Factor: Genetic Aspects in Children with Asthma in the Rostov Region

Vascular endothelial growth factor (VEGF) plays an important role in the pathogenesis of asthma. It activates neoangiogenesis, thus contributing to disruption of the microcirculation in the bronchi and increasing the intensity of exposure to the elements of the inflammatory cascade. So, the goal of this research was to evaluate the role of C-634G VEGFA gene polymorphism in the pathogenesis of childhood asthma. In the analysis of the blood no statistically significant differences has been revealed in the genotype frequencies and allele on investigated polymorphism between the control group of children and the group of children with asthma. The most frequently registered one is homozygous polymorphism of VEGFA gene. The influence of the polymorphic C-634G of VEGFA gene on the concentration of serum IgE is not revealed

Transforming Growth Factor Β: Clinical Significance and Peculiarities of Inheritance in Children with Atopic Dermatitis

Transforming growth factor β1 (TGF-β1) plays an important role in the pathogenesis of many allergic diseases, including atopic dermatitis (AD). Objective: to study the role of TGF-β1 in the pathogenesis of AD in children. Materials and methods. A survey was conducted of 34 patients with AD and 20 healthy children. TGF-β1 in serum were determined by ELISA using kits of Human TGF beta1 Platinum ELISA. Definition Arg25Pro gene polymorphism of TGF β1 was carried out using allele-specific polymerase chain reaction. Results. It was found that patients with AD showed a significant increase in the concentration of TGF-β in the serum (222,37±68,58 PG/ml) compared with the control group (3,03±0,41 ng/ml) [p = 0.001]. The concentration depends on the severity and duration of the illness. Frequency of genotypes for the polymorphism Arg25Pro TGF β1 gene among patients with AD had significant differences from the control group (p < 0.05). Moreover, among patients who are heterozygotes for the gene Arg25Pro TGF β1, was significantly more observed moderate (77.78 %) and severe (33,33%) the course of the disease. Conclusion. The obtained results confirm the importance of TGF-β1 in the pathogenesis of AD

GDF15 in the Physiological Human Pregnancy: Novel Data and A Systematic Review

The involvement of GDF15 protein in modulating the processes of cellular migration and adhesion, tissue remodeling and inflammatory responses suggests that this factor may be of high importance for pregnancy establishment and its further maintenance. Analyzing pregnancy-associated dynamics of the factor confers a valuable addition to the case-control studies and can help advance our understanding of the fundamentals of the factor’s involvement in pregnancy. However, as seen from the systematic literature review presented in this paper, current data set significant questions on roles of GDF15 in pregnancy. Additionally, a significant controversy is seen in the literature regarding the gestational dynamics of GDF15. Thus, we tried to contribute to the field by assessing the dynamics of serum GDF15 protein in the course of the physiological human pregnancy. We found that serum maternal GDF15 levels increase by almost two orders of magnitude with progression of pregnancy from the mid-first trimester towards the early second trimester and then towards the term. These data were further compared with those found in the literature