The effects of general anaesthesia on heart rate variability during abdominal surgery

We aimed to realized a pilot study that investigated heart rate variability (HRV) during anesthesia to study of alterations in the autonomous function and study the effects of anesthetic drugs with a not invasive test. We studied 15 subjects of both sexes (9 women and 6 men) with a mean age of 53.6 ± 14.3 years. ECG signal recording, lasting 5 minutes each, in three times: First time, the    registration before anaesthesia. The second was performed after anaesthesia induction and after 5 minutes after the start of maintenance. The third measurement was  performed 24 hours after surgery. The mean heart rate did not show significant alterations during anesthesia and after 24 hours of surgery compared with baseline. On the contrary, the indices of heart rate variability in the frequency domain showed significant variations during general anesthesia. In fact there was a significant decrease in LF, expressed in normalized units and at the same time a significant increase in HF, always expressed in normalized units. It follows that the LF / HF ratio has been significantly reduced during the period of anesthesia. All indices are nearly returned to baseline after 24 hours of surgery. The analysis of anesthetic effects on HRV may provide a more valuable noninvasive tool for investigating alterations in autonomic function. Anesthetics used in general anesthesia suppress the autonomic nervous system and contribute to the safety of general anesthesia not only because suppress the excessive sympathetic activity caused by the operation but also because suppress parasympathetic reactions. The attenuation of sympathetic activity during general anesthesia is usually assessed by measuring changes in blood pressure or heart rate. In all cases, because of these antagonistic effects, evaluation becomes problematic when parasympathetic activity is simultaneously depressed. Keywords: HRV; heart rate variability; general anaesthesia; autonomic nervous system

Giving Ideas Some Legs or Legs Some Ideas? Children's Motor Creativity Is Enhanced by Physical Activity Enrichment: Direct and Mediated Paths.

Approaches to foster motor creativity differ according to whether creative movements are assumed to be enacted creative ideas, or solutions to emerging motor problems that arise from task and environmental constraints. The twofold aim of the current study was to investigate whether (1) an enriched physical education (PE) intervention delivered with a joint constraints-led and cognitive stimulation approach fosters motor creativity, and the responsiveness to the intervention is moderated by baseline motor and cognitive skills and sex; (2) the intervention may benefit motor creativity through gains in motor coordination, executive function, and creative thinking. Ninety-five children, aged 6-9 years, participated in a 6-month group randomized trial with specialist-led enriched PE vs. generalist-led conventional PE. Before and after the intervention, Bertsch's Test of Motor Creativity, Movement Assessment Battery for Children, Random Number Generation task and Torrance Test of Creative Thinking were administered. Linear mixed models were run accounting for the random effects of data clusters. Multiple mediation analysis was performed to assess whether motor coordination, executive function and creative thinking mediated any improvement of motor creativity. Results showed that (1) specialist-led enriched PE, compared to generalist-led conventional practice, elicited a more pronounced improvement in all motor creativity dimensions (fluency, flexibility, and originality) independently of baseline levels of motor and cognitive skills and sex; and (2) improved motor creativity was partially mediated by improved motor coordination and, as regards motor flexibility, also by improved inhibitory ability. In conclusion, enriching PE with tailored manipulations of constraints and variability may enhance the ability to create multiple and original task-pertinent movements both directly and through indirect paths. The results are discussed extending to motor creativity a theoretical framework that distinguishes different creativity modes. The intervention may have fostered the generation of creative movements directly through the exposure to variation in constraints, activating the sensorimotor 'flow' mode of creativity that bypasses higher-order cognition, but also indirectly through a systematic and conscious convergence on solutions, activating the 'deliberate' mode of creativity that relies on inhibition to reject common or task-inappropriate movement categories

Enhancing grain size in durum wheat using RNAi to knockdown GW2 genes

Sestili F., Pagliarello R., Zega A., Saletti R., Pucci A., Botticella E., Masci S., Tundo S., Moscetti I., Foti S., Lafiandra D. 2019 Enhancing grain size in durum wheat using RNAi to knock-down GW2 genes. Theoretical and Applied Genetics, 132(2): 419-429 https://doi.org/10.1007/s00122-018-3229-9. Abstract Key message Knocking down GW2 enhances grain size by regulating genes encoding the synthesis of cytokinin, gibberellin, starch and cell wall. Abstract Raising crop yield is a priority task in the light of the continuing growth of the world’s population and the inexorable loss of arable land to urbanization. Here, the RNAi approach was taken to reduce the abundance of Grain Weight 2 (GW2) transcript in the durum wheat cultivar Svevo. The effect of the knockdown was to increase the grains’ starch content by 10–40%, their width by 4–13% and their surface area by 3–5%. Transcriptomic profiling, based on a quantitative real-time PCR platform, revealed that the transcript abundance of genes encoding both cytokinin dehydrogenase 1 and the large subunit of ADP-glucose pyrophosphorylase was markedly increased in the transgenic lines, whereas that of the genes encoding cytokinin dehydrogenase 2 and gibberellin 3-oxidase was reduced. A proteomic analysis of the non-storage fraction extracted from mature grains detected that eleven proteins were differentially represented in the transgenic compared to wild-type grain: some of these were involved, or at least potentially involved, in cell wall development, suggesting a role of GW2 in the regulation of cell division in the wheat grain

Dependability in dynamic, evolving and heterogeneous systems: the CONNECT approach

International audienceThe EU Future and Emerging Technologies (FET) Project Connect aims at dropping the heterogeneity barriers that prevent the eternality of networking systems through a revolutionary approach: to synthesise on-the-y the Connectors via which networked systems communicate. The Connect approach, however, comes at risk from the standpoint of dependability, stressing the need for methods and tools that ensure resilience to faults, errors and malicious attacks of the dynamically Connected system. We are investigating a comprehensive approach, which combines dependability analysis, security enforcement and trust assessment, and is centred around a lightweight adaptive monitoring framework. In this project paper, we overview the research that we are undertaking towards this objective and propose a unifying workflow process that encompasses all the Connect dependability/security/trust concepts and models

SRGeJ045359.9+622444: A 55-min Period Eclipsing AM CVn Discovered from a Joint SRG/eROSITA + ZTF Search

AM CVn systems are ultra-compact binaries where a white dwarf accretes from a helium-rich degenerate or semi-degenerate donor. Some AM CVn systems will be among the loudest sources of gravitational waves for the upcoming Laser Interferometer Space Antenna (LISA), yet the formation channel of AM CVns remains uncertain. We report the study and characterisation of a new eclipsing AM CVn, SRGeJ045359.9+622444 (hereafter SRGeJ0453), discovered from a joint SRG/eROSITA and ZTF program to identify cataclysmic variables (CVs). We obtained optical photometry to confirm the eclipse of SRGeJ0453 and determine the orbital period to be $P_\textrm{orb} = 55.0802 \pm 0.0003$ min. We constrain the binary parameters by modeling the high-speed photometry and radial velocity curves and find $M_\textrm{donor} = 0.044 \pm0.024 M_{\odot}$ and $R_\textrm{donor}=0.078 \pm 0.012 R_{\odot}$. The X-ray spectrum is approximated by a power-law model with an unusually flat photon index of $\Gamma\sim 1$ previously seen in magnetic CVs with SRG/eROSITA, but verifying the magnetic nature of SRGeJ0453 requires further investigation. Optical spectroscopy suggests that the donor star of SRGeJ0453 could have initially been a He star or a He white dwarf. SRGeJ0453 is the ninth eclipsing AM CVn system published to date, and its lack of optical outbursts have made it elusive in previous surveys. The discovery of SRGeJ0453 using joint X-ray and optical surveys highlights the potential for discovering similar systems in the near future.Comment: Submitted to Ap

Multi-wavelength Observations of AT2019wey: a New Candidate Black Hole Low-mass X-Ray Binary

AT2019wey (ATLAS19bcxp, SRGA J043520.9+552226, SRGE J043523.3+552234, ZTF19acwrvzk) is a transient reported by the ATLAS optical survey in December 2019, but shot to fame upon detection, three months later, by the Spektrum-Roentgen-Gamma (SRG) mission in its on-going sky survey. Here we present our ultraviolet, optical, near-infrared and radio observations of this object. Our X-ray observations are reported in a separate paper. We conclude that AT2019wey is a newly discovered Galactic low-mass X-ray binary (LMXB) and a candidate black hole (BH) system. Remarkably, we demonstrate that from ~58950 MJD to ~59100 MJD, despite the significant brightening in radio and X-ray (more than a factor of 10), the optical luminosity of AT2019wey only increased by 1.3--1.4. We interpret the bright UV/optical source in the dim low/hard state (~58950 MJD) as thermal emission from a truncated disk in a hot accretion flow, and the UV/optical emission in the hard-intermediate state (~59100 MJD) as reprocessing of X-ray flux in the outer accretion disk. We discuss the power of combining current wide-field optical surveys and SRG in the discovery of the emerging population of short-period BH LMXB systems with low accretion rates