担子菌の生産するプロテアーゼの検索

Proteolytic activities of culture broth of basidiomycetes were determind. Basidiomycetes used were 12 strains of commercial product and 40 strains of Isolants from nature. Proteolytic activities were found in tamogitake, mushroom and Isolant W510 belonging to Tricholoma sp. In Tricholoma sp.W510, two protease activities having optimal pHs at 7 and 11 were shown. Temperature affected strongly the production of protease during the cultivation. No activity was shown at 30℃. Both protease activities were produced highly by increasing malt extract concentration during cultivation

ソバ種実から得られる低分子性タンパク質のキチン結合活性ならびに抗真菌活性

The low molecular weight proteins which had affinity for chitin were purified in two types of crystalline forms from buckwheat seeds and named as buckwheat chitin-binding proteins I and II, BCP I and II. respectively. To elucidate biological properties of BCP II of them, chitin-binding and antifungal activities of the protein were examined in detail. BCP II possessed the binding specificity for β-1, 4-linked N-acetylglucosamine polysaccharide, chitin, and bound to chitin, reversibly. It was found that the incubation of the protein with chitin at 30℃ and pH 8.0 for 20min was most suitable for the chitin-binding. The equilibrium absorption constant, Ka, and maximum amount of the bound protein, [PC]max, values for binding of BCP II with chitin were estimated to be 0.03 1/μmol and 18.2/μmol/g, respectively. This [PC]max value indicates that approximately 87.3mg of the protein utilizes 1g of chitin for binding. BCP II also showed the growth inhibition against fungi, Saccharomyces, Candida, Aspergillus and Penicillum tested, though the growth of bacteria was not inhibited by the protein, suggesting that the protein may play a important role as defense protein against invasion of plant pathogenic fungi into seeds

担子菌による線溶活性物質生産の培養条件

Cultivation condition of production of fibrinolytic active substance derived from Basidiomycetes was investigated. Basidiomycetes used were 11 strains of commercial product and 30 strains isolated from nature. Fibrinolytic activities were found in Tamogitake of commercial product and 5 strains of Isolants from nature. Isolant W510 strain showed almost the same fibrinolytic activity with Bacillus subtilis, Bacillus natto and Streptomyces griseus. Production of fibrinolytic active substance by W510 strain was high on the glucose media containing potato dextrose, malt extract or cornsteep liqure, but low on the glucose media of yeast extract, soybeen meal digest or polypeptone. Sucrose and corn starch were better carbon sources than glucose. No urea was used for nitrogen source

サイキンニヨルシボウサンタイシャニカンスルケンキュウ

京都大学0048新制・論文博士農学博士乙第2115号論農博第399号新制||農||133(附属図書館)学位論文||S47||N539(農学部図書室)3457UT51-47-K85(主査)教授 緒方 浩一, 教授 秦 忠夫, 教授 栃倉 辰六郎学位規則第5条第2項該当Kyoto UniversityDA

Association between physical activity and change in renal function in patients after acute myocardial infarction.

BackgroundCombined renal dysfunction worsens the subsequent prognosis in patients after acute myocardial infarction (AMI). Therefore, establishing a therapeutic modality to maintain or improve renal function in AMI patients is necessary. This study aimed to elucidate the association between physical activity level and change in renal function in such patients.DesignProspective and observational study.MethodsWe enrolled 41 patients (35 men; average age, 67.5 ± 12.6 years) after AMI onset. Blood biochemistry, urinalysis, and physical function tests were conducted at discharge and 3 months after discharge. Renal function was evaluated based on cystatin C based-estimated glomerular filtration rate (eGFRcys). The number of steps was recorded for 3 months post-discharge. Generalized estimating equations (GEE) was used to test the association between physical activity level and within-patient changes in eGFRcys.ResultsPatients were stratified into low (n = 21; number of steps, 2335 ± 1219 steps/day) and high groups (n = 20; number of steps, 7102 ± 2365 steps/day). eGFRcys significantly increased from baseline to after 3 months in the high group (76.5 ± 13.8 to 83.2 ± 16.0 mL/min/1.73 m2, q = 0.004), whereas no significant change was observed in the low group (65.1 ± 15.9 to 62.2 ± 20.2 mL/min/1.73 m2, q = 0.125). Result of GEE adjusted for potential confounding variables showed a significant positive association between physical activity level and within-patient changes in eGFRcys (p = 0.003). Changes in eGFRcys was -2.9 mL/min/1.73 m2 among low group versus +6.7 mL/min/1.73 m2 among high group.ConclusionsPhysical activity level was positively associated with changes in renal function, demonstrating that high physical activity may suppress renal function decline in patients after AMI

Therapeutic strategy for acute appendicitis based on laparoscopic surgery

Abstract Purpose The treatment strategies for acute appendicitis differ depending on the facility, and various studies have investigated the usefulness of conservative treatment with antibiotics, laparoscopic surgery, and interval appendectomy (IA). However, although laparoscopic surgery is widely used, the clinical strategy for acute appendicitis, especially complicated cases, remains controversial. We assessed a laparoscopic surgery-based treatment strategy for all patients diagnosed with appendicitis, including those with complicated appendicitis (CA). Methods We retrospectively analysed patients with acute appendicitis treated in our institution between January 2013 and December 2021. Patients were classified into uncomplicated appendicitis (UA) and CA groups based on computed tomography (CT) findings on the first visit, and the treatment course was subsequently compared. Results Of 305 participants, 218 were diagnosed with UA and 87 with CA, with surgery performed in 159 cases. Laparoscopic surgery was attempted in 153 cases and had a completion rate of 94.8% (145/153). All open laparotomy transition cases (n = 8) were emergency CA surgery cases. No significant differences were found in the incidence of postoperative complications in successful emergency laparoscopic surgeries. In univariate and multivariate analyses for the conversion to open laparotomy in CA, only the number of days from onset to surgery ≥ 6 days was an independent risk factor (odds ratio: 11.80; P < 0.01). Conclusion Laparoscopic surgery is preferred in all appendicitis cases, including CA. Since laparoscopic surgery is difficult for CA when several days from the onset have passed, it is necessary that surgeons make an early decision on whether to operate